| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,566,887 | T→G | 40.2% | A213A (GCT→GCG) | yfeO → | predicted ion channel protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,566,887 | 0 | T | G | 40.2% | 40.4 / 93.7 | 97 | A213A (GCT→GCG) | yfeO | predicted ion channel protein |
| Reads supporting (aligned to +/- strand): ref base T (41/17); new base G (15/24); total (56/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.08e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGC > minE/1566822‑1566952 | gcggcAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCAt > 1:100489/1‑69 (MQ=255)gcggcAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCAt > 1:1583027/1‑69 (MQ=255)gcggcAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCAt < 1:2485429/69‑1 (MQ=255)gcggcAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCAt < 1:2361853/69‑1 (MQ=255)gcggcAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCAt > 1:319757/1‑69 (MQ=255)gcggcAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCAt < 1:3119039/69‑1 (MQ=255)gcggcAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCAt < 1:742517/69‑1 (MQ=255)gcggcAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCAt < 1:670758/69‑1 (MQ=255)gcggcAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCAt < 1:3151994/69‑1 (MQ=255)gcggcAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATACTCATTTTTCACTGCCCATTGCTCAt > 1:3401155/1‑69 (MQ=255) agcagcTGGTGCACTTACTACCGGATTATTTTTCCATTCTCATTTTTCACTGCCCATTGCGCATTACg > 1:2663870/1‑68 (MQ=255) agcagcTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCAt > 1:412761/1‑64 (MQ=255) agcagcTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACg > 1:3308556/1‑68 (MQ=255) agcagcTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACg > 1:3333841/1‑68 (MQ=255) agcagcTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACg > 1:391703/1‑68 (MQ=255) agcagcTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACg > 1:506284/1‑68 (MQ=255) agcagcTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACg > 1:573592/1‑68 (MQ=255) agcagcTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACg > 1:1965741/1‑68 (MQ=255) agcagcTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACg > 1:2792678/1‑68 (MQ=255) agcagcTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACg > 1:2758269/1‑68 (MQ=255) agcagcTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACg > 1:1183032/1‑68 (MQ=255) agcagcTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACg > 1:810128/1‑68 (MQ=255) agcagcTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACg > 1:91674/1‑68 (MQ=255) agcagcTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACg > 1:253760/1‑68 (MQ=255) agcagcTGGTGCACTTACTACCGGATTATTTTTCCATACTCATTTTTCACTGCCCATTGCGCATTACg > 1:2569039/1‑68 (MQ=255) tactacCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGa > 1:118915/1‑68 (MQ=255) actacCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGAcc > 1:1539849/1‑69 (MQ=255) actacCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGAcc > 1:996496/1‑69 (MQ=255) actacCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGAcc > 1:2041552/1‑69 (MQ=255) actacCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGAcc > 1:759229/1‑69 (MQ=255) actacCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGAcc > 1:2219480/1‑69 (MQ=255) actacCGGATTATTTTTACATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGAcc > 1:1457524/1‑69 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:3110840/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:2943437/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:746025/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:761513/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:79292/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:2739637/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:313532/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:862324/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:909178/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:662536/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:2590235/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:2466680/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:1942771/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:2112215/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:1777412/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:1616253/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:2262058/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:1460185/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:2386191/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:1324713/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:1127290/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:2546862/67‑1 (MQ=255) ggATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:1024499/67‑1 (MQ=255) gATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCGCATTACGGACAGATGGAAATGACCGata < 1:1976720/66‑1 (MQ=255) cTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTg > 1:718424/1‑69 (MQ=255) cTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTg > 1:1435025/1‑69 (MQ=255) cTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTg > 1:2564390/1‑69 (MQ=255) cTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTg > 1:1200087/1‑69 (MQ=255) cTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTg > 1:113564/1‑69 (MQ=255) cTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTg > 1:1605342/1‑69 (MQ=255) cTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTg > 1:875911/1‑69 (MQ=255) cTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTg > 1:968730/1‑69 (MQ=255) cTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTg > 1:331439/1‑69 (MQ=255) cTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTg > 1:182747/1‑69 (MQ=255) cTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTg > 1:2662486/1‑69 (MQ=255) cTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTg > 1:268052/1‑69 (MQ=255) cTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTg > 1:2775394/1‑69 (MQ=255) cTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTg > 1:3037430/1‑69 (MQ=255) tttttCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTcgc > 1:1580296/1‑69 (MQ=255) tttttCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTcgc > 1:1318317/1‑69 (MQ=255) tttttCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTcgc > 1:1494812/1‑69 (MQ=255) tttttCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTcgc > 1:867407/1‑69 (MQ=255) tttttCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTcgc > 1:3169503/1‑69 (MQ=255) tttttCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTcg > 1:2569895/1‑68 (MQ=255) tttttCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTcg > 1:965307/1‑68 (MQ=255) tCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCgcggc < 1:3426854/68‑1 (MQ=255) tCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCgcggc < 1:3369616/68‑1 (MQ=255) tCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCgcggc < 1:1062360/68‑1 (MQ=255) tCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCgcggc < 1:868167/68‑1 (MQ=255) cTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGa < 1:2179370/67‑1 (MQ=255) gCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGc < 1:2702961/69‑1 (MQ=255) gCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGc < 1:2527070/69‑1 (MQ=255) gCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGc < 1:2570227/69‑1 (MQ=255) gCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGc < 1:402240/69‑1 (MQ=255) gCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGc < 1:3326400/69‑1 (MQ=255) gCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGc < 1:203783/69‑1 (MQ=255) gCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAAt > 1:1310218/1‑47 (MQ=255) gCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATc > 1:427000/1‑66 (MQ=255) gCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCgc > 1:1060044/1‑68 (MQ=255) gCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCgc > 1:2562767/1‑68 (MQ=255) gCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCgc > 1:2654187/1‑68 (MQ=255) gCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCgc > 1:1196851/1‑68 (MQ=255) gCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCgc > 1:1314038/1‑68 (MQ=255) gCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCgc > 1:2487059/1‑68 (MQ=255) gCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCgc > 1:2345319/1‑68 (MQ=255) | GCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGC > minE/1566822‑1566952 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |