| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,558,537 | C→A | 34.1% | P141P (CCG→CCT) | emrK ← | EmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,558,537 | 0 | C | A | 34.1% | 40.9 / 25.3 | 41 | P141P (CCG→CCT) | emrK | EmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component |
| Reads supporting (aligned to +/- strand): ref base C (17/10); new base A (7/7); total (24/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01 | |||||||||||
ACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATA > minE/1558474‑1558582 | aCTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCa < 1:3420410/68‑1 (MQ=255)aCTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCa < 1:2926422/68‑1 (MQ=255)aCTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCa < 1:2385677/68‑1 (MQ=255) tattaACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTc < 1:137985/68‑1 (MQ=255) tattaACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTc < 1:2603351/68‑1 (MQ=255) tattaACGTATCTTTGGTATGCTCCAGCGCTTCTTTTGAAATAACCCCTTGCTTCGCTAAAGGCAcac < 1:1329654/68‑3 (MQ=255) tattaACGTATCTTTGGTATGCTCCAGCGCTTCTTTTGAAATAACCCCTTGCTTCGCTAAAGGCAcac < 1:593801/68‑3 (MQ=255) tattaACGTATCTTTGGTATGCTCCAGCGCTTCTTTTGAAATAACCCCTTGCTTCGCTAAAGGCAcac < 1:2925479/68‑3 (MQ=255) tattaACGTATCTTTGGTATGCTCCAGCGCTTCTTTTGAAATAACCCCTTGCTTCGCTAAAGGCAcac < 1:2532172/68‑3 (MQ=255) tattaACGTATCTTTGGTATGCTCCAGCGCTTCTTTTGAAATAACCCCTTGCTTCGCTAAAGGCAcac < 1:2226633/68‑3 (MQ=255) tattaACGTATCTTTGGTATGCTCCAGCGCTTCTTTTGAAATAACCCCTTGCTTCGCTAAAGGCAcac < 1:1861090/68‑3 (MQ=255) tattaACGTATCTTTGGTATGCTCCAGCGCTTCTTTTGAAATAACCCCTTGCTTCGCTAAAGGCAcac < 1:1673087/68‑3 (MQ=255) aaCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACgg > 1:979809/1‑59 (MQ=255) aaCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACgg > 1:1530326/1‑59 (MQ=255) aaCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACgg > 1:3153439/1‑59 (MQ=255) aaCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACgg > 1:3087051/1‑59 (MQ=255) aaCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACgg > 1:3084039/1‑59 (MQ=255) aaCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACgg > 1:2688921/1‑59 (MQ=255) aaCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACgg > 1:2411394/1‑59 (MQ=255) aaCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACg > 1:1646379/1‑68 (MQ=255) aaCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACg > 1:1839692/1‑68 (MQ=255) aaCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACg > 1:2304429/1‑68 (MQ=255) aaCGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACg > 1:1843721/1‑68 (MQ=255) aTCTTTGGTATGCTCCAGGGCTTCTTTTGAAATAACCCCTTGCTTCGCTAAAGGCACACGACGGTtata > 1:304207/1‑69 (MQ=255) aTCTTTGGTATGCTCCAGCGCTTCTTTTGAAATAACCCCTTGCTTCGCTAAAGGc > 1:1863320/1‑55 (MQ=255) aTCTTTGGTATGCTCCAGCGCTTCTTTTGAAATAACCCCTTGCTTCGCTAAAGGCACACGATGGTtat > 1:1660258/1‑68 (MQ=255) aTCTTTGGTATGCTCCAGCGCTTCTTTTGAAATAACCCCTTGCTTCGCTAAAGGCACACGACGGTtata > 1:2379192/1‑69 (MQ=255) aTCTTTGGTATGCTCCAGCGCTTCTTTTGAAATAACCCCTTGCTTCGCTAAAGGCACACGACGGTtat > 1:794306/1‑68 (MQ=255) aTCTTTGGTATGCTCCAGCGCTTCTTTTGAAATAACCCCTTGCTTCGCTAAAGGCACACGACGGTtat > 1:641910/1‑68 (MQ=255) aTCTTTCGTATGCTCCAGCGCTTCTTTTGAAATAACCCCTTGCTTCGCTAAAGGCACACGACGGTtat > 1:438027/1‑68 (MQ=255) cTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAg < 1:2246930/68‑1 (MQ=255) cTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAg < 1:884130/68‑1 (MQ=255) cTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGa > 1:428407/1‑69 (MQ=255) cTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGa > 1:2415576/1‑69 (MQ=255) cTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGa > 1:10865/1‑69 (MQ=255) cTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGa > 1:164577/1‑69 (MQ=255) tCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATa > 1:540173/1‑68 (MQ=255) tCTTTTGAAATAACCCCCTGCTTCGCTAACGCCACTCGACGGTTATAATCTTCTAAAGATTGTTGATa > 1:2277848/1‑68 (MQ=255) aaaTAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATa < 1:865928/68‑1 (MQ=255) aaaTAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATa < 1:2566742/68‑1 (MQ=255) tAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATa < 1:2639341/42‑1 (MQ=255) | ACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATA > minE/1558474‑1558582 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |