| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,583,611 | A→G | 37.5% | N227N (AAT→AAC) | zipA ← | cell division protein involved in Z ring assembly |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,583,611 | 0 | A | G | 37.5% | 22.8 / 28.4 | 48 | N227N (AAT→AAC) | zipA | cell division protein involved in Z ring assembly |
| Reads supporting (aligned to +/- strand): ref base A (14/16); new base G (12/6); total (26/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
TTCGCCAGGCTGAATAACGCCGGGCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGC > minE/1583552‑1583678 | ttCGCCAGGCTGAATAACGCCGGGCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATc > 1:1529589/1‑68 (MQ=255) cTGAATAACGCCGGGCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATGTTCATATCGCCAAAAATg < 1:1767562/69‑1 (MQ=255) cTGAATAACGCCGGGCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATGTTCATATCGCCAAAAATg < 1:2940449/69‑1 (MQ=255) cTGAATAACGCCGGGCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATGTTCATATCGCCAAAAATg < 1:588625/69‑1 (MQ=255) cTGAATAACGCCGGGCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATGTTCATATCGCCAAAAATg < 1:3243703/69‑1 (MQ=255) tGAATAACGCCGGTCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATGTTCATATCGCCAAAAATg < 1:669618/68‑1 (MQ=255) aCGCCGGGCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCg > 1:2515927/1‑69 (MQ=255) aCGCCGGGCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCg > 1:1577642/1‑69 (MQ=255) aCGCCGGGCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCg > 1:492515/1‑69 (MQ=255) aCGCCGGGCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCg > 1:557448/1‑69 (MQ=255) aCGCCGGGCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCg > 1:1028696/1‑69 (MQ=255) gCCGCTGCCATCCGGGCTAAGATGACGATTGTAAATATTCATATCGCCAAAAATGAAGCCCGCttgttg < 1:874507/69‑1 (MQ=255) gCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCttgttg < 1:3403378/69‑1 (MQ=255) gCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCttgttg < 1:317948/69‑1 (MQ=255) gCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCttgttg < 1:502417/69‑1 (MQ=255) gCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCttgttg < 1:2932753/69‑1 (MQ=255) gCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCttgttg < 1:2881254/69‑1 (MQ=255) gCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCttgttg < 1:2790211/69‑1 (MQ=255) gCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCttgttg < 1:106613/69‑1 (MQ=255) gCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCttgttg < 1:1921345/69‑1 (MQ=255) gCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCttgttg < 1:1267681/69‑1 (MQ=255) gCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCttgttg < 1:1347869/69‑1 (MQ=255) gCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCttgttg < 1:1356792/69‑1 (MQ=255) ccGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTAATATCGCCAAAAATGAAGCCCGCttgttg < 1:435088/68‑1 (MQ=255) tGCCATCCGGGCTAAGATGACGATGGTAAATGTTCATATCGCCAAAAATg < 1:1101560/50‑1 (MQ=255) gCCATCCGGGCTAAGATGACGCTGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGc > 1:1848709/1‑68 (MQ=255) gCCATCCGGGCTAAGATGACGATGGTAAATGTTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCt > 1:3416815/1‑69 (MQ=255) gCCATCCGGGCTAAGATGACGATGGTAAATGTTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCt > 1:1430450/1‑69 (MQ=255) gCCATCCGGGCTAAGATGACGATGGTAAATGTTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCt > 1:3201964/1‑69 (MQ=255) gCCATCCGGGCTAAGATGACGATGGTAAATGTTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCt > 1:1442941/1‑69 (MQ=255) gCCATCCGGGCTAAGATGACGATGGTAAATGTTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCt > 1:3033935/1‑69 (MQ=255) gCCATCCGGGCTAAGATGACGATGGTAAATGTTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCt > 1:1374417/1‑69 (MQ=255) gCCATCCGGGCTAAGATGACGATGGTAAATGTTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCt > 1:604318/1‑69 (MQ=255) gCCATCCGGGCTAAGATGACGATGGTAAATGTTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCt > 1:2560486/1‑69 (MQ=255) gCCATCCGGGCTAAGATGACGATGGTAAATGTTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCt > 1:2375449/1‑69 (MQ=255) gCCATCCGGGCTAAGATGACGATGGTAAATGTTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCt > 1:2293709/1‑69 (MQ=255) gCCATCCGGGCTAAGATGACGATGGTAAATGTTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCt > 1:2284586/1‑69 (MQ=255) gCCATCCGGGCTAAGATGACGATGGTAAATGTTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCt > 1:2156089/1‑69 (MQ=255) gCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGc > 1:1369441/1‑68 (MQ=255) gCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGc > 1:890560/1‑68 (MQ=255) gCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGc > 1:425904/1‑68 (MQ=255) gCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGc > 1:14240/1‑68 (MQ=255) gCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGc > 1:1855128/1‑68 (MQ=255) gCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGc > 1:2841748/1‑68 (MQ=255) gCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGc > 1:2180994/1‑68 (MQ=255) aTGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGt < 1:1668142/67‑1 (MQ=255) aCGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAg < 1:2988645/63‑1 (MQ=255) tAAATATTCATATCGCCAAAAATGAAGCCCGCttgttg < 1:2301162/38‑1 (MQ=255) tatTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGc < 1:2829743/69‑1 (MQ=255) tatTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGc < 1:1912047/69‑1 (MQ=255) | TTCGCCAGGCTGAATAACGCCGGGCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGC > minE/1583552‑1583678 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |