| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,560,500 | G→A | 39.0% | R169R (CGG→CGA) | evgS → | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,560,500 | 0 | G | A | 39.0% | 42.1 / 52.9 | 82 | R169R (CGG→CGA) | evgS | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Reads supporting (aligned to +/- strand): ref base G (25/25); new base A (11/21); total (36/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.37e-01 | |||||||||||
AACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATC > minE/1560440‑1560560 | aaCCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCaa < 1:982478/68‑1 (MQ=255)aaCCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCaa < 1:1871265/68‑1 (MQ=255)aaCCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCaa < 1:2615372/68‑1 (MQ=255)aaCCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCaa < 1:2868754/68‑1 (MQ=255)aaCCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCaa < 1:1303441/68‑1 (MQ=255)aaCCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCa < 1:2044955/67‑1 (MQ=255)aaCCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCa < 1:2405723/67‑1 (MQ=255)aaCCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCa < 1:1685776/67‑1 (MQ=255)aaCCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCa < 1:1570436/67‑1 (MQ=255)aaCCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCa < 1:3095368/67‑1 (MQ=255)aaCCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCa < 1:1367591/67‑1 (MQ=255)aaCCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCa < 1:1088256/67‑1 (MQ=255)aaCCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCa < 1:96176/67‑1 (MQ=255) accaccCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCa < 1:3108262/66‑1 (MQ=255) accaccCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAAt > 1:2741257/1‑69 (MQ=255) caccCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCa < 1:523041/64‑1 (MQ=255) caccCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCa < 1:2165035/64‑1 (MQ=255) accCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAc > 1:2014905/1‑69 (MQ=255) cTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccc > 1:648517/1‑68 (MQ=255) cTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccc > 1:649373/1‑68 (MQ=255) cTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccc > 1:720578/1‑68 (MQ=255) cTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccc > 1:724204/1‑68 (MQ=255) cTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcc > 1:2740070/1‑67 (MQ=255) cTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGATCGGGTAGCAAATTAccc > 1:358308/1‑68 (MQ=255) cACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCa > 1:3052156/1‑69 (MQ=255) cACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCa > 1:1070967/1‑69 (MQ=255) cACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCa > 1:1270910/1‑69 (MQ=255) tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCatcaatc < 1:1097884/69‑1 (MQ=255) tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCatcaatc < 1:2733984/69‑1 (MQ=255) tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCatcaatc < 1:399491/69‑1 (MQ=255) aCCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCatcaatca > 1:2299155/1‑69 (MQ=255) ccTCATCTAAACCAGTAAATATTGCTCGAGTAGCaaa < 1:459659/37‑1 (MQ=25) ccTCATCAAAACCAGTAAATATTGCTCGAGTAGCaaa < 1:2848389/37‑1 (MQ=38) ccTCATCAAAACCAGTAAATATTGCTCGAGTAGCaaa < 1:2065864/37‑1 (MQ=38) ccTCATCAAAACCAGTAAATATTGCTCGAGTAGCaaa < 1:2977523/37‑1 (MQ=38) ccTCATCAAAACCAGTAAATATTGCTCGAGTAGCaaa < 1:3074132/37‑1 (MQ=38) ccTCATCAAAACCAGTAAATATTGCTCGAGTAGCaaa < 1:3081428/37‑1 (MQ=38) ccTCATCAAAACCAGTAAATATTGCTCGAGTAGCaaa < 1:3183102/37‑1 (MQ=38) ccTCATCAAAACCAGTAAATATTGCTCGAGTAGCaaa < 1:386051/37‑1 (MQ=38) ccTCATCAAAACCAGTAAATATTGCTCGAGTAGCaaa < 1:1245629/37‑1 (MQ=38) ccTCATCAAAACCAGTAAATATTGCTCGAGTAGCaaa < 1:1212875/37‑1 (MQ=38) ccTCATCAAAACCAGTAAATATTGCTCGAGTAGCaaa < 1:666790/37‑1 (MQ=38) ccTCATCAAAACCAGTAAATATTGCTCGAGTAGCaaa < 1:798337/37‑1 (MQ=38) ccTCATCAAAACCAGTAAATATTGCTCGAGTAGCaaa < 1:956730/37‑1 (MQ=38) ccTCATCAAAACCAGTAAATATTGCTCGAGTAGCaaa < 1:1073757/37‑1 (MQ=38) ccTCATCAAAACCAGTAAATATTGCTCGAGTAGCaaa < 1:1039308/37‑1 (MQ=38) ccTCATCAAAACCAGTAAATATTGCTCGAGTAGCaaa < 1:2046834/37‑1 (MQ=38) ccTCATCAAAACCAGTAAATATTGCTCGAGTAGCaaa < 1:2092973/37‑1 (MQ=38) ccTCATCAAAACCAGTAAATATTGCTCGAGTAGCaaa < 1:2145497/37‑1 (MQ=38) ccTCATCAAAACCAGTAAATATTGCTCGAGTAGCaaa < 1:2521488/37‑1 (MQ=38) ccTCATCAAAACCAGTAAATATTGCTCGAGTAGCaaa < 1:261142/37‑1 (MQ=38) ccTCATCAAAACCAGTAAATATTGCTCGAGTAGCaaa < 1:1702333/37‑1 (MQ=38) cTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCAt < 1:3024513/68‑1 (MQ=255) aaaCCAGTAAATATTGCTCGAGTAGCAAATTATCCGCCAGACGAGGTAATTCATCAATCATTTCCaaa > 1:1405377/1‑68 (MQ=255) aaaCCAGTAAATATTGCTCGAGTAGCAAATTATCCCCCAGACGAGGTAATTCATCAATCATTTCCaaa > 1:347378/1‑68 (MQ=255) aaaCCAGTAAATATTGCTCGAGTAGCAAATTATCCCCCAGACGAGGTAATTCATCAATCATTTCCaaa > 1:1122477/1‑68 (MQ=255) aaaCCAGTAAATATTGCTCGAGTAGCAAATTATCCCCCAGACGAGGTAATTCATCAATCATTTCCaaa > 1:682155/1‑68 (MQ=255) aaaCCAGTAAATATTGCTCGAGTAGCAAATTATCCCCCAGACGAGGTAATTCATCAATCATTTCCaaa > 1:390309/1‑68 (MQ=255) aaaCCAGTAAATATTGCTCGAGTAGCAAATTATCCCCCAGACGAGGTAATTCATCAATCATTTCCaaa > 1:351294/1‑68 (MQ=255) aaaCCAGTAAATATTGCTCGAGTAGCAAATTATCCCCCAGACGAGGTAATTCATCAATCATTTCCaaa > 1:2736079/1‑68 (MQ=255) aaaCCAGTAAATATTGCTCGAGTAGCAAATTATCCCCCAGACGAGGTAATTCATCAATCATTTCCaaa > 1:1544434/1‑68 (MQ=255) aaaCCAGTAAATATTGCTCGAGTAGCAAATTATCCCCCAGACGAGGTAATTCATCAATCATTTCCaaa > 1:1525446/1‑68 (MQ=255) aaaCCAGTAAATATTGCTCGAGTAGCAAATTATCCCCCAGACGAGGTAATTCATCAATCATTTCCaaa > 1:299348/1‑68 (MQ=255) aaaCCAGTAAATATTGCTCGAGTAGCAAATTATCCCCCACACGAGGTAATTCATCAATCATTTCCaaa > 1:1769137/1‑68 (MQ=255) aaaTATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAAt < 1:3272372/69‑1 (MQ=255) aaaTATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAAt < 1:3337918/69‑1 (MQ=255) aaaTATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAAt < 1:2844163/69‑1 (MQ=255) aaaTATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAAt < 1:1541983/69‑1 (MQ=255) aaaTATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAAt < 1:2149531/69‑1 (MQ=255) aaTATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTTCAAAAGcaacaa > 1:2190049/1‑67 (MQ=255) aaTATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTTCAAAAGCAACAAt > 1:2926413/1‑68 (MQ=255) aaTATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAAtt > 1:1292572/1‑69 (MQ=255) aaTATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAAtt > 1:2834593/1‑69 (MQ=255) tatTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTa > 1:2032076/1‑68 (MQ=255) tatTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTa > 1:2296698/1‑68 (MQ=255) tatTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTa > 1:1921206/1‑68 (MQ=255) tatTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt > 1:2931176/1‑69 (MQ=255) tatTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt > 1:2440834/1‑69 (MQ=255) tatTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt > 1:301037/1‑69 (MQ=255) tatTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt > 1:305253/1‑69 (MQ=255) tatTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt > 1:1738539/1‑69 (MQ=255) tatTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGC‑ACAATTAtc > 1:3030000/1‑69 (MQ=255) | AACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATC > minE/1560440‑1560560 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |