| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,602,908 | G→A | 39.7% | R232R (CGC→CGT) | yfeX ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,602,908 | 0 | G | A | 39.7% | 32.0 / 44.3 | 69 | R232R (CGC→CGT) | yfeX | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (22/19); new base A (17/10); total (40/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACG > minE/1602843‑1602976 | cgcgcgCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCg > 1:608602/1‑68 (MQ=255)cgcgcgCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCg > 1:604395/1‑68 (MQ=255) cgcAGTAGGCGCAGAAGTACAGCCCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACaa > 1:2259127/1‑69 (MQ=255) cgcAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACaa > 1:1051991/1‑69 (MQ=255) cgcAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACaa > 1:2263172/1‑69 (MQ=255) cgcAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACaa > 1:2528255/1‑69 (MQ=255) cgcAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACaa > 1:2524372/1‑69 (MQ=255) gcAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAAt < 1:3186270/69‑1 (MQ=255) gcAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAAt < 1:2961535/69‑1 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGCCGAACAATCTTCAGccc > 1:569035/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:2079806/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:970749/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:213127/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:2142427/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:2518006/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:3244002/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:3314284/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:354087/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:362729/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:920619/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:962921/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:1182025/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:1552398/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:1908525/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:1415997/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:1215879/1‑69 (MQ=255) gcAGAAGTACAGACCGTGAGTGCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGccc > 1:2044281/1‑69 (MQ=255) tACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACaa > 1:240294/1‑52 (MQ=255) tACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCa > 1:673127/1‑69 (MQ=255) tACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCa > 1:643064/1‑69 (MQ=255) tACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCa > 1:935524/1‑69 (MQ=255) tACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCa > 1:2744181/1‑69 (MQ=255) cAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCa > 1:1441776/1‑67 (MQ=255) cAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCAtc > 1:1966076/1‑69 (MQ=255) cAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCAt > 1:714254/1‑68 (MQ=255) cAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCAt > 1:443523/1‑68 (MQ=255) cAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCAt > 1:2052418/1‑68 (MQ=255) cAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCAt > 1:3120329/1‑68 (MQ=255) cAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCAt > 1:2863794/1‑68 (MQ=255) cAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCAt > 1:2201295/1‑68 (MQ=255) gCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:119743/69‑1 (MQ=255) gCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:855566/69‑1 (MQ=255) gCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:713357/69‑1 (MQ=255) gCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:609600/69‑1 (MQ=255) gCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:1633577/69‑1 (MQ=255) gCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:3291963/69‑1 (MQ=255) gCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:1734659/69‑1 (MQ=255) gCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:2065976/69‑1 (MQ=255) gCCACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:2758014/69‑1 (MQ=255) gCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:2696782/69‑1 (MQ=255) ccACTGGCGGTGCCGTACGGCAGGCTCTGACGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATc < 1:760614/68‑1 (MQ=255) ggCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAAcgcg < 1:2577051/69‑1 (MQ=255) gTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGggtg < 1:1769768/69‑1 (MQ=255) gTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGggtg < 1:735695/69‑1 (MQ=255) gTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGggtg < 1:538159/69‑1 (MQ=255) gTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGggtg < 1:2691570/69‑1 (MQ=255) gTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGggtg < 1:2033776/69‑1 (MQ=255) gTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGggtg < 1:1808056/69‑1 (MQ=255) gTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGggtg < 1:3286268/69‑1 (MQ=255) gCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGagag < 1:440030/68‑1 (MQ=255) gCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGagag < 1:2477314/68‑1 (MQ=255) gCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGagag < 1:2401491/68‑1 (MQ=255) gCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGagag < 1:2579221/68‑1 (MQ=255) gCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGagag < 1:3102995/68‑1 (MQ=255) gCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGagag < 1:2534282/68‑1 (MQ=255) ctctGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTc > 1:2247804/1‑68 (MQ=255) ctctGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTc > 1:219563/1‑68 (MQ=255) tGGCGAACAATCTTCATCCCTTTGCCATCTTCTTTCAGATCAACGCGggtg < 1:1947815/51‑1 (MQ=255) tGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGggtg < 1:1943768/51‑1 (MQ=255) gCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACg > 1:1478983/1‑69 (MQ=255) gCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACg > 1:1827220/1‑69 (MQ=255) gCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACg > 1:29954/1‑69 (MQ=255) gCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACg > 1:1008209/1‑69 (MQ=255) | CGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACG > minE/1602843‑1602976 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |