| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,571,749 | A→G | 31.3% | V75V (GTA→GTG) | yfeC → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,571,749 | 0 | A | G | 31.3% | 90.9 / 53.0 | 105 | V75V (GTA→GTG) | yfeC | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (27/45); new base G (2/31); total (29/76) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.55e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01 | |||||||||||
Only 100 of 109 total aligned reads displayed.
GCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTT > minE/1571687‑1571812 | gCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTg > 1:692673/1‑69 (MQ=255)gCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTg > 1:2697817/1‑69 (MQ=255) cTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGTGCCGCTTGAAGTATTGCTg > 1:907239/1‑68 (MQ=255) cTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTg > 1:2525965/1‑68 (MQ=255) cTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTg > 1:811394/1‑68 (MQ=255) aCGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGac > 1:2590210/1‑69 (MQ=255) aCGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa > 1:1606966/1‑68 (MQ=255) aCGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa > 1:3348284/1‑68 (MQ=255) aCGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTCCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGac > 1:2065091/1‑69 (MQ=255) cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTTATGCGCCGCTTGAAGTATTGCTGGTGa < 1:796450/67‑1 (MQ=255) cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa < 1:2110403/67‑1 (MQ=255) cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa < 1:2468236/67‑1 (MQ=255) cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa < 1:2390352/67‑1 (MQ=255) cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa < 1:2046207/67‑1 (MQ=255) cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa < 1:529437/67‑1 (MQ=255) cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa < 1:2469950/67‑1 (MQ=255) cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa < 1:237948/67‑1 (MQ=255) cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa < 1:454915/67‑1 (MQ=255) cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa < 1:383985/67‑1 (MQ=255) cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa < 1:1558251/67‑1 (MQ=255) cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa < 1:3033358/67‑1 (MQ=255) cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa < 1:2602498/67‑1 (MQ=255) cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa < 1:3287174/67‑1 (MQ=255) cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa < 1:1018115/67‑1 (MQ=255) cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa < 1:1569058/67‑1 (MQ=255) cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa < 1:772311/67‑1 (MQ=255) gTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa < 1:1067759/66‑1 (MQ=255) tCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACt < 1:1059014/69‑1 (MQ=255) cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAGGTATTGCTGGTGACACTg > 1:1766079/1‑68 (MQ=255) cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTg > 1:1814462/1‑68 (MQ=255) cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTg > 1:1463611/1‑68 (MQ=255) cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTg > 1:1780275/1‑68 (MQ=255) cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTg > 1:2519888/1‑68 (MQ=255) cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTg > 1:2074288/1‑68 (MQ=255) cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTg > 1:3384073/1‑68 (MQ=255) cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTg > 1:3395482/1‑68 (MQ=255) cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTg > 1:1729483/1‑68 (MQ=255) cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTg > 1:748755/1‑68 (MQ=255) cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGGGATGCGCCGCTTGAAGTATTGCTGGTGACACTg > 1:2658302/1‑68 (MQ=255) aaGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGa < 1:268276/69‑1 (MQ=255) aaGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGa < 1:2134983/69‑1 (MQ=255) aaGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGa < 1:2316579/69‑1 (MQ=255) aaGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGa < 1:1066165/69‑1 (MQ=255) aaGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGa < 1:1974525/69‑1 (MQ=255) aaGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGa < 1:1356098/69‑1 (MQ=255) aGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATT‑CTGGTGACACTGGCGAAAGAGAt < 1:2614714/69‑1 (MQ=255) ggAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGAt < 1:316423/68‑1 (MQ=255) ggAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGAt < 1:298991/68‑1 (MQ=255) ggAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGAt < 1:2449516/68‑1 (MQ=255) ggAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGAt < 1:933882/68‑1 (MQ=255) ggAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGAt < 1:1511674/68‑1 (MQ=255) aGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCgt < 1:1270568/56‑2 (MQ=255) ttCTGGTGATGCGCCGCTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACaaa > 1:435523/1‑68 (MQ=255) ttCTGGTGATGCGCCGCTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACaaa > 1:722693/1‑68 (MQ=255) ttCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACaaaa < 1:2223567/69‑1 (MQ=255) ggTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACa < 1:2910535/68‑1 (MQ=255) aTGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa > 1:1517336/1‑69 (MQ=255) aTGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa > 1:1775724/1‑69 (MQ=255) aTGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa < 1:1218522/69‑1 (MQ=255) aTGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa > 1:2458571/1‑69 (MQ=255) aTGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa > 1:3311094/1‑69 (MQ=255) aTGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa < 1:1072916/69‑1 (MQ=255) aTGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa < 1:2205278/69‑1 (MQ=255) aTGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa < 1:3141731/69‑1 (MQ=255) aTGCGCCGCTTGAAGTATTGCTGGGGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa < 1:254806/69‑1 (MQ=255) tGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa < 1:3145465/68‑1 (MQ=255) cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt < 1:976351/69‑1 (MQ=255) cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt < 1:962780/69‑1 (MQ=255) cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt < 1:554928/69‑1 (MQ=255) cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt < 1:2218514/69‑1 (MQ=255) cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt < 1:1027111/69‑1 (MQ=255) cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt < 1:1161262/69‑1 (MQ=255) cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt < 1:1504543/69‑1 (MQ=255) cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt < 1:1976112/69‑1 (MQ=255) cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt < 1:2303244/69‑1 (MQ=255) cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt < 1:2342770/69‑1 (MQ=255) cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt < 1:2959134/69‑1 (MQ=255) cgcTTGAAGTATTGCTGGTGACACTGGCGAAATAGAt < 1:3421112/37‑1 (MQ=255) gcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt < 1:3144825/68‑1 (MQ=255) aGTGTTGCTGGTGACACTGGCGAAAGATATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt < 1:2259651/67‑1 (MQ=255) aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt < 1:560402/67‑1 (MQ=255) aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt < 1:3116194/67‑1 (MQ=255) aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt < 1:1096764/67‑1 (MQ=255) aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt < 1:1107547/67‑1 (MQ=255) aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt < 1:1122568/67‑1 (MQ=255) aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt < 1:3083636/67‑1 (MQ=255) aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt < 1:1184648/67‑1 (MQ=255) aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt < 1:3258169/67‑1 (MQ=255) aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt < 1:765076/67‑1 (MQ=255) aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt < 1:1622841/67‑1 (MQ=255) aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt < 1:332428/67‑1 (MQ=255) aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt < 1:556323/67‑1 (MQ=255) aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt < 1:532489/67‑1 (MQ=255) aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt < 1:2994725/67‑1 (MQ=255) aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt < 1:2447181/67‑1 (MQ=255) aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt < 1:2888264/67‑1 (MQ=255) aTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa > 1:1623199/1‑53 (MQ=255) aTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa > 1:3033073/1‑53 (MQ=255) aTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa > 1:2137276/1‑53 (MQ=255) aTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa > 1:3319931/1‑53 (MQ=255) | GCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTT > minE/1571687‑1571812 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |