| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,582,830 | G→C | 40.9% | T135T (ACC→ACG) | ligA ← | DNA ligase, NAD(+)‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,582,830 | 0 | G | C | 40.9% | 31.8 / 64.5 | 66 | T135T (ACC→ACG) | ligA | DNA ligase, NAD(+)‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (27/12); new base C (15/12); total (42/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
ATGGCGCGAATAGTACGCACATTAGACGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGC > minE/1582771‑1582890 | aTGGCGCGAATAGTACGCACATTAGACGTAATATCTTCCCCGGTGGTGCCATCGCCACGCGTCGCGGCa < 1:66529/69‑1 (MQ=255)aTGGCGCGAATAGTACGCACATTAGACGTAATATCTTCCCCGGTGGTGCCATCGCCACGCGTCGCGGCa < 1:64265/69‑1 (MQ=255)aTGGCGCGAATAGTACGCACATTAGACGTAATATCTTCCCCGGTGGTGCCATCGCCACGCGTCGCGGCa < 1:638846/69‑1 (MQ=255)aTGGCGCGAATAGTACGCACATTAGACGTAATATCTTCCCCGGTGGTGCCATCGCCACGCGTCGCGGCa < 1:2176737/69‑1 (MQ=255)aTGGCGCGAATAGTACGCACATTAGACGTAATATCTTCCCCGGTGGTGCCATCGCCACGCGTCGCGGCa < 1:216592/69‑1 (MQ=255)aTGGCGCGAATAGTACGCACATTAGACGTAATATCTTCCCCGGTGGTGCCATCGCCACGCGTCGCGGCa < 1:2152940/69‑1 (MQ=255)aTGGCGCGAATAGTACGCACATTAGACGTAATATCTTCCCCGGTGGTGCCATCGCCACGCGTCGCGGCa < 1:1980104/69‑1 (MQ=255)aTGGCGCGAATAGTACGCACATTAGACGTAATATCTTCCCCGGTGGTGCCATCGCCACGCGTCGCGGCa < 1:3426917/69‑1 (MQ=255)aTGGCGCGAATAGTACGCACATTAGACGTAATATCTTCCCCGGTGGTGCCATCGCCACGCGTCGCGGCa < 1:1912434/69‑1 (MQ=255)aTGGCGCGAATAGTACGCACATTAGACGTAATATCTTCCCCGGTGGTGCCATCGCCACGCGTCGCGGCa < 1:3151415/69‑1 (MQ=255)aTGGCGCGAATAGTACGCACATTAGACGTAATATCTTCCCCGGTGGTGCCATCGCCACGCGTCGCGGCa < 1:1672/69‑1 (MQ=255) gcgcGAATAGTACGCACATTAGACGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTg < 1:2351466/69‑1 (MQ=255) gcgcGAATAGTACGCACATTAGACGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTg < 1:1784621/69‑1 (MQ=255) gcgcGAATAGTACGCACATTAGACGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTg < 1:1501187/69‑1 (MQ=255) gcgcGAATAGTACGCACATTAGACGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTg < 1:1254119/69‑1 (MQ=255) gcgcGAATAGTACGCACATTAGACGTGATATCTTCCCCGGTGGTGACATCGCCACGGGTCGCGGCACTg < 1:464270/69‑1 (MQ=255) aGTACGCACATTAGACGTAATATCTTCCCCGGTGGTGCCATCGCCACGCGTCGCGGCa < 1:2837770/58‑1 (MQ=255) aTTAGACGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTg < 1:1181614/52‑1 (MQ=255) cGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAg < 1:245243/69‑1 (MQ=255) cGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAg < 1:2920908/69‑1 (MQ=255) atatCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATa > 1:2666041/1‑69 (MQ=255) atatCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATa > 1:1431924/1‑69 (MQ=255) atatCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATa > 1:2321115/1‑69 (MQ=255) gtgCCATCGCCACGCGTCGCGGCACTGACTGAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:2052309/1‑69 (MQ=255) gtgCCATCGCCACGCGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTg > 1:2505117/1‑57 (MQ=255) gtgCCATCGCCACGCGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCaa > 1:3361556/1‑64 (MQ=255) gtgCCATCGCCACGCGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:2428719/1‑69 (MQ=255) gtgCCATCGCCACGCGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:1025868/1‑69 (MQ=255) gtgCCATCGCCACGCGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:2745757/1‑69 (MQ=255) gtgCCATCGCCACGCGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:3065531/1‑69 (MQ=255) gtgCCATCGCCACGCGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:3194908/1‑69 (MQ=255) gtgCCATCGCCACGCGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:3201362/1‑69 (MQ=255) gtgCCATCGCCACGCGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:3286332/1‑69 (MQ=255) gtgCCATCGCCACGCGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:3430094/1‑69 (MQ=255) gtgCCATCGCCACGCGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:2427106/1‑69 (MQ=255) gtgCCATCGCCACGCGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:1724333/1‑69 (MQ=255) gtgCCATCGCCACGCGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:100997/1‑69 (MQ=255) gtgCCATCGCCACGCGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:2222910/1‑69 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATGCAGAATACTGACGGCAAGACCa > 1:1523961/1‑67 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAg < 1:1599731/48‑1 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAg > 1:1772664/1‑48 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAg > 1:3361236/1‑48 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAg < 1:1383878/48‑1 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAg < 1:1327514/48‑1 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAg < 1:682526/48‑1 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAg > 1:2377276/1‑48 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:1174080/1‑67 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:3326762/1‑67 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:3314203/1‑67 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:851031/1‑67 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:963683/1‑67 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:1654210/1‑67 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:3160478/1‑67 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:1990330/1‑67 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:2831245/1‑67 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:2818043/1‑67 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:2568902/1‑67 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:2491374/1‑67 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCa > 1:2398192/1‑67 (MQ=255) gCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGAACa > 1:1079094/1‑67 (MQ=255) gCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGc > 1:1751583/1‑67 (MQ=255) gCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGc > 1:1304398/1‑67 (MQ=255) gCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGc > 1:2737679/1‑67 (MQ=255) gCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGc > 1:107048/1‑67 (MQ=255) gCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGc > 1:2340336/1‑67 (MQ=255) gCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGc > 1:674260/1‑67 (MQ=255) | ATGGCGCGAATAGTACGCACATTAGACGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGC > minE/1582771‑1582890 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |