| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,602,887 | A→G | 45.2% | T239T (ACT→ACC) | yfeX ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,602,887 | 0 | A | G | 45.2% | 7.0 / 50.0 | 62 | T239T (ACT→ACC) | yfeX | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (28/6); new base G (18/10); total (46/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCG > minE/1602823‑1602952 | gctgctCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGc > 1:3173321/1‑69 (MQ=255) cAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTAc < 1:2729112/68‑1 (MQ=255) cAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTAc > 1:1394353/1‑68 (MQ=255) cAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTAc < 1:1517069/68‑1 (MQ=255) aTGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCGGGct > 1:362646/1‑69 (MQ=255) aTGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGct > 1:1155532/1‑69 (MQ=255) aTGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGct > 1:1033607/1‑69 (MQ=255) aTGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGct > 1:744452/1‑69 (MQ=255) aTGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGct > 1:627063/1‑69 (MQ=255) aTGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGct > 1:197181/1‑69 (MQ=255) cgcgcgCAGTAGGCGCAGAAGTACAGTCCGTGAGTGCCACTGGCa < 1:509966/45‑1 (MQ=255) cgcgcgCAGTAGGCGCAGAAGTACAGACCGTGATTGCCACTGGCa < 1:2672782/45‑1 (MQ=255) cgcgcgCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCa < 1:1954916/45‑1 (MQ=255) cgcgcgCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCa < 1:2813501/45‑1 (MQ=255) cgcgcgCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCa < 1:3315556/45‑1 (MQ=255) cgcgcgCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCa < 1:1148765/45‑1 (MQ=255) cgcgcgCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCa < 1:1536687/45‑1 (MQ=255) cgcgcgCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCa < 1:1482213/45‑1 (MQ=255) cgcgcgCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCg > 1:604395/1‑68 (MQ=255) cgcgcgCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCg > 1:608602/1‑68 (MQ=255) cgcAGTAGGCGCAGAAGTACAGCCCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACaa > 1:2259127/1‑69 (MQ=255) cgcAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACaa > 1:1051991/1‑69 (MQ=255) cgcAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACaa > 1:2528255/1‑69 (MQ=255) cgcAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACaa > 1:2524372/1‑69 (MQ=255) cgcAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACaa > 1:2263172/1‑69 (MQ=255) gcAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAAt < 1:3186270/69‑1 (MQ=255) gcAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAAt < 1:2961535/69‑1 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGCCGAACAATCTTCAGccc > 1:569035/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:920619/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:3244002/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:3314284/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:962921/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:354087/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:362729/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:970749/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:2142427/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:1908525/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:1552398/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:2044281/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:2079806/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:213127/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:1415997/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:1215879/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:2518006/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:1182025/1‑69 (MQ=255) tACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACaa > 1:240294/1‑52 (MQ=255) tACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCa > 1:673127/1‑69 (MQ=255) tACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCa > 1:643064/1‑69 (MQ=255) tACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCa > 1:935524/1‑69 (MQ=255) tACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCa > 1:2744181/1‑69 (MQ=255) cAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCa > 1:1441776/1‑67 (MQ=255) cAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCAtc > 1:1966076/1‑69 (MQ=255) cAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCAt > 1:714254/1‑68 (MQ=255) cAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCAt > 1:443523/1‑68 (MQ=255) cAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCAt > 1:2052418/1‑68 (MQ=255) cAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCAt > 1:2201295/1‑68 (MQ=255) cAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCAt > 1:3120329/1‑68 (MQ=255) cAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCAt > 1:2863794/1‑68 (MQ=255) gCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:1734659/69‑1 (MQ=255) gCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:1633577/69‑1 (MQ=255) gCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:609600/69‑1 (MQ=255) gCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:2065976/69‑1 (MQ=255) gCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:3291963/69‑1 (MQ=255) gCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:713357/69‑1 (MQ=255) gCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:855566/69‑1 (MQ=255) gCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:2758014/69‑1 (MQ=255) gCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:119743/69‑1 (MQ=255) gCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:2696782/69‑1 (MQ=255) ccACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:760614/68‑1 (MQ=255) ggCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAAcgcg < 1:2577051/69‑1 (MQ=255) | GCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCG > minE/1602823‑1602952 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |