Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,566,500 C→T 65.8% D84D (GAC→GAT yfeO → predicted ion channel protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,566,5000CT65.8% 20.3 / 16.6 35D84D (GAC→GATyfeOpredicted ion channel protein
Reads supporting (aligned to +/- strand):  ref base C (7/5);  new base T (12/11);  total (19/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

TTAACGCTAACGGGTATTGCGGTGGGGTTGGTTATCCGTTTCAGCCAGGGTCATGCCGGACCAGACCCCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTT  >  minE/1566435‑1566557
                                                                 |                                                         
ttAACGCTAACGGGTATTGCGGTGGGGTTGGTTATCCGTTTCAGCCAGGGTCATGCCGGACCAGAcccc                                                        >  1:2360711/1‑69 (MQ=255)
ttAACGCTAACGGGTATTGCGGTGGGGTTGGTTATCCGTTTCAGCCAGGGTCATGCCGGACCAGAcccc                                                        >  1:909137/1‑69 (MQ=255)
ttAACGCTAACGGGTATTGCGGTGGGGTTGGTTATCCGTTTCAGCCAGGGTCATGCCGGACCAGAcccc                                                        >  1:514939/1‑69 (MQ=255)
ttAACGCTAACGGGTATTGCGGTGGGGTTGGTTATCCGTTTCAGCCAGGGTCATGCCGGACCAGAcccc                                                        >  1:2026808/1‑69 (MQ=255)
ttAACGCTAACGGGTATTGCGGTGGGGTTGGTTATCCGTTTCAGCCAGGGTCATGCCGGACCAGAcccc                                                        >  1:2802496/1‑69 (MQ=255)
     gCTAACGGGTATTGCGGTGGGGTTGGTTATCCGTTTCAGCCAGGGTCATGCCGGACCAGACCCCGCCtg                                                   <  1:1705780/69‑1 (MQ=255)
                       ggggTTGGTTATCCGTTTCAGCCAGGGTCATGCCGGACCAGACCCCGCCTGTGAACCGCTGATCGgcgc                                 >  1:187484/1‑69 (MQ=255)
                          gttggttATCCGTTTCAGCCAGGGTCATGCCGGACCAGAcccc                                                        >  1:3417662/1‑43 (MQ=255)
                          gttggttATCCGTTTCAGCCAGGGTCATGCCGGACCAGACCCCGCCTGTGAACCGCTGATCGGCGCAcc                              >  1:1859532/1‑69 (MQ=255)
                          gttggttATCCGTTTCAGCCAGGGTCATGCCGGACCAGACCCCGCCTGTGAACCGCTGATCGGCGCAcc                              >  1:246528/1‑69 (MQ=255)
                          gttggttATCCGTTTCAGCCAGGGTCATGCCGGACCAGACCCCGCCTGTGAACCGCTGATCGGCGCAcc                              >  1:1470530/1‑69 (MQ=255)
                          gttggttATCCGTTTCAGCCAGGGTCATGCCGGACCAGACCCCGCCTGTGAACCGCTGATCGGCGCAcc                              >  1:1217746/1‑69 (MQ=255)
                             ggttATCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCgat                           <  1:1231517/69‑3 (MQ=255)
                             ggttATCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCgat                           <  1:320511/69‑3 (MQ=255)
                             ggttATCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCgat                           <  1:3308381/69‑3 (MQ=255)
                             ggttATCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCgat                           <  1:2757073/69‑3 (MQ=255)
                             ggttATCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCgat                           <  1:2705389/69‑3 (MQ=255)
                             ggttATCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCgat                           <  1:404531/69‑3 (MQ=255)
                             ggttATCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCgat                           <  1:2328132/69‑3 (MQ=255)
                             ggttATCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCgat                           <  1:1407204/69‑3 (MQ=255)
                             ggttATCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCgat                           <  1:1765757/69‑3 (MQ=255)
                             ggttATCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCgat                           <  1:1991202/69‑3 (MQ=255)
                             ggttATCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCgat                           <  1:1771499/69‑3 (MQ=255)
                                 aTCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCtgtg                                                 >  1:2620221/1‑43 (MQ=255)
                                 aTCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCGATTCCg                       >  1:2299191/1‑69 (MQ=255)
                                 aTCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCGATTCCg                       >  1:1324305/1‑69 (MQ=255)
                                 aTCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCGATTCCg                       >  1:504947/1‑69 (MQ=255)
                                 aTCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCGATTCCg                       >  1:477499/1‑69 (MQ=255)
                                 aTCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCGATTCCg                       >  1:3391899/1‑69 (MQ=255)
                                 aTCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCGATTCCg                       >  1:1918265/1‑69 (MQ=255)
                                 aTCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCGATTCCg                       >  1:308161/1‑69 (MQ=255)
                                 aTCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCGATTCCg                       >  1:2028885/1‑69 (MQ=255)
                                 aTCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCGATTCCg                       >  1:2279659/1‑69 (MQ=255)
                                 aTCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCGATTCCg                       >  1:2698243/1‑69 (MQ=255)
                                 aTCCGTTTCAGCCAGGGTCATGCCGGACCAGATCCCGCCTGTGAACCGCTTATCGGCGCACCGATTCCg                       >  1:2286124/1‑69 (MQ=255)
                                            ccAGGGTCATGCCGGACCAGACCCCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCt            <  1:247947/69‑1 (MQ=255)
                                            ccAGGGTCATGCCGGACCAGACCCCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCt            <  1:801913/69‑1 (MQ=255)
                                            ccAGGGGCATGCCGGACCAGACCCCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCt            <  1:928169/69‑1 (MQ=255)
                                                  tCATGCCGGACCAGACCCCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTgg      >  1:138858/1‑69 (MQ=255)
                                                  tCATGCCGGACCAGACCCCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTgg      >  1:587722/1‑69 (MQ=255)
                                                      gCCGGACCAGACCCCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACtt  <  1:3415428/69‑1 (MQ=255)
                                                                 |                                                         
TTAACGCTAACGGGTATTGCGGTGGGGTTGGTTATCCGTTTCAGCCAGGGTCATGCCGGACCAGACCCCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTT  >  minE/1566435‑1566557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: