| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,577,902 | A→T | 39.1% | F12Y (TTT→TAT) | xapA ← | purine nucleoside phosphorylase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,577,902 | 0 | A | T | 39.1% | 41.3 / 62.8 | 69 | F12Y (TTT→TAT) | xapA | purine nucleoside phosphorylase II |
| Reads supporting (aligned to +/- strand): ref base A (25/17); new base T (9/18); total (34/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
ATAAAGGCCACTCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCTGAGACATACATATCCTTTTTCTGTAGGGTGGAATCTAA > minE/1577845‑1577967 | aTAAAGGCCACTCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGt < 1:2468356/67‑1 (MQ=255) gCCACTCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGt < 1:675147/61‑1 (MQ=255) gCCACTCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTACGCAATACAGTGGGTTATGAGaa < 1:352833/69‑1 (MQ=255) gCCACTCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTACGCAATACAGTGGGTTATGAGaa < 1:2594049/69‑1 (MQ=255) gCCACTCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTACGCAATACAGTGGGTTATGAGaa < 1:1830426/69‑1 (MQ=255) gCCACTCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTACGCAATACAGTGGGTTATGAGaa < 1:259967/69‑1 (MQ=255) gCCACTCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTACGCAATACAGTGGGTTATGAGaa < 1:2865268/69‑1 (MQ=255) gCCACTCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTACGCAATACAGTGGGTTATGAGaa < 1:3044591/69‑1 (MQ=255) gCCACTCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTACGCAATACAGTGGGTTATGAGaa < 1:172741/69‑1 (MQ=255) gCCACTCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTACGCAATACAGTGGGTTATGAGaa < 1:248808/69‑1 (MQ=255) gCCACTCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTACGCAATACAGTGGGTTATGAGaa < 1:1702406/69‑1 (MQ=255) gCCACTCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTACGCAATACAGTGGGTTATGAGaa < 1:2433898/69‑1 (MQ=255) gCCACTCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTACGCAATACAGTGGGTTATGAGaa < 1:2056593/69‑1 (MQ=255) gCCACTCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTACGCAATACAGTGGGTTATGAGaa < 1:449275/69‑1 (MQ=255) gCCACTCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTACGCAATACAGTGGGTTATGAGaa < 1:2096085/69‑1 (MQ=255) gCCACTCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTACGCAATACAGTGGGTTATGAGaa < 1:54273/69‑1 (MQ=255) gCCACTCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTACGCAATACAGTGGGTTATGAGaa < 1:2216389/69‑1 (MQ=255) gCCACTCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTACGCAATACAGTGGGTTATGAGaa < 1:2221754/69‑1 (MQ=255) gCCACTCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTACGCAATACAGTGGGTTATGAGaa < 1:962850/69‑1 (MQ=255) tCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTATGCAAAAGAGCGGGTTATGAGAAAATTg > 1:1276208/1‑69 (MQ=255) tCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAAtt > 1:3107760/1‑68 (MQ=255) tCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAAtt > 1:106172/1‑68 (MQ=255) tCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTg > 1:1715939/1‑69 (MQ=255) tCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTg > 1:3190125/1‑69 (MQ=255) tCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTg > 1:3226250/1‑69 (MQ=255) tCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTg > 1:442403/1‑69 (MQ=255) tCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTg > 1:462521/1‑69 (MQ=255) tCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTg > 1:799191/1‑69 (MQ=255) cGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTATTCAAAACAGCGGGTTATGAGAAAATTGa < 1:736637/69‑1 (MQ=255) ggCGTGAAAATCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTGaa < 1:2316559/68‑1 (MQ=255) ggCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTATGCaaa > 1:966154/1‑43 (MQ=255) ggCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTGAAc > 1:1626320/1‑68 (MQ=255) ggCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTGAAc > 1:1802059/1‑68 (MQ=255) gCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCt < 1:1764521/69‑1 (MQ=255) gCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCt < 1:1768510/69‑1 (MQ=255) gTG‑AAATCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCTga < 1:861769/69‑1 (MQ=255) g‑AAATCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCTgaga < 1:3064244/69‑1 (MQ=255) aaaTCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCTgaga < 1:559471/68‑1 (MQ=255) aTCAGGTTTATAAGTCTTGATAATATATATGCAAAACAGCGGGTTATGAGAAAATTGAACCTGAGaca < 1:2412777/68‑1 (MQ=255) tCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCTGAGacata < 1:1878392/69‑1 (MQ=255) tCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCTGAGacata < 1:3297497/69‑1 (MQ=255) tCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCTGAGacata < 1:2718016/69‑1 (MQ=255) tCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCTGAGacata < 1:1116810/69‑1 (MQ=255) tCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCTGAGacata < 1:849915/69‑1 (MQ=255) tCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCTGAGacata < 1:1520568/69‑1 (MQ=255) tCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCTGAGacata < 1:1717398/69‑1 (MQ=255) cAGGTTTATAAGTCTTGATAATATCTATGCAAAAc > 1:1878307/1‑35 (MQ=255) cAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCTGAGacata > 1:2527048/1‑68 (MQ=255) cAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCTGAGacata > 1:2614035/1‑68 (MQ=255) cAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCTGAGacata > 1:3057044/1‑68 (MQ=255) aGGTTTATAAGTCTTGATAATATCTACGCAATACAGTGGGTTATGAGaa < 1:1248341/49‑1 (MQ=255) atatCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCTGAGACATACATATCCTTTTTCTGTAggg > 1:1074943/1‑68 (MQ=255) tatCTACGCAATACAGTGGGTTATGAGAAAACTGAGCCTGATacatatat > 1:2263895/1‑50 (MQ=255) tatCTACGCAATACAGTGGGTTATGAGAAAACTGAGCCTGATacatatat > 1:1904272/1‑50 (MQ=255) tatCTACGCAATACAGTGGGTTATGAGAAAACTGAGCCTGATAcatatata > 1:1712399/1‑51 (MQ=255) tatCTACGCAATACAGTGGGTTATGAGAAAACTGAGCCTGATAcatatata > 1:3011842/1‑51 (MQ=255) tatCTACGCAATACAGTGGGTTATGAGAAAACTGAGCCTGATAcatatata > 1:1587245/1‑51 (MQ=255) tatCTACGCAATACAGTGGGTTATGAGAAAACTGAGCCTGATAcatatata > 1:615637/1‑51 (MQ=255) tatCTACGCAATACAGTGGGTTATGAGAAAACTGAGCCTGATAcatatata > 1:1535885/1‑51 (MQ=255) tatCTACGCAATACAGTGGGTTATGAGAAAACTGAGCCTGATAcatatata > 1:2410769/1‑51 (MQ=255) tatCTACGCAATACAGTGGGTTATGAGAAAACTGAGCCTGATAcatatata > 1:1448237/1‑51 (MQ=255) tCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCTGAGacata > 1:3097171/1‑45 (MQ=255) tCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCTGAGacata > 1:2942010/1‑45 (MQ=255) tCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCTGAGacata > 1:2560810/1‑45 (MQ=255) tCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCTGAGACATACATATCCTTTTTCTGTAGGGTGGa > 1:1333117/1‑69 (MQ=255) tGCAAAACAGCGGGTTATGAGAAAATTGAACCTGAGACATACATATCCTTTTTCTGTAGGGTGGAATCt > 1:2408695/1‑69 (MQ=255) cAAAACAGCGGGTTATGAGAAAATTGAACCTGAGACATACATATCCTTTTTCTGTAGGGTGGAATCTaa > 1:1575435/1‑69 (MQ=255) cAAAACAGCGGGTTATGAGAAAATTGAACCTGAGACATACATATCCTTTTTCTGTAGGGTGGAATCTaa > 1:3105313/1‑69 (MQ=255) cAAAACAGCGGGTTATGAGAAAATTGAACCTGAGACATACATATCCTTTTTCTGTAGGGTGGAATCTaa > 1:1135216/1‑69 (MQ=255) cAAAACAGCGGGTTATGAGAAAATTGAACCTGAGACATACATATCCTTTTTCTGTAGGGTGGAATCTaa > 1:1935356/1‑69 (MQ=255) | ATAAAGGCCACTCGTGGCGTG‑AAATCAGGTTTATAAGTCTTGATAATATCTATGCAAAACAGCGGGTTATGAGAAAATTGAACCTGAGACATACATATCCTTTTTCTGTAGGGTGGAATCTAA > minE/1577845‑1577967 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |