| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,590,206 | C→T | 31.8% | V30V (GTG→GTA) | pdxK ← | pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,590,206 | 0 | C | T | 31.8% | 84.7 / 45.6 | 94 | V30V (GTG→GTA) | pdxK | pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase |
| Reads supporting (aligned to +/- strand): ref base C (28/36); new base T (16/14); total (44/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.17e-01 | |||||||||||
CCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATC > minE/1590142‑1590271 | ccGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCAcac > 1:2386681/1‑67 (MQ=255)ccGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGc > 1:505056/1‑69 (MQ=255)ccGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGc > 1:2450316/1‑69 (MQ=255)ccGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGc > 1:1233435/1‑69 (MQ=255)ccGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGc > 1:2442263/1‑69 (MQ=255)ccGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGc > 1:1278425/1‑69 (MQ=255)ccGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGc > 1:3371305/1‑69 (MQ=255)ccGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGc > 1:2279637/1‑69 (MQ=255)ccGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGc > 1:654906/1‑69 (MQ=255)ccGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGc > 1:1939344/1‑69 (MQ=255)ccGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGc > 1:754515/1‑69 (MQ=255)ccGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGAACGGCAATGCTGTTGCCCACGc > 1:664869/1‑69 (MQ=255) ggcacggcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCg < 1:3144646/69‑1 (MQ=255) ggcacggcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCg < 1:3247908/69‑1 (MQ=255) ggcacggcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCg < 1:3217622/69‑1 (MQ=255) ggcacggcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCg < 1:3201491/69‑1 (MQ=255) ggcacggcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCg < 1:3163290/69‑1 (MQ=255) ggcacggcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCg < 1:1014918/69‑1 (MQ=255) ggcacggcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCg < 1:3097843/69‑1 (MQ=255) ggcacggcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCg < 1:3097264/69‑1 (MQ=255) ggcacggcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCg < 1:2310296/69‑1 (MQ=255) ggcacggcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCg < 1:3012700/69‑1 (MQ=255) ggcacggcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCg < 1:1694564/69‑1 (MQ=255) ggcacggcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCg < 1:931595/69‑1 (MQ=255) ggcacggcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCg < 1:1300687/69‑1 (MQ=255) ggcacggcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCg < 1:1700223/69‑1 (MQ=255) gcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAacc < 1:3061734/69‑1 (MQ=255) gcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAacc < 1:2668825/69‑1 (MQ=255) gcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAacc < 1:265972/69‑1 (MQ=255) gcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAacc < 1:1033097/69‑1 (MQ=255) gcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAacc < 1:963677/69‑1 (MQ=255) gcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAacc > 1:2409253/1‑69 (MQ=255) gcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAacc < 1:1241112/69‑1 (MQ=255) gcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAacc < 1:2369322/69‑1 (MQ=255) gcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAacc < 1:2325027/69‑1 (MQ=255) gcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAacc > 1:963185/1‑69 (MQ=255) gcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAacc < 1:1320757/69‑1 (MQ=255) gcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAacc < 1:1986121/69‑1 (MQ=255) gcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAacc < 1:1508296/69‑1 (MQ=255) gcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAacc > 1:721114/1‑69 (MQ=255) gcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAacc < 1:3296714/69‑1 (MQ=255) aCATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGc < 1:1346068/69‑1 (MQ=255) cGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACg > 1:952475/1‑68 (MQ=255) cGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACg > 1:699167/1‑68 (MQ=255) cGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACg > 1:673169/1‑68 (MQ=255) cGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACg > 1:966011/1‑68 (MQ=255) cGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACg > 1:2937041/1‑68 (MQ=255) cGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACg > 1:2792020/1‑68 (MQ=255) cGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACg > 1:348807/1‑68 (MQ=255) cGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACg > 1:1021496/1‑68 (MQ=255) cGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACg > 1:1782557/1‑68 (MQ=255) cGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACg > 1:190322/1‑68 (MQ=255) cGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACg > 1:1776221/1‑68 (MQ=255) cGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACg > 1:2157055/1‑68 (MQ=255) cGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACg > 1:1719880/1‑68 (MQ=255) cGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACg > 1:2565071/1‑68 (MQ=255) cGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACg > 1:1280841/1‑68 (MQ=255) cGTTCTGTTTGATAGCAGGCACCGCAATGCTGTTGCCTACGCTGCCGTACACTACCTGCGACTGCACg > 1:2642903/1‑68 (MQ=255) cTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGt > 1:3033449/1‑44 (MQ=255) cTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTa > 1:2989432/1‑45 (MQ=255) cTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTa > 1:2801947/1‑45 (MQ=255) tGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGatat < 1:590927/69‑1 (MQ=255) ggCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGc < 1:2043364/69‑1 (MQ=255) gCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGc < 1:8485/68‑1 (MQ=255) cAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGccc < 1:1597921/69‑1 (MQ=255) cAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGccc < 1:877964/69‑1 (MQ=255) cAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGccc < 1:342629/69‑1 (MQ=255) cAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGccc < 1:3311843/69‑1 (MQ=255) cAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGccc < 1:1891308/69‑1 (MQ=255) cAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGccc < 1:2599912/69‑1 (MQ=255) cAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGccc < 1:2197535/69‑1 (MQ=255) aaTGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCTGCCTGCAGTGCCCt < 1:2123042/69‑1 (MQ=255) aaTGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGGCCt < 1:1648615/69‑1 (MQ=255) aaTGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCt < 1:3285747/69‑1 (MQ=255) aaTGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCt < 1:2754899/69‑1 (MQ=255) aaTGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCt < 1:282304/69‑1 (MQ=255) aaTGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCt < 1:146644/69‑1 (MQ=255) aaTGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCt < 1:890682/69‑1 (MQ=255) aaTGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCt < 1:2895360/69‑1 (MQ=255) aaTGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCt < 1:532236/69‑1 (MQ=255) aaTGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCt < 1:3393740/69‑1 (MQ=255) aaTGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCt < 1:2705821/69‑1 (MQ=255) aaTGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCt < 1:1879809/69‑1 (MQ=255) aaTGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCACTGCCCt < 1:3076925/69‑1 (MQ=255) cTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTg > 1:1669293/1‑61 (MQ=255) cTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTg > 1:1657116/1‑61 (MQ=255) cTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTg > 1:1522321/1‑61 (MQ=255) cTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTg > 1:182473/1‑61 (MQ=255) gCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGatat > 1:1325834/1‑44 (MQ=255) gCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATc > 1:3042206/1‑69 (MQ=255) gCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATc > 1:1578103/1‑69 (MQ=255) gCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATc > 1:411652/1‑69 (MQ=255) gCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATc > 1:2015601/1‑69 (MQ=255) gCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATc > 1:123720/1‑69 (MQ=255) | CCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATC > minE/1590142‑1590271 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |