| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,581,564 | T→G | 55.4% | A557A (GCA→GCC) | ligA ← | DNA ligase, NAD(+)‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,581,564 | 0 | T | G | 55.4% | 37.6 / 68.4 | 76 | A557A (GCA→GCC) | ligA | DNA ligase, NAD(+)‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (5/29); new base G (13/29); total (18/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGGCTTCCAGCGCTTCCAG > minE/1581503‑1581632 | ccAACAGCTCGCTGATGACATTGCGGTT‑CTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:2936727/69‑1 (MQ=255) cAACAGCTCGCTGATGACTTTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:3264611/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGAc < 1:3071413/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGAc < 1:1869190/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGAc < 1:1782036/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGAc < 1:1644199/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGAc < 1:3282573/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGAc < 1:1570981/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGAc < 1:3247938/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGAc < 1:2796848/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGAc < 1:1187009/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGAc < 1:3013635/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:2790233/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:2821342/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:2871174/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:2872228/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:2595254/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:3350091/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:2554458/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:345490/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:449040/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:102136/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:481145/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:588688/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:1099652/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:1915017/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:700757/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:1814563/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:1809998/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:1376153/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:704670/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:1478103/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:1562674/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:888900/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTCGGAGGCAACGAc < 1:651279/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTCGGAGGCAACGAc < 1:3142530/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTCGTGAACGTGGGAGGCAACGAc < 1:2839168/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGGGTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGAc < 1:390599/69‑1 (MQ=255) cAACAGCTCGCTGATGACATTGCGATTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:952501/69‑1 (MQ=255) aaCAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGAGGCAACGAc < 1:955159/68‑1 (MQ=255) tCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGAc < 1:1223001/62‑1 (MQ=255) aTTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCa < 1:730934/68‑1 (MQ=255) aTTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCa < 1:1289232/68‑1 (MQ=255) aTTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCa < 1:1980493/68‑1 (MQ=255) gCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGc > 1:1113105/1‑42 (MQ=255) ttGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGc < 1:837041/69‑1 (MQ=255) ttGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGc < 1:3319419/69‑1 (MQ=255) ttGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGc < 1:342166/69‑1 (MQ=255) ttGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGc < 1:3080980/69‑1 (MQ=255) ttGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGc < 1:1075091/69‑1 (MQ=255) ttGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGc < 1:2017440/69‑1 (MQ=255) ttGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGc < 1:2167746/69‑1 (MQ=255) ttGCTTTCTTCGGCAAAGAAGTTATGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGc < 1:934394/69‑1 (MQ=255) tagCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGc < 1:1899740/67‑1 (MQ=255) tGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGc < 1:599103/68‑1 (MQ=255) tGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGc < 1:2587250/68‑1 (MQ=255) tGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGc < 1:1606303/68‑1 (MQ=255) tGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCa > 1:872338/1‑69 (MQ=255) cGGCAAAGAAGTTGTGAACGTGGGAGGCAACGACAATTCCAACATCAGGCACCTTTTGCAGCTCTTCaa > 1:831588/1‑69 (MQ=255) cGGCAAAGAAGTTGTGAACGTGGGAGGCAACGACAATTCCAACATCAGGCACCTTTTGCAGCTCTTCaa > 1:685809/1‑69 (MQ=255) cGGCAAAGAAGTTGTGAACGTGGGAGGCAACGACAATTCCAACATCAGGCACCTTTTGCAGCTCTTCaa > 1:1724011/1‑69 (MQ=255) cGGCAAAGAAGTTGTGAACGTGGGAGGCAACGACAATTCCAACATCAGGCACCTTTTGCAGCTCTTCaa > 1:738491/1‑69 (MQ=255) cGGCAAAGAAGTTGTGAACGTGGGAGGCAACGACAATTCCAACATCAGGCACCTTTTGCAGCTCTTCaa > 1:489524/1‑69 (MQ=255) cGGCAAAGAAGTTGTGAACGTGGGAGGCAACGACAATTCCAACATCAGGCACCTTTTGCAGCTCTTCaa > 1:2416576/1‑69 (MQ=255) cGGCAAAGAAGTTGTGAACGTGGGAGGCAACGACAATTCCAACATCAGGCACCTTTTGCAGCTCTTCaa > 1:2512163/1‑69 (MQ=255) cGGCAAAGAAGTTGTGAACGTGGGAGGCAACGACAATTCCAACATCAGGCACCTTTTGCAGCTCTTCaa > 1:2616740/1‑69 (MQ=255) cGGCAAAGAAGTTGTGAACGTGGGAGGCAACGACAATTCCAACATCAGGCACCTTTTGCAGCTCTTCaa > 1:3274796/1‑69 (MQ=255) cGGCAAAGAAGTTGTGAACGTGGGAGGCAACGACAATTCCAACATCAGGCACCTTTTGCAGCTCTTCaa > 1:2697358/1‑69 (MQ=255) cGGCAAAGAAGTTGTGAACGTGGGAGGCAACGACAATTCCAACATCAGGCACCTTTTGCAGCTCTTCaa > 1:3033529/1‑69 (MQ=255) cGGCAAAGAAGTTGTGAACGTGGGAGGCAACGACAATTCCAACATCAGGCACCTTTTGCAGCTCTTCaa > 1:2882275/1‑69 (MQ=255) cGGCAAAGAAGTTGTGAACGTGGGAGGCAACGACAATTCCAACATCAGGCACCTTTTGCAGCTCTTCa > 1:1431911/1‑68 (MQ=255) aaaGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGa > 1:988134/1‑69 (MQ=255) aagTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCg < 1:901578/69‑1 (MQ=255) aagTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCg < 1:3172324/69‑1 (MQ=255) gtgAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGgct > 1:21342/1‑67 (MQ=255) gtgAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGgct > 1:3240074/1‑67 (MQ=255) tGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGgcttccagcgcttccag > 1:655156/1‑69 (MQ=255) tGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGgcttccagcgcttccag > 1:1713984/1‑69 (MQ=255) tGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGgcttccagcgcttccag > 1:573750/1‑69 (MQ=255) tGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGgcttccagcgcttccag > 1:2538499/1‑69 (MQ=255) tGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGgcttccagcgcttccag > 1:1671556/1‑69 (MQ=255) tGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGgcttccagcgcttccag > 1:2545262/1‑69 (MQ=255) tGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGgcttccagcgcttccag > 1:2578158/1‑69 (MQ=255) tGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGgcttccagcgcttccag > 1:2778019/1‑69 (MQ=255) tGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGgcttccagcgcttccag < 1:1293457/69‑1 (MQ=255) | CCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGGCTTCCAGCGCTTCCAG > minE/1581503‑1581632 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |