| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,590,071 | G→A | 40.8% | G75G (GGC→GGT) | pdxK ← | pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,590,071 | 0 | G | A | 40.8% | 41.1 / 66.5 | 96 | G75G (GGC→GGT) | pdxK | pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase |
| Reads supporting (aligned to +/- strand): ref base G (29/27); new base A (12/27); total (41/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.80e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTC > minE/1590003‑1590134 | cccATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAg > 1:718447/1‑69 (MQ=255) ataGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGc > 1:1308877/1‑68 (MQ=255) aGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTaa > 1:2832334/1‑69 (MQ=255) aGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTaa > 1:693597/1‑69 (MQ=255) gTTACAGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCg > 1:940240/1‑69 (MQ=255) gTTACAGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCg > 1:3307935/1‑69 (MQ=255) gTTACAGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCg > 1:2862595/1‑69 (MQ=255) gTTACAGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCg > 1:1325507/1‑69 (MQ=255) gTTACAGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCg > 1:2679437/1‑69 (MQ=255) gTTACAGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCg > 1:739370/1‑69 (MQ=255) gTTACAGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCg > 1:1157352/1‑69 (MQ=255) gTTACAGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCg > 1:936270/1‑69 (MQ=255) gCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGa < 1:205076/69‑1 (MQ=255) gAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATCACCGCTAAACCATTCATCCGGAATCg < 1:3253561/69‑1 (MQ=255) gAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAACCGCTAAACCATTCATCCGGAATCg < 1:2735139/69‑1 (MQ=255) gAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAACCGCTAAACCATTCATCCGGAATCg < 1:2525872/69‑1 (MQ=255) gAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAACCGCTAAACCATTCATCCGGAATCg < 1:2040955/69‑1 (MQ=255) gAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAACCGCTAAACCATTCATCCGGAATCg < 1:1942535/69‑1 (MQ=255) gAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAACCGCTAAACCATTCATCCGGAATCg < 1:3396005/69‑1 (MQ=255) gAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAACCGCTAAACCATTCATCCGGAATCg < 1:3350807/69‑1 (MQ=255) gAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAACCGCTAAACCATTCATCCGGAATCg < 1:180379/69‑1 (MQ=255) gAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAACCGCTAAACCATTCATCCGGAATCg < 1:3372381/69‑1 (MQ=255) gAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAACCGCTAAACCATTCATCCGGAATCg < 1:411321/69‑1 (MQ=255) gAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAACCGCTAAACCATTCATCCGGAATCg < 1:49836/69‑1 (MQ=255) gAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAACCGCTAAACCATTCATCCGGAATCg < 1:570861/69‑1 (MQ=255) gAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAACCGCTAAACCATTCATCCGGAATCg < 1:1234588/69‑1 (MQ=255) gAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAACCGCTAAACCATTCATCCGGAATCg < 1:1123255/69‑1 (MQ=255) gTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGcac < 1:1974240/69‑1 (MQ=255) ttGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGcac < 1:1286986/68‑1 (MQ=255) ggCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGcc > 1:620128/1‑40 (MQ=255) ggCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGcaccac > 1:3362132/1‑69 (MQ=255) ggCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGcaccac > 1:972221/1‑69 (MQ=255) ggCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGcaccac > 1:451187/1‑69 (MQ=255) ggCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGcaccac > 1:2469435/1‑69 (MQ=255) ggCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGcaccac > 1:2142722/1‑69 (MQ=255) ggCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGcaccac > 1:1624632/1‑69 (MQ=255) ggCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGcacca > 1:2468622/1‑68 (MQ=255) cgcaTCACGCTCCTGAAGCGCACGCAAATATCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGaa < 1:581957/69‑1 (MQ=255) cgcaTCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGaa < 1:3129572/69‑1 (MQ=255) cgcaTCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGaa < 1:3205867/69‑1 (MQ=255) cgcaTCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGaa < 1:545643/69‑1 (MQ=255) cgcaTCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGaa < 1:3248479/69‑1 (MQ=255) cgcaTCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGaa < 1:1936201/69‑1 (MQ=255) cgcaTCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGaa < 1:2565291/69‑1 (MQ=255) gcaTCACGCTCCTGAAGCGCACGCAAATAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGaaa > 1:3007021/1‑69 (MQ=255) gcaTCACGCTCCTGAAGCGCACGCAAATAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGaaa > 1:2220403/1‑69 (MQ=255) gcaTCACGCTCCTGAAGCGCACGCAAATAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGaaa > 1:1805243/1‑69 (MQ=255) gcaTCACGCTCCTGAAGCGCACGCAAATAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGaaa > 1:1489420/1‑69 (MQ=255) gcaTCACGCTCCTGAAGCGCACGCAAATAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGaaa > 1:1347083/1‑69 (MQ=255) gcaTCACGCTCCTGAAGCGCACGCAAATAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGaaa > 1:2374664/1‑69 (MQ=255) gcaTCACGCTCCTGAAGCGCACGCAAATAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGaaa > 1:597825/1‑69 (MQ=255) aTCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAgt < 1:2761192/69‑1 (MQ=255) tCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAgt < 1:2858801/68‑1 (MQ=255) gCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCAt < 1:524024/69‑1 (MQ=255) gCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCAt < 1:3027338/69‑1 (MQ=255) gCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCAt < 1:2314038/69‑1 (MQ=255) gCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCAt < 1:2281472/69‑1 (MQ=255) gCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCAt < 1:2303401/69‑1 (MQ=255) tCCTGAAGCTCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATaa < 1:1125295/69‑1 (MQ=255) tCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATaa < 1:410238/69‑1 (MQ=255) tCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATaa < 1:1752532/69‑1 (MQ=255) tCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATaa < 1:2381838/69‑1 (MQ=255) tCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCAACGTAGAAAGTGTCATaa < 1:2568761/69‑1 (MQ=255) tCCTGAAGCCCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATaa < 1:300790/69‑1 (MQ=255) cTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGt > 1:2806934/1‑53 (MQ=255) cTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATg > 1:1180762/1‑69 (MQ=255) cTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATg > 1:2367478/1‑69 (MQ=255) cTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATg > 1:404913/1‑69 (MQ=255) gAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGca > 1:2883566/1‑44 (MQ=255) gAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAA‑‑GTCATAATgcgg > 1:698913/1‑68 (MQ=255) gAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAA‑‑GTCATAATgcgg > 1:637649/1‑68 (MQ=255) gAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAA‑‑GTCATAATgcgg > 1:3250381/1‑68 (MQ=255) gAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAA‑‑GTCATAATgcgg > 1:1373091/1‑68 (MQ=255) gAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAA‑‑GTCATAATgcag > 1:2163351/1‑66 (MQ=255) gcgcACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATgcggcg < 1:834034/69‑1 (MQ=255) gcgcACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATgcggcg < 1:2985413/69‑1 (MQ=255) gcgcACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATgcggcg < 1:2523878/69‑1 (MQ=255) gcgcACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATgcggcg < 1:406293/69‑1 (MQ=255) gcgcACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATgcggcg < 1:1976209/69‑1 (MQ=255) gcgcACGCAAATAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGAAAGTGTCATAATgcggc < 1:1275348/68‑1 (MQ=255) gcgcACGCAAATAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGAAAGTGTCATAATgcggc < 1:1066073/68‑1 (MQ=255) gcgcACGCAAATAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGAAAGTGTCATAATgcggc < 1:1092916/68‑1 (MQ=255) gcgcACGCAAATAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGAAAGTGTCATAATgcggc < 1:2929788/68‑1 (MQ=255) gcgcACGCAAATAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGAAAGTGTCATAATgcggc < 1:2136067/68‑1 (MQ=255) gcgcACGCAAATAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGAAAGTGTCATAATgcggc < 1:3100644/68‑1 (MQ=255) gcgcACGCAAATAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGAAAGTGTCATAATgcggc < 1:2604187/68‑1 (MQ=255) gcgcACGCAAATAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGAAAGTGTCATAATgcggc < 1:1852060/68‑1 (MQ=255) gcgcACGCAAATAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGAAAGTGTCATAATgcggc < 1:2243778/68‑1 (MQ=255) gcgcACGCAAATAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGAAAGTGTCATAATgcggc < 1:2452382/68‑1 (MQ=255) gcgcACGCAAATAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGAAAGTGTCATAATgcggc < 1:3389179/68‑1 (MQ=255) gcgcACGCAAATAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGAAAGTGTCATAATgcggc < 1:1456990/68‑1 (MQ=255) gcgcACGCAAATAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGAAAGTGTCATAATgcggc < 1:421934/68‑1 (MQ=255) aaaTAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTc > 1:1261198/1‑69 (MQ=255) aaaTAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTc > 1:1227825/1‑69 (MQ=255) aaaTAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTc > 1:3073775/1‑69 (MQ=255) aaaTAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTc > 1:936291/1‑69 (MQ=255) aaaTAACCGCTAAACCATTCATCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTc > 1:251964/1‑69 (MQ=255) | CCCATATAGCCCGTGGTTACAGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTC > minE/1590003‑1590134 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |