| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,582,181 | G→A | 30.5% | L352L (CTG→TTG) | ligA ← | DNA ligase, NAD(+)‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,582,181 | 0 | G | A | 30.5% | 74.4 / 34.6 | 79 | L352L (CTG→TTG) | ligA | DNA ligase, NAD(+)‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (13/41); new base A (22/2); total (35/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.74e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTA > minE/1582113‑1582236 | tttATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAg < 1:2438728/69‑1 (MQ=255)tttATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAg < 1:2085606/69‑1 (MQ=255)tttATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAg < 1:2073525/69‑1 (MQ=255)tttATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAg < 1:618139/69‑1 (MQ=255)tttATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAg < 1:792652/69‑1 (MQ=255)tttATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAg < 1:1791184/69‑1 (MQ=255) gCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGcc < 1:2775234/69‑1 (MQ=255) gCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGcc < 1:2828017/69‑1 (MQ=255) gCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGcc < 1:726617/69‑1 (MQ=255) gCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGcc < 1:153746/69‑1 (MQ=255) gCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGcc < 1:628998/69‑1 (MQ=255) ccAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGcc < 1:1035302/68‑1 (MQ=255) cAATGCGTAAACCAAGACGTTCGATTTCATCGGCATTGTGTAAGGTTGCGTTACTCACCAGCACGcg < 1:603487/67‑2 (MQ=255) cGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAg < 1:3157884/56‑1 (MQ=255) cGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAg < 1:352885/56‑1 (MQ=255) cGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAg < 1:335789/56‑1 (MQ=255) cGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAg < 1:1737280/56‑1 (MQ=255) cGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAg < 1:196849/56‑1 (MQ=255) cGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAg < 1:2398773/56‑1 (MQ=255) cGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAg < 1:398976/56‑1 (MQ=255) cGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAg < 1:1131517/56‑1 (MQ=255) cGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAg < 1:1104944/56‑1 (MQ=255) cGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACa > 1:1417297/1‑69 (MQ=255) cGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACa > 1:2167466/1‑69 (MQ=255) cGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGAAACa > 1:1001810/1‑69 (MQ=255) gTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGcc < 1:971103/61‑1 (MQ=255) tAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAg < 1:2500205/54‑1 (MQ=255) tCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAAc < 1:1013036/68‑1 (MQ=255) tCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAAc < 1:2400471/68‑1 (MQ=255) tCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAAc < 1:2722131/68‑1 (MQ=255) tCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAAc < 1:3082744/68‑1 (MQ=255) tCCGCATTGTGTAAGGTTGCGATACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAAc < 1:637642/68‑1 (MQ=255) cGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGtt < 1:1128290/53‑1 (MQ=255) cGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGtt < 1:900523/53‑1 (MQ=255) cGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGtt < 1:129965/53‑1 (MQ=255) cGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGtt < 1:3379230/53‑1 (MQ=255) cGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGtt < 1:1821364/53‑1 (MQ=255) cGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGtt < 1:2305688/53‑1 (MQ=255) cGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGtt < 1:2359583/53‑1 (MQ=255) cGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGtt < 1:1640569/53‑1 (MQ=255) cGCATTGTGTAAGGTTGCGTTACACACCAGCACGCCTGCAACATGGACAGGtt < 1:994402/53‑1 (MQ=255) gCATTGTGTAAGGTTGCGTTACTCACCATCACGCCTGCAACATGGACAGGtt < 1:1549934/52‑1 (MQ=255) gCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGtt < 1:379608/52‑1 (MQ=255) ttGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTa < 1:146472/69‑1 (MQ=255) ttGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTa < 1:440979/69‑1 (MQ=255) ttGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTa < 1:678785/69‑1 (MQ=255) ttGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTa < 1:3181124/69‑1 (MQ=255) ttGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTa < 1:2548250/69‑1 (MQ=255) ttGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTa < 1:3021579/69‑1 (MQ=255) ttGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTa < 1:2619999/69‑1 (MQ=255) gTTGCGTTACTCACCAGCACGCCTGTAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGcc > 1:3012179/1‑68 (MQ=255) gTTGCGTTACTCACCAGCACGCCTGCAACATGGACAgg > 1:2573754/1‑38 (MQ=255) gTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGcc > 1:2260235/1‑68 (MQ=255) gTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGcc > 1:2730428/1‑68 (MQ=255) gTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGcc > 1:974907/1‑68 (MQ=255) gTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGcc > 1:1441110/1‑68 (MQ=255) gTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGcc > 1:2831898/1‑68 (MQ=255) gTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGcc > 1:1051513/1‑68 (MQ=255) gTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGcc > 1:2523739/1‑68 (MQ=255) gTTGAGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGcc > 1:1877196/1‑68 (MQ=255) gCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTa < 1:1019647/69‑1 (MQ=255) gCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTa < 1:933728/69‑1 (MQ=255) gCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTa < 1:438976/69‑1 (MQ=255) gCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTa < 1:2492369/69‑1 (MQ=255) gCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTa < 1:3414893/69‑1 (MQ=255) gCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTa < 1:2385946/69‑1 (MQ=255) gCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTa < 1:3250951/69‑1 (MQ=255) gCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTa < 1:13931/69‑1 (MQ=255) gCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTa < 1:2984594/69‑1 (MQ=255) gCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTa < 1:2872905/69‑1 (MQ=255) gCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTa < 1:2138876/69‑1 (MQ=255) tACTCACCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTaa < 1:2302127/54‑1 (MQ=255) tACTCACCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTaa < 1:2769330/54‑1 (MQ=255) aCCAACACGCCAGCATCATGGACAGGTTCCAGACGCGCAACAGGc > 1:2202732/1‑45 (MQ=255) aCCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCaa > 1:833751/1‑40 (MQ=38) aCCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAgga > 1:402630/1‑44 (MQ=255) aCCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAGGc > 1:78043/1‑45 (MQ=255) aCCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAGGc > 1:1884844/1‑45 (MQ=255) aCCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAGGc > 1:1408241/1‑45 (MQ=255) aCCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAGGc > 1:681775/1‑45 (MQ=255) aCCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAGGc > 1:1052437/1‑45 (MQ=255) aCCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAGGc > 1:1543638/1‑45 (MQ=255) aCCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAGGc > 1:552911/1‑45 (MQ=255) aCCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAGGc > 1:495960/1‑45 (MQ=255) aCCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAGGc > 1:1644792/1‑45 (MQ=255) aCCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAGGc > 1:390327/1‑45 (MQ=255) aCCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAGGc > 1:1768031/1‑45 (MQ=255) aCCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAGGc > 1:3231059/1‑45 (MQ=255) aCCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAGGc > 1:1897208/1‑45 (MQ=255) aCCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAGGc > 1:2026947/1‑45 (MQ=255) aCCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAGGc > 1:3008506/1‑45 (MQ=255) aCCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAGGc > 1:2690425/1‑45 (MQ=255) aCCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAGGc > 1:2485480/1‑45 (MQ=255) aCCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAGGc > 1:2363223/1‑45 (MQ=255) aCCAACACGCCAGCAACATGGACAGGTTCCAGACGCGCAACAGGc > 1:2424496/1‑45 (MQ=255) | TTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTA > minE/1582113‑1582236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |