| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,562,114 | G→T | 69.8% | K707N (AAG→AAT) | evgS → | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,562,114 | 0 | G | T | 69.8% | 60.2 / 42.0 | 63 | K707N (AAG→AAT) | evgS | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Reads supporting (aligned to +/- strand): ref base G (15/4); new base T (21/23); total (36/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.77e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01 | |||||||||||
TGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTAT > minE/1562046‑1562182 | tGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAATAAAGCGATAAAg < 1:1849367/69‑1 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:3342454/69‑3 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:1819090/69‑3 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:1834511/69‑3 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:1971997/69‑3 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:2084318/69‑3 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:2098117/69‑3 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:2208711/69‑3 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:2756423/69‑3 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:2922008/69‑3 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:2494121/69‑3 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:37567/69‑3 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:380046/69‑3 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:441445/69‑3 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:45902/69‑3 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:698195/69‑3 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:849487/69‑3 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:945701/69‑3 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:977410/69‑3 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:114710/69‑3 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:1350440/69‑3 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:1676614/69‑3 (MQ=255) atatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:1325865/69‑3 (MQ=255) tatTACCGAGACGCGTGATTTAATTCATGCACTCGAAGTAGAGAGAAATAAAGCGATCAATGCAActg < 1:3155203/68‑3 (MQ=255) cTAATTAATGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCt > 1:1377843/1‑68 (MQ=255) cTAATTAATGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCt > 1:2535064/1‑68 (MQ=255) cTAATTAATGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCt > 1:2178050/1‑68 (MQ=255) cTAATTAATGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCt > 1:1192632/1‑68 (MQ=255) cTAATTAATGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCt > 1:658483/1‑68 (MQ=255) taatGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAAc > 1:459691/1‑69 (MQ=255) taatGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAAc > 1:1665204/1‑69 (MQ=255) taatGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAAc > 1:1237235/1‑69 (MQ=255) taatGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAAc > 1:2197818/1‑69 (MQ=255) taatGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAAc > 1:2201734/1‑69 (MQ=255) taatGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAAc > 1:225596/1‑69 (MQ=255) taatGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAAc > 1:3048387/1‑69 (MQ=255) taatGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAAc > 1:74167/1‑69 (MQ=255) aatGCACTAGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGATTCTGGCAAc > 1:461201/1‑68 (MQ=255) tGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGa < 1:1157624/68‑1 (MQ=255) tGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGa < 1:1036409/68‑1 (MQ=255) tGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGa < 1:2584063/68‑1 (MQ=255) gAGGTAGAAAAATATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCa > 1:1993970/1‑68 (MQ=255) aaaaaaTAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAg < 1:2712530/69‑1 (MQ=255) aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAAt > 1:2989771/1‑63 (MQ=255) aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGaa > 1:1215069/1‑68 (MQ=255) aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGa > 1:2417020/1‑67 (MQ=255) aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac > 1:3205664/1‑69 (MQ=255) aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac > 1:2099413/1‑69 (MQ=255) aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac > 1:410046/1‑69 (MQ=255) aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac > 1:533365/1‑69 (MQ=255) aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac > 1:2193892/1‑69 (MQ=255) aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac > 1:356847/1‑69 (MQ=255) aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac > 1:33651/1‑69 (MQ=255) aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac > 1:1866465/1‑69 (MQ=255) aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac > 1:1684729/1‑69 (MQ=255) aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac > 1:3157993/1‑69 (MQ=255) aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac > 1:1696350/1‑69 (MQ=255) aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac > 1:2957481/1‑69 (MQ=255) aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac > 1:1002794/1‑69 (MQ=255) aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac > 1:804430/1‑69 (MQ=255) aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac > 1:2834469/1‑69 (MQ=255) aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac > 1:1760338/1‑69 (MQ=255) aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGCCAGTTTCTGGCAACAATGAGTCACGAAATAAGAac > 1:2619377/1‑69 (MQ=255) aaaTAAAGCGATCAATGCAACTGTCGCAAAAAGACAGTTTCTGGCAACAATGAGTCACGAAATAAGAac > 1:140822/1‑69 (MQ=255) ggCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCtat < 1:1525825/69‑1 (MQ=255) ggCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCtat < 1:45791/69‑1 (MQ=255) ggCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCtat < 1:389762/69‑1 (MQ=255) ggCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCtat < 1:1343667/69‑1 (MQ=255) ggCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCtat < 1:3339009/69‑1 (MQ=255) ggCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCtat < 1:2479013/69‑1 (MQ=255) ggCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCtat < 1:2868223/69‑1 (MQ=255) ggCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCtat < 1:2565325/69‑1 (MQ=255) ggCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCtat < 1:1063649/69‑1 (MQ=255) ggCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCtat < 1:181499/69‑1 (MQ=255) | TGGCAAGATATTACTGAAACGCGTGATCTAATTAATGCACTCGAGGTAGAAAAAAATAAAGCGATAAAGGCTACCGTAGCAAAAAGTCAGTTTCTGGCAACGATGAGTCACGAAATAAGAACACCAATAAGCTCTAT > minE/1562046‑1562182 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |