Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,600,906	G→A	65.3%	K392K (AAG→AAA)	murP →	fused predicted enzyme IIBC components of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,600,906	0	G	A	65.3%	15.4 / 13.0	30	K392K (AAG→AAA)	murP	fused predicted enzyme IIBC components of PTS
Reads supporting (aligned to +/- strand):  ref base G (3/7);  new base A (9/10);  total (12/18)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

ATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCT  >  minE/1600844‑1600959                                                               |                                                      attCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGttt                                                 <  1:2926911/69‑1 (MQ=255) attCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGttt                                                 <  1:1246139/69‑1 (MQ=255) attCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGttt                                                 <  1:2386176/69‑1 (MQ=255) attCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGttt                                                 <  1:2098376/69‑1 (MQ=255)   tCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTTTCACCCTGCCCCGCATGAAGCCGTTTg                                                <  1:1883781/68‑1 (MQ=255)   tCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTg                                                <  1:1498837/68‑1 (MQ=255)                  cGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAgg                                >  1:954635/1‑69 (MQ=255)                  cGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAgg                                >  1:2749703/1‑69 (MQ=255)                  cGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAgg                                >  1:1984800/1‑69 (MQ=255)                    ttGGCGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAACCGTTTGTTACCGCCTGTTTAggcg                              >  1:206727/1‑69 (MQ=255)                    ttGGCGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAACCGTTTGTTACCGCCTGTTTAggcg                              >  1:2774059/1‑69 (MQ=255)                    ttGGCGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAACCGTTTGTTACCGCCTGTTTAggcg                              >  1:2165436/1‑69 (MQ=255)                    ttGGCGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAACCGTTTGTTACCGCCTGTTTAggcg                              >  1:1766763/1‑69 (MQ=255)                    ttGGCGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAACCGTTTGTTACCGCCTGTTTAggcg                              >  1:711720/1‑69 (MQ=255)                    ttGGCGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAACCGTTTGTTACCGCCTGTTTAggcg                              >  1:1200900/1‑69 (MQ=255)                    ttGGCGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAACCGTTTGTTACCGCCTGTTTAggc                               >  1:653461/1‑68 (MQ=255)                    ttGGCGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAACCGTTTGTTACCGCCTGTTTAggc                               >  1:1751575/1‑68 (MQ=255)                    ttGGCGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAACCGTTTGTTACCGCCTGTTTAggc                               >  1:3129049/1‑68 (MQ=255)                      ggcgAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAACCGTTTGTTACCGCCTGTTTAggc                               <  1:2297761/63‑1 (MQ=255)                      ggcgAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAACCGTTTGTTACCGCCTGTTTAggc                               <  1:407778/63‑1 (MQ=255)                      ggcgAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAACCGTTTGTTACCGCCTGTTTAggc                               <  1:920034/63‑1 (MQ=255)                      ggcgAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAACCGTTTGTTACCGCCTGTTTAggc                               <  1:3101046/63‑1 (MQ=255)                      ggcgAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAACCGTTTGTTACCGCCTGTTTAggc                               <  1:3097022/63‑1 (MQ=255)                      ggcgAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAACCGTTTGTTACCGCCTGTTTAggc                               <  1:2960716/63‑1 (MQ=255)                      ggcgAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAACCGTTTGTTACCGCCTGTTTAggc                               <  1:2901794/63‑1 (MQ=255)                      ggcgAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAACCGTTTGTTACCGCCTGTTTAggc                               <  1:1042137/63‑1 (MQ=255)                      ggcgAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAACCGTTTGTTACCGCCTGTTTAggc                               <  1:2249832/63‑1 (MQ=255)                      ggcgAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAACCGTTTGTTACCGCCTGTTTAggc                               <  1:1268968/63‑1 (MQ=255)                            ccGCTGATTTACGGTGTCACCCTGCCCCGCATGAATCCGTTTGTTACCGCCTGTTTAggc                               <  1:1128226/60‑1 (MQ=255)                                                ccTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCt  <  1:1693960/69‑1 (MQ=255)                                                               |                                                      ATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCT  >  minE/1600844‑1600959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑