| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,565,635 | C→T | 100% | T137T (ACG→ACA) | glk ← | glucokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,565,635 | 0 | C | T | 79.3% | 28.9 / 2.5 | 24 | T137T (ACG→ACA) | glk | glucokinase |
| Reads supporting (aligned to +/- strand): ref base C (5/0); new base T (19/0); total (24/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGGTTCTGCGCCACCAAACTGAATCAGATG > minE/1565577‑1565703 | cGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCAc > 1:2381690/1‑69 (MQ=255) ccAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAgg > 1:3097141/1‑69 (MQ=255) aTCGACATGGACCAGATGCGCAACCCCGAGCCCTGTTCCGGCACCGTAAACCGCAATAGGCtt > 1:687964/1‑63 (MQ=255) aTCGACATGGACCAGATGCGCAACCCCAAGCCCTGTTCCGGCACCGTAAACCGCAATAGGCtt > 1:2125297/1‑63 (MQ=255) aTCGACATGGACCAGATGCGCAACCCCAAGCCCTGTTCCGGCACCGTAAACCGCAATAGGCt > 1:960137/1‑62 (MQ=255) aTCGACATGGACCAGATGCGCAACCCCAAGCCCTGTTCCGGCACCGTAAACCGCAATAGGCt > 1:1846943/1‑62 (MQ=255) gACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCTGTTc > 1:518060/1‑69 (MQ=255) gACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGGtt > 1:2896575/1‑68 (MQ=255) gACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGGTTc > 1:328179/1‑69 (MQ=255) gcgcAACCCCAAGCCCTGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCTAcc > 1:2407332/1‑56 (MQ=255) gcgcAACCCCAAGCCCTGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCTAcc > 1:619540/1‑56 (MQ=255) gcgcAACCCCAAGCCCTGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCTAcc > 1:3271471/1‑56 (MQ=255) gcgcAACCCCAAGCCCTGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCTAcc > 1:3195015/1‑56 (MQ=255) gcgcAACCCCAAGCCCTGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCTAcc > 1:292950/1‑56 (MQ=255) gcgcAACCCCAAGCCCTGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCTAcc > 1:2463883/1‑56 (MQ=255) gcgcAACCCCAAGCCCTGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCTAcc > 1:2352263/1‑56 (MQ=255) gcgcAACCCCAAGCCCTGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCTAcc > 1:21665/1‑56 (MQ=255) gcgcAACCCCAAGCCCTGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCTAcc > 1:196746/1‑56 (MQ=255) gcgcAACCCCAAGCCCTGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCTAcc > 1:1796469/1‑56 (MQ=255) gcgcAACCCCAAGCCCTGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCTAcc > 1:1382287/1‑56 (MQ=255) gcgcAACCCCAAGCCCTGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCTAcc > 1:1271615/1‑56 (MQ=255) gcgcAACCCCAAGCCCTGTTCCGGCACCGTAAACCGCAATAGGCTTAACTTCTAcc > 1:2905941/1‑56 (MQ=255) gcgcAACCCCAAGCCCTGTTCCGGCACCGTAAACAGCAATAGGCTTACCTTcta > 1:769283/1‑52 (MQ=255) gcgcAA‑CCCAAGCCCTGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTc > 1:2706048/1‑51 (MQ=255) cGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGGTTCTGCGCCACCAAACTGAATCATATg < 1:3059752/69‑1 (MQ=255) cGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGGTTCTGCGCCACCAAACTGAATCAGATg < 1:2345259/69‑1 (MQ=255) cGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGGTTCTGCGCCACCAAACTGAATCAGATg < 1:23268/69‑1 (MQ=255) cGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGGTTCTGCGCCACCAAACTGAATCAGATg < 1:1866578/69‑1 (MQ=255) cGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGGTTCTGCGCCACCAAACTGAATCAGATg < 1:1621031/69‑1 (MQ=255) cGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGGTTCTGCGCCACCAAACTGAATCAGATg < 1:1504294/69‑1 (MQ=255) cGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGGTGCTGCGCCACCAAACTGAATCAGATg < 1:1263423/69‑1 (MQ=255) | CGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGGTTCTGCGCCACCAAACTGAATCAGATG > minE/1565577‑1565703 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |