| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,585,072 | C→T | 35.1% | C184C (TGC→TGT) | cysZ → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,585,072 | 0 | C | T | 35.1% | 94.3 / 71.7 | 125 | C184C (TGC→TGT) | cysZ | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (66/15); new base T (15/29); total (81/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.88e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.30e-01 | |||||||||||
Only 100 of 125 total aligned reads displayed.
TTGGGCAAACCGTCGCGCCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAA > minE/1585005‑1585138 | ttGGGCAAACCGTCGCGCCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCg > 1:1131003/1‑69 (MQ=255)ttGGGCAAACCGTCGCGCCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCg > 1:1641926/1‑69 (MQ=255) gggCAAACCGTCGCGCCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGAt > 1:1588741/1‑69 (MQ=255) gggCAAACCGTCGCGCCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGAt > 1:940042/1‑69 (MQ=255) ccGTCGCGCCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTAcccc > 1:3308598/1‑68 (MQ=255) ccGTCGCGCCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCt > 1:2176217/1‑69 (MQ=255) ccGTCGCGCCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCt > 1:3322329/1‑69 (MQ=255) ccGTCGCGCCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCt > 1:318452/1‑69 (MQ=255) ccGTCGCGCCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCt > 1:1453341/1‑69 (MQ=255) ccGTCGCGCCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCt > 1:3036939/1‑69 (MQ=255) ccGTCGCGCCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCt > 1:2017845/1‑69 (MQ=255) gcgcCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGAt > 1:2364835/1‑69 (MQ=255) gcgcCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGAt > 1:2336302/1‑69 (MQ=255) gcgcCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGAt > 1:1350672/1‑69 (MQ=255) gcgcCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGAt > 1:1641430/1‑69 (MQ=255) gcgcCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGAt > 1:3208341/1‑69 (MQ=255) gcgcCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGAt > 1:1478629/1‑69 (MQ=255) gcgcCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGAt > 1:1047002/1‑69 (MQ=255) aCTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACaa > 1:2742857/1‑69 (MQ=255) gtgGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAAcc > 1:1692855/1‑62 (MQ=255) gtgGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGt > 1:1843183/1‑69 (MQ=255) gtgGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGt > 1:3299681/1‑69 (MQ=255) gtgGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGt > 1:1784720/1‑69 (MQ=255) gtgGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGt > 1:178856/1‑69 (MQ=255) gtgGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGt > 1:399581/1‑69 (MQ=255) gtgGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGt > 1:425672/1‑69 (MQ=255) gtgGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGt > 1:1157959/1‑69 (MQ=255) gtgGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGt > 1:2875304/1‑69 (MQ=255) gtgGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGt > 1:1137968/1‑69 (MQ=255) gtgGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGt > 1:2405497/1‑69 (MQ=255) gtgGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGt > 1:2461547/1‑69 (MQ=255) gtgGTTCCTGTTTAGCGCCTGGATGTTAGCAATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGt > 1:2161029/1‑69 (MQ=255) ttgTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTg > 1:1518863/3‑69 (MQ=255) tgGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTg > 1:3098977/1‑69 (MQ=255) tgGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTg > 1:3026401/1‑69 (MQ=255) tgGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTg > 1:2658212/1‑69 (MQ=255) tgGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTg > 1:1019707/1‑69 (MQ=255) tgGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTg > 1:537915/1‑69 (MQ=255) tgGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTg > 1:118854/1‑69 (MQ=255) tgGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTg > 1:1132144/1‑69 (MQ=255) ccTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTa > 1:199648/1‑42 (MQ=255) ccTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGt > 1:1143951/1‑68 (MQ=255) ccTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGt > 1:1965072/1‑68 (MQ=255) ccTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGt > 1:2895140/1‑68 (MQ=255) ccTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCAATTACCCCTTCGATAACCACAAAGTGCCGt > 1:44354/1‑68 (MQ=255) gTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGtt > 1:2075557/1‑66 (MQ=255) gTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGtt > 1:2661034/1‑66 (MQ=255) atgtAGCGCCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:1065851/66‑1 (MQ=255) tAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTa > 1:2765050/1‑65 (MQ=255) tAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTa > 1:2316069/1‑65 (MQ=255) tAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTa > 1:783434/1‑65 (MQ=255) tAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTa > 1:1987707/1‑65 (MQ=255) gcCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:967360/62‑1 (MQ=255) gcCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:282980/62‑1 (MQ=255) gcCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:862977/62‑1 (MQ=255) gcCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:3003015/62‑1 (MQ=255) gcCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:655109/62‑1 (MQ=255) gcCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:556689/62‑1 (MQ=255) gcCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:520498/62‑1 (MQ=255) gcCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:330787/62‑1 (MQ=255) gcCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:3246443/62‑1 (MQ=255) gcCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:1771730/62‑1 (MQ=255) gcCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:1030783/62‑1 (MQ=255) gcCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:116935/62‑1 (MQ=255) gcCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:1267226/62‑1 (MQ=255) gcCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:1373899/62‑1 (MQ=255) gcCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:163215/62‑1 (MQ=255) gcCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:2089010/62‑1 (MQ=255) gcCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:2130346/62‑1 (MQ=255) gcCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:2174583/62‑1 (MQ=255) gcCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:2542051/62‑1 (MQ=255) gcCTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:2535825/62‑1 (MQ=255) ccTGGATGTTAGCCATCCAGTATTGTGATTACCCCTTCGATAACCACAAAGTGCCGTTTaa < 1:2622875/61‑1 (MQ=255) tGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTAAAGAGATgcgc < 1:1821668/69‑1 (MQ=255) ttAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTAAAGAGATGCGCACCGcc > 1:3243980/1‑69 (MQ=255) aGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTa < 1:1618082/50‑1 (MQ=255) aGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTa < 1:1114475/50‑1 (MQ=255) aGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTa < 1:90966/50‑1 (MQ=255) aGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTa < 1:2692772/50‑1 (MQ=255) aGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTa > 1:817099/1‑50 (MQ=255) aGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTa > 1:2541770/1‑50 (MQ=255) aGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTa < 1:1364463/50‑1 (MQ=255) aGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTa > 1:1556956/1‑50 (MQ=255) aGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTa < 1:2795921/50‑1 (MQ=255) aGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTa > 1:2288402/1‑50 (MQ=255) aGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTa > 1:2371761/1‑50 (MQ=255) aGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTa < 1:2381673/50‑1 (MQ=255) aGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTa < 1:3054943/50‑1 (MQ=255) aGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTa < 1:2099698/50‑1 (MQ=255) aGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTa < 1:2096533/50‑1 (MQ=255) aGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTa > 1:2810363/1‑50 (MQ=255) aGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTa < 1:1618726/50‑1 (MQ=255) gCGATTACCCCTTCGATAACCACAAAGTGCCGTTTAAAGAGATGCGCa > 1:1453427/1‑48 (MQ=255) gCGATTACCCCTTCGATAACCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTg > 1:1438735/1‑56 (MQ=255) gCGATTACCCCTTCGATAACCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCaaa > 1:1618305/1‑68 (MQ=255) gCGATTACCCCTTCGATAACCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCaaa > 1:706479/1‑68 (MQ=255) gCGATTACCCCTTCGATAACCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCaaa > 1:1566396/1‑68 (MQ=255) gCGATTACCCCTTCGATAACCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCaaa > 1:408740/1‑68 (MQ=255) gCGATTACCCCTTCGATAACCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCaaa > 1:2347853/1‑68 (MQ=255) gCGATTACCCCTTCGATAACCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCaaa > 1:388658/1‑68 (MQ=255) | TTGGGCAAACCGTCGCGCCGGTACTGTGGTTCCTGTTTAGCGCCTGGATGTTAGCCATCCAGTATTGCGATTACCCCTTCGATAACCACAAAGTGCCGTTTAAAGAGATGCGCACCGCCCTGCGCACACGCAAA > minE/1585005‑1585138 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |