| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,602,820 | G→A | 74.9% | L262L (CTG→TTG) | yfeX ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,602,820 | 0 | G | A | 74.9% | 125.8 / 39.4 | 107 | L262L (CTG→TTG) | yfeX | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (6/21); new base A (26/54); total (32/75) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
Only 100 of 107 total aligned reads displayed.
TTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCC > minE/1602761‑1602880 | tttGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTc < 1:1357030/69‑1 (MQ=255) gAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGt < 1:966471/68‑1 (MQ=255) gAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGt < 1:226555/68‑1 (MQ=255) gAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGt < 1:230430/68‑1 (MQ=255) gAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGt < 1:2482186/68‑1 (MQ=255) gAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGt < 1:828152/68‑1 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:2032713/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:1955363/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:1910913/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:2510967/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:2569580/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:2578006/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:892354/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:1735267/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:1723972/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:2425071/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:101072/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:2801629/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:29193/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:3063209/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:3078991/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:204034/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:2062358/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:213274/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:3361729/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:385481/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:426801/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:827601/1‑58 (MQ=255) aaaCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgc > 1:1220394/1‑57 (MQ=255) aaaCGCAACATCGAATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATtgct > 1:88135/1‑58 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:2414833/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:2284459/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:902739/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:2278168/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:2493180/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:2608561/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:2736988/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:2752778/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:2824343/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:2869888/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:2930880/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:3157962/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:3176032/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:3322346/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:395100/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:682037/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:2027128/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:1055073/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:1345765/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:1457546/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:1522247/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:1661548/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:1817083/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:1824859/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:1927036/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:196576/69‑1 (MQ=255) aCGCAACATCGCATCACGCTTACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:1973704/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATg < 1:715068/49‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:1234649/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:3396381/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:113429/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:1108743/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:3194941/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:1084920/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:1421560/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:366244/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:1495421/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:2134714/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:1970583/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:1245218/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:1576382/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:2828405/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:454425/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:163158/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:2422950/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:460341/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:2735963/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:745051/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:1963749/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:1846861/69‑1 (MQ=255) tACCATCAGTATCGCCAAACATACTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:2326799/69‑1 (MQ=255) aCCATCAGTATCGCCAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:243243/68‑1 (MQ=255) aTCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAg > 1:3399945/1‑69 (MQ=255) aTCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAg > 1:359727/1‑69 (MQ=255) aTCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAg < 1:1245888/69‑1 (MQ=255) aTCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAg > 1:1535742/1‑69 (MQ=255) aTCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAg < 1:2912681/69‑1 (MQ=255) aTCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAg > 1:1596761/1‑69 (MQ=255) aTCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAg < 1:1694912/69‑1 (MQ=255) aTCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAg > 1:2638284/1‑69 (MQ=255) aTCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAg > 1:740779/1‑69 (MQ=255) ccAAACATGCTCAGCAATTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:1021222/55‑1 (MQ=255) aaaCATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTg < 1:1220994/69‑1 (MQ=255) aaaCATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTg < 1:761231/69‑1 (MQ=255) aaaCATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTg < 1:1151162/69‑1 (MQ=255) aaaCATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTg < 1:3004812/69‑1 (MQ=255) aaaCATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTg < 1:1760706/69‑1 (MQ=255) aaCATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTg < 1:1332004/68‑1 (MQ=255) gCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGcc < 1:3269805/69‑1 (MQ=255) cTCAGCAGTTGCTGTTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGcc < 1:1390462/68‑1 (MQ=255) | TTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCC > minE/1602761‑1602880 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |