Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,562,111 A→C 68.8% I706I (ATA→ATC evgS → hybrid sensory histidine kinase in two‑component regulatory system with EvgA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,562,1110AC68.8% 79.7 / 37.0 64I706I (ATA→ATCevgShybrid sensory histidine kinase in two‑component regulatory system with EvgA
Reads supporting (aligned to +/- strand):  ref base A (15/5);  new base C (21/23);  total (36/28)
Fisher's exact test for biased strand distribution p-value = 5.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

TGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAAC  >  minE/1562046‑1562167
                                                                 |                                                        
tGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAATAAAGCGATAAAg                                                       <  1:1849367/69‑1 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:37567/69‑3 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:1971997/69‑3 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:2084318/69‑3 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:2098117/69‑3 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:2208711/69‑3 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:2756423/69‑3 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:2922008/69‑3 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:3342454/69‑3 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:2494121/69‑3 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:380046/69‑3 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:441445/69‑3 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:45902/69‑3 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:698195/69‑3 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:849487/69‑3 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:945701/69‑3 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:977410/69‑3 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:1819090/69‑3 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:1834511/69‑3 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:114710/69‑3 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:1350440/69‑3 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:1325865/69‑3 (MQ=255)
       atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:1676614/69‑3 (MQ=255)
        tatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg                                                <  1:3155203/68‑3 (MQ=255)
                           cTAATTAATGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCt                             >  1:1192632/1‑68 (MQ=255)
                           cTAATTAATGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCt                             >  1:658483/1‑68 (MQ=255)
                           cTAATTAATGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCt                             >  1:2178050/1‑68 (MQ=255)
                           cTAATTAATGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCt                             >  1:1377843/1‑68 (MQ=255)
                           cTAATTAATGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCt                             >  1:2535064/1‑68 (MQ=255)
                                taatGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAAc                       >  1:3048387/1‑69 (MQ=255)
                                taatGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAAc                       >  1:1665204/1‑69 (MQ=255)
                                taatGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAAc                       >  1:1237235/1‑69 (MQ=255)
                                taatGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAAc                       >  1:459691/1‑69 (MQ=255)
                                taatGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAAc                       >  1:225596/1‑69 (MQ=255)
                                taatGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAAc                       >  1:2201734/1‑69 (MQ=255)
                                taatGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAAc                       >  1:2197818/1‑69 (MQ=255)
                                taatGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAAc                       >  1:74167/1‑69 (MQ=255)
                                 aatGCACTAGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGATTCTGGCAAc                       >  1:461201/1‑68 (MQ=255)
                                   tGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGa                     <  1:1157624/68‑1 (MQ=255)
                                   tGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGa                     <  1:1036409/68‑1 (MQ=255)
                                   tGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGa                     <  1:2584063/68‑1 (MQ=255)
                                          gAGGTAGAAAAATATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCa              >  1:1993970/1‑68 (MQ=255)
                                                  aaaaaaTAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAg     <  1:2712530/69‑1 (MQ=255)
                                                     aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAAt        >  1:2989771/1‑63 (MQ=255)
                                                     aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGaa   >  1:1215069/1‑68 (MQ=255)
                                                     aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGa    >  1:2417020/1‑67 (MQ=255)
                                                     aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac  >  1:1866465/1‑69 (MQ=255)
                                                     aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac  >  1:2957481/1‑69 (MQ=255)
                                                     aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac  >  1:1760338/1‑69 (MQ=255)
                                                     aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac  >  1:804430/1‑69 (MQ=255)
                                                     aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac  >  1:1696350/1‑69 (MQ=255)
                                                     aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac  >  1:1684729/1‑69 (MQ=255)
                                                     aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac  >  1:2099413/1‑69 (MQ=255)
                                                     aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac  >  1:533365/1‑69 (MQ=255)
                                                     aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac  >  1:2193892/1‑69 (MQ=255)
                                                     aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac  >  1:1002794/1‑69 (MQ=255)
                                                     aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac  >  1:410046/1‑69 (MQ=255)
                                                     aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac  >  1:2619377/1‑69 (MQ=255)
                                                     aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac  >  1:356847/1‑69 (MQ=255)
                                                     aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac  >  1:33651/1‑69 (MQ=255)
                                                     aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac  >  1:3205664/1‑69 (MQ=255)
                                                     aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac  >  1:3157993/1‑69 (MQ=255)
                                                     aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac  >  1:2834469/1‑69 (MQ=255)
                                                     aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGACAGTTTCTGGCAACAATGAGTCACGAAATAAGAac  >  1:140822/1‑69 (MQ=255)
                                                                 |                                                        
TGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAAC  >  minE/1562046‑1562167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: