Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,600,831 A→C 100% V367V (GTA→GTC)  murP → fused predicted enzyme IIBC components of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,600,8310AC85.8% 25.1 / ‑2.0 14V367V (GTA→GTC) murPfused predicted enzyme IIBC components of PTS
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (5/7);  total (6/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTAC  >  minE/1600803‑1600882
                            |                                                   
aaCCGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTcc                                    <  1:2132299/46‑1 (MQ=255)
aaCCGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTcc                                    <  1:2384637/46‑1 (MQ=255)
aaCCGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTcc                                    <  1:2966939/46‑1 (MQ=255)
aaCCGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTcc                                    <  1:298809/46‑1 (MQ=255)
aaCCGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTcc                                    <  1:628919/46‑1 (MQ=255)
aaCCGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTcc                                    <  1:906550/46‑1 (MQ=255)
aaCCGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTcc                                    <  1:919597/46‑1 (MQ=255)
  ccGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTCCCGGTCTGCTTGGCGTTGGCGAAcc            >  1:2996069/1‑68 (MQ=255)
  ccGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTCCCGGTCTGCTTGGCGTTGGCGAACCg           >  1:2625751/1‑69 (MQ=255)
  ccGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTCCCGGTCTGCTTGGCGTTGGCGAACCg           >  1:3322573/1‑69 (MQ=255)
  ccGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTCCCGGTCTGCTTGGCGTTGGCGAACCg           >  1:382316/1‑69 (MQ=255)
  ccGCACAGTGCATTACGCAGTCAGGTCCGCGGGGCGATTATTCCCGGTCTGCTTGGCGTTGGCGAACCg           >  1:489666/1‑69 (MQ=255)
      acaGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGa       <  1:2061341/69‑1 (MQ=255)
           gcgcTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTAc  >  1:947300/1‑69 (MQ=255)
                            |                                                   
AACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTAC  >  minE/1600803‑1600882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑