Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 21,484 A→G 54.1% V472V (GTA→GTG ileS → isoleucyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE21,4840AG54.1% 27.3 / 124.1 146V472V (GTA→GTGileSisoleucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (20/47);  new base G (47/32);  total (67/79)
Fisher's exact test for biased strand distribution p-value = 4.44e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 148 total aligned reads displayed.

GCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAG  >  minE/21418‑21551
                                                                  |                                                                   
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:1324655/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:1802046/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:1817947/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:2912528/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:1675637/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:1840875/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:1886328/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:2567171/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:3202012/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:249041/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:3428798/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:2409932/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:590913/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:817050/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:871391/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:928334/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:21258/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:1159921/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:2234142/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:1143012/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:1104551/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:1086622/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:1046239/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:2224736/69‑1 (MQ=38)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTAcc                                                                   <  1:1630927/69‑1 (MQ=255)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTAcc                                                                   <  1:277849/69‑1 (MQ=255)
gcgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTAcc                                                                   <  1:2899545/69‑1 (MQ=255)
     tCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGt                                                              <  1:2818039/69‑1 (MQ=255)
     tCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGt                                                              <  1:3089452/69‑1 (MQ=255)
       gAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGcc                                                                   <  1:1875960/62‑1 (MQ=37)
       gAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTc                                                             >  1:1445910/1‑68 (MQ=255)
       gAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCa                                                            >  1:2966220/1‑69 (MQ=255)
       gAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCa                                                            >  1:1157099/1‑69 (MQ=255)
         gTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCAc                                                           <  1:987523/68‑1 (MQ=255)
         gTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCAc                                                           <  1:2783944/68‑1 (MQ=255)
         gTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCAc                                                           <  1:277016/68‑1 (MQ=255)
         gTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCAc                                                           <  1:3377562/68‑1 (MQ=255)
         gTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCAc                                                           <  1:426680/68‑1 (MQ=255)
         gTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCAc                                                           <  1:959689/68‑1 (MQ=255)
         gTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCAc                                                           <  1:2369488/68‑1 (MQ=255)
         gTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCAc                                                           <  1:1022809/68‑1 (MQ=255)
                   gCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGCCGATGTCACTGTTCGTGca                                                 >  1:730264/1‑68 (MQ=38)
                   gCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGCCGATGTCACTGTTCGTGca                                                 >  1:2910066/1‑68 (MQ=38)
                   gCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGCCGATGTCACTGTTCGTGca                                                 >  1:1297247/1‑68 (MQ=38)
                   gCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGCCGATGTCACTGTTCGTGca                                                 >  1:3102914/1‑68 (MQ=38)
                   gCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGCCGATGTCACTGTTCGTGca                                                 >  1:2703630/1‑68 (MQ=38)
                   gCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGCCGATGTCACTGTTCGTGca                                                 >  1:2072655/1‑68 (MQ=38)
                   gCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGCCGATGTCACTGTTCGTGca                                                 >  1:2494989/1‑68 (MQ=38)
                   gCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGCCGATGTCACTGTTCGTGca                                                 >  1:3160688/1‑68 (MQ=38)
                   gCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGCCGATGTCACTGTTCGTGca                                                 >  1:1440830/1‑68 (MQ=38)
                   gCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGCCGATGTCACTGTTCGTGca                                                 >  1:2408248/1‑68 (MQ=38)
                   gCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGCCGATGTCACTGTTCGTGca                                                 >  1:743010/1‑68 (MQ=38)
                   gCTAACCGTCCTGACTGGTGTATCTCTCGTCAGCGTACCTGGGGCGTGCCGATGTCACTGTTCGTGca                                                 >  1:1437593/1‑68 (MQ=38)
                   gCTAACCGTCCTGACTGGTGTATCTCTCCTCAGCGTACCTGGGGCGTGCCGATGTCACTGTTCGTGc                                                  >  1:2661162/1‑67 (MQ=255)
                      aaCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACaaa                                             <  1:1163101/69‑1 (MQ=255)
                             cTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCAc                                                           <  1:1926143/48‑1 (MQ=255)
                                         tctcCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATc                          >  1:2161995/1‑69 (MQ=255)
                                         tctcCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATc                          >  1:2339801/1‑69 (MQ=255)
                                         tctcCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATc                          >  1:1753556/1‑69 (MQ=255)
                                                      cACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTg              <  1:2429531/68‑1 (MQ=255)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGa                                           >  1:2535682/1‑37 (MQ=38)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGc                             >  1:494775/1‑51 (MQ=255)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGt                     >  1:3230271/1‑59 (MQ=255)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTa                    >  1:2996985/1‑60 (MQ=255)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc                   >  1:1688707/1‑61 (MQ=255)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc                   >  1:595943/1‑61 (MQ=255)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc                   >  1:430782/1‑61 (MQ=255)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc                   >  1:1350445/1‑61 (MQ=255)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc                   >  1:399324/1‑61 (MQ=255)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc                   >  1:2279261/1‑61 (MQ=255)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc                   >  1:336470/1‑61 (MQ=255)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc                   >  1:3348440/1‑61 (MQ=255)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc                   >  1:2156136/1‑61 (MQ=255)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc                   >  1:813406/1‑61 (MQ=255)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc                   >  1:2122357/1‑61 (MQ=255)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc                   >  1:2452393/1‑61 (MQ=255)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc                   >  1:2103476/1‑61 (MQ=255)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc                   >  1:1253571/1‑61 (MQ=255)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc                   >  1:1594664/1‑61 (MQ=255)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc                   >  1:516987/1‑61 (MQ=255)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc                   >  1:86549/1‑61 (MQ=255)
                                                        ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc                   >  1:1769936/1‑61 (MQ=255)
                                                         cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt          <  1:3168679/69‑1 (MQ=255)
                                                         cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt          <  1:1302675/69‑1 (MQ=255)
                                                         cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt          <  1:1283538/69‑1 (MQ=255)
                                                         cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt          <  1:1034988/69‑1 (MQ=255)
                                                         cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt          <  1:3254784/69‑1 (MQ=255)
                                                         cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt          <  1:1672705/69‑1 (MQ=255)
                                                         cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt          <  1:2948627/69‑1 (MQ=255)
                                                         cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt          <  1:1914394/69‑1 (MQ=255)
                                                          tGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTg         <  1:630574/69‑1 (MQ=255)
                                                             ggTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTg         <  1:463372/66‑1 (MQ=255)
                                                               tgtACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGAt       >  1:2726320/1‑66 (MQ=255)
                                                                 tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaag  <  1:533156/69‑1 (MQ=255)
                                                                 tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaag  <  1:2264271/69‑1 (MQ=255)
                                                                 tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGga    >  1:651498/1‑67 (MQ=255)
                                                                 tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGga    >  1:1392556/1‑67 (MQ=255)
                                                                 tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGga    >  1:3314107/1‑67 (MQ=255)
                                                                 tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGga    >  1:918846/1‑67 (MQ=255)
                                                                 tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGga    >  1:2048193/1‑67 (MQ=255)
                                                                  |                                                                   
GCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAG  >  minE/21418‑21551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: