| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,581,666 | G→A | 100% | A523A (GCC→GCT) | ligA ← | DNA ligase, NAD(+)‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,581,666 | 0 | G | A | 84.8% | 40.0 / 0.2 | 26 | A523A (GCC→GCT) | ligA | DNA ligase, NAD(+)‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (0/4); new base A (0/22); total (0/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGCGGCTTCCAGCGCTTCCAGCGTGCCGAAATATGCCGCCAGACCTGCTGCGGTGGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGAGCAAAGGT > minE/1581612‑1581716 | aGCGGCTTCCAGCGCTTCCAGCGTGCCGAAATATGCCGCCAGACCTGCTGCGGTGGCCTCGCCGACtt < 1:1380634/68‑1 (MQ=255)aGCGGCTTCCAGCGCTTCCAGCGTGCCGAAATATGCCGCCAGACCTGCTGCGGTGGCCTCGCCGACtt < 1:2896921/68‑1 (MQ=255) cgcttccagcgTGCCGAAATATGCCGCCAGACCTGCTGCGGTGGCCTCGCCGACTTCACGGATGCCAAg < 1:709063/69‑1 (MQ=255) ttccagcgTGCCGAAATATGCCGCCAGACCTGCTGCGGTGGCCTCGCCGACTTCACGGATGCCAAGTg < 1:658826/68‑1 (MQ=255) ccgccAGACCT‑CTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:2722038/69‑1 (MQ=255) gccAGACCTGCTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:926913/68‑1 (MQ=255) gccAGACCTGCTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:1059227/68‑1 (MQ=255) gccAGACCTGCTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:905658/68‑1 (MQ=255) gccAGACCTGCTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:627407/68‑1 (MQ=255) gccAGACCTGCTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:534656/68‑1 (MQ=255) gccAGACCTGCTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:430054/68‑1 (MQ=255) gccAGACCTGCTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:369726/68‑1 (MQ=255) gccAGACCTGCTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:3277937/68‑1 (MQ=255) gccAGACCTGCTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:3156848/68‑1 (MQ=255) gccAGACCTGCTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:3059079/68‑1 (MQ=255) gccAGACCTGCTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:2701767/68‑1 (MQ=255) gccAGACCTGCTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:2647867/68‑1 (MQ=255) gccAGACCTGCTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:2221721/68‑1 (MQ=255) gccAGACCTGCTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:2129867/68‑1 (MQ=255) gccAGACCTGCTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:2022302/68‑1 (MQ=255) gccAGACCTGCTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:1884441/68‑1 (MQ=255) gccAGACCTGCTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:1866148/68‑1 (MQ=255) gccAGACCTGCTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:1446427/68‑1 (MQ=255) gccAGACCTGCTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:1324574/68‑1 (MQ=255) gccAGACCTGCTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:1142545/68‑1 (MQ=255) gccAGACCTGCTGCGGTAGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGGGCAAAggt < 1:113413/68‑1 (MQ=255) | AGCGGCTTCCAGCGCTTCCAGCGTGCCGAAATATGCCGCCAGACCTGCTGCGGTGGCCTCGCCGACTTCACGGATGCCAAGTGCATAGAGGAAGCGAGCAAAGGT > minE/1581612‑1581716 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |