| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,561,049 | C→T | 36.3% | I352I (ATC→ATT) | evgS → | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,561,049 | 0 | C | T | 36.3% | 35.1 / 29.9 | 55 | I352I (ATC→ATT) | evgS | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Reads supporting (aligned to +/- strand): ref base C (13/22); new base T (6/14); total (19/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGGCGC > minE/1560987‑1561099 | cTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGc > 1:1139391/1‑68 (MQ=255)cTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGc > 1:1550895/1‑68 (MQ=255)cTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGc > 1:2090237/1‑68 (MQ=255)cTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGc > 1:1757019/1‑68 (MQ=255) ttattaCCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCa < 1:1079813/58‑1 (MQ=255) ttattaCCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCa < 1:413381/58‑1 (MQ=255) ttattaCCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCa < 1:850665/58‑1 (MQ=255) ttattaCCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCa < 1:2992425/58‑1 (MQ=255) ttattaCCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCa < 1:2116561/58‑1 (MQ=255) ttattaCCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCa < 1:1376444/58‑1 (MQ=255) ttattaCCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCa < 1:1716603/58‑1 (MQ=255) ttattaCCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCa < 1:1964776/58‑1 (MQ=255) ttattaCCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCa < 1:1890835/58‑1 (MQ=255) ttattaCCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTCCACACAATATCCa < 1:591888/58‑1 (MQ=255) attaCCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATc > 1:1835321/1‑54 (MQ=255) attaCCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATc > 1:717032/1‑54 (MQ=255) attaCCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATc > 1:3335182/1‑54 (MQ=255) attaCCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATc > 1:3101336/1‑54 (MQ=255) attaCCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAg > 1:2826853/1‑69 (MQ=255) attaCCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAg > 1:1975683/1‑69 (MQ=255) attaCCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAg > 1:1707230/1‑69 (MQ=255) attaCCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAg > 1:930588/1‑69 (MQ=255) attaCCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAg > 1:608100/1‑69 (MQ=255) aCAGG‑TTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCggag < 1:2366251/69‑1 (MQ=255) cAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCggag < 1:2702613/69‑1 (MQ=255) cAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCggag < 1:3070791/69‑1 (MQ=255) cAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCggag < 1:2223725/69‑1 (MQ=255) cAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCggag < 1:1883466/69‑1 (MQ=255) cAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCggag < 1:791409/69‑1 (MQ=255) cAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCggag < 1:133349/69‑1 (MQ=255) tttAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCGGAGGAt > 1:3374128/1‑68 (MQ=255) tttAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCGGAGGAt > 1:254356/1‑68 (MQ=255) ttAAATTTTTCTCCGATCACCGTTTCACATAATATTCATGCt < 1:565050/42‑1 (MQ=255) ttAAATTTTTCTCCGATCACCGTTTCACATAATATTCATGCt < 1:681734/42‑1 (MQ=255) ttAAATTTTTCTCCGATCACCGTTTCACATAATATTCATGCt < 1:698674/42‑1 (MQ=255) ttAAATTTTTCTCCGATCACCGTTTCACATAATATTCATGCt < 1:739864/42‑1 (MQ=255) ttAAATTTTTCTCCGATCACCGTTTCACATAATATTCATGCt < 1:32501/42‑1 (MQ=255) ttAAATTTTTCTCCGATCACCGTTTCACATAATATTCATGCt < 1:3107849/42‑1 (MQ=255) ttAAATTTTTCTCCGATCACCGTTTCACATAATATTCATGCt < 1:2393304/42‑1 (MQ=255) ttAAATTTTTCTCCGATCACCGTTTCACATAATATTCATGCt < 1:2940034/42‑1 (MQ=255) ttAAATTTTTCTCCGATCACCGTTTCACATAATATTCATGCt < 1:1825543/42‑1 (MQ=255) ttAAATTTTTCTCCGATCACCGTTTCACATAATATTCATGCt < 1:2914537/42‑1 (MQ=255) ttAAATTTTTCTCCGATCACCGTTTCACATAATATTCATGCt < 1:1915221/42‑1 (MQ=255) ttAAATTTTTCTCCGATCACCGTTTCACATAATATTCATGCt < 1:1229613/42‑1 (MQ=255) ttAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCGGAGGATgg < 1:1334282/69‑1 (MQ=255) ttAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCGGAGGATgg < 1:2225227/69‑1 (MQ=255) ttAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCGGAGGATgg < 1:2908700/69‑1 (MQ=255) tAAATTTTTCTCCGATCACCGTTTCACATAATATTCATGc > 1:200649/1‑40 (MQ=255) tAAATTTTTCTCCGATCACCGTTTCACATAATATTCATGCt < 1:2968925/41‑1 (MQ=255) tAAATTTTTCTCCGATCACCGTTTCACATAATATTCATGCTGGGACACAGCTTAACCCCGGTGGATgg > 1:226175/1‑68 (MQ=255) tAAATTTTTCTCCGATCACCGTTTCACATAATATTCATGCTGGGACACAGCTTAACCCCGGTGGATgg > 1:516805/1‑68 (MQ=255) tAAATTTTTCTCCGATCACCGTTTCACATAATATTCATGCTGGGACACAGCTTAACCCCGGTGGATgg > 1:1002280/1‑68 (MQ=255) tAAATTTTTCTCCGATCACCGTTTCACATAATATTCATGCTGGGACACAGCTTAACCCCGGTGGATgg > 1:2092329/1‑68 (MQ=255) tAAATTTTTCTCCGATCACCGTTTCACATAATATTCATGCTGGGACACAGCTTAACCCCGGTGGATgg > 1:3242945/1‑68 (MQ=255) aTTTTTCTCCGATCACCGTTTCACATAATATTCATGCt < 1:392103/38‑1 (MQ=38) tttttCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCGGAGGATGGGatat < 1:2241453/69‑1 (MQ=255) tttttCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCGGAGGATGGGatat < 1:2245446/69‑1 (MQ=255) cACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGgcgc > 1:971639/1‑69 (MQ=255) cACCGTTTCACACAATATCCAAGCTGGAACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGgcgc > 1:1226410/1‑69 (MQ=255) | CTTAATATTATTACCTTGCAAACAGGTTTAAATTTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGGCGC > minE/1560987‑1561099 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |