| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,602,989 | G→A | 18.2% | I205I (ATC→ATT) | yfeX ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,602,989 | 0 | G | A | 18.2% | 121.1 / 14.8 | 76 | I205I (ATC→ATT) | yfeX | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (41/21); new base A (5/9); total (46/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.64e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 102 total aligned reads displayed.
GCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAG > minE/1602922‑1603057 | gCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTTCGGACGTTCGTCGCCGTCg < 1:641081/68‑1 (MQ=255)gCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCTCCGTCg < 1:435551/68‑1 (MQ=255)gCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCg < 1:526053/68‑1 (MQ=255)gCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCg < 1:553688/68‑1 (MQ=255)gCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCg < 1:1662516/68‑1 (MQ=255)gCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCg < 1:2640867/68‑1 (MQ=255) ccATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCtt < 1:1379681/67‑1 (MQ=255) ccATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCtt < 1:3397009/67‑1 (MQ=255) cttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTgg > 1:1916333/1‑69 (MQ=255) cttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTgg > 1:1273091/1‑69 (MQ=255) cttcttACAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTgg > 1:437785/1‑69 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGAtctc > 1:1458893/1‑60 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGAtctc > 1:3101305/1‑60 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGAtctc > 1:78120/1‑60 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGAtctc > 1:1527477/1‑60 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGAtctc > 1:2213025/1‑60 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGAtctc > 1:566120/1‑60 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGAtctc > 1:499995/1‑60 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGAtctc > 1:1404423/1‑60 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGAtctc > 1:1401316/1‑60 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGAtctc > 1:838094/1‑60 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGAtctc > 1:2644711/1‑60 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGAtctc > 1:3297685/1‑60 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGAtctc > 1:1207446/1‑60 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGAtctc > 1:311321/1‑60 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTgg > 1:3345384/1‑68 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGc > 1:411285/1‑69 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGc > 1:2195220/1‑69 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGc > 1:282587/1‑69 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGc > 1:3213239/1‑69 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGc > 1:302043/1‑69 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGc > 1:2002377/1‑69 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGc > 1:1013988/1‑69 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGc > 1:1964524/1‑69 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGc > 1:549752/1‑69 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGc > 1:1000072/1‑69 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGc > 1:1110321/1‑69 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGc > 1:1290527/1‑69 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGc > 1:1307318/1‑69 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGc > 1:55599/1‑69 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGc > 1:1879309/1‑69 (MQ=255) ttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTAGATCTCTTCGTTGGc > 1:500514/1‑69 (MQ=255) aGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTTGCCGTCGATCTCTTCGTTGGCCTCtt < 1:1344866/67‑1 (MQ=255) aGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCtt < 1:1211778/67‑1 (MQ=255) aGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCtt < 1:3088443/67‑1 (MQ=255) aGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCtt < 1:849767/67‑1 (MQ=255) aGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCtt < 1:3253010/67‑1 (MQ=255) aGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCtt < 1:1110621/67‑1 (MQ=255) aGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCtt < 1:3263065/67‑1 (MQ=255) aGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCtt < 1:2187117/67‑1 (MQ=255) aGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCtt < 1:790629/67‑1 (MQ=255) aGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCtt < 1:254111/67‑1 (MQ=255) aGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCtt < 1:400317/67‑1 (MQ=255) aGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCtt < 1:2320513/67‑1 (MQ=255) aGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCtt < 1:610697/67‑1 (MQ=255) aGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCtt < 1:1682955/67‑1 (MQ=255) aGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCCCCGTCGATCTCTTCGTTGGCCTCtt < 1:2887556/67‑1 (MQ=255) gATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCtt < 1:278911/66‑1 (MQ=255) gATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCtt < 1:1806337/66‑1 (MQ=255) cAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTgc > 1:841703/1‑69 (MQ=255) cAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTgc > 1:928324/1‑69 (MQ=255) cAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTgc > 1:2911858/1‑69 (MQ=255) cAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTgc > 1:1926106/1‑69 (MQ=255) tcaggtgagAGGTTTCCGGACGTTCGTCGCCGTCAATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCa < 1:1107883/67‑1 (MQ=255) tcaggtgagAGGTTTCCGGACGTTCGTCGCCGTCAATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCa < 1:2871930/67‑1 (MQ=255) tcaggtgagAGGTTTCCGGACGTTCGTCGCCGTCAATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCa < 1:661944/67‑1 (MQ=255) tcaggtgagAGGTTTCCGGACGTTCGTCGCCGTCAATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCa < 1:1919680/67‑1 (MQ=255) tcaggtgagAGGTTTCCGGACGTTCGTCGCCGTCAATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCa < 1:3354158/67‑1 (MQ=255) tcaggtgagAGGTTTCCGGACGTTCGTCGCCGTCAATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCa < 1:2275372/67‑1 (MQ=255) tcaggtgagAGGTTTCCGGACGTTCGTCGCCGTCAATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCa < 1:488736/67‑1 (MQ=255) cGGACGTTCGTCGCCGTCAATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCa < 1:1915577/53‑1 (MQ=255) ttCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCCCCATCTCCTGATCGTGAACg > 1:1487028/1‑67 (MQ=255) ttCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCt > 1:3311317/1‑69 (MQ=255) ttCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCt > 1:550344/1‑69 (MQ=255) tcgtcgCCGTCAATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCATCATCTCTTGATCGTg > 1:37110/1‑62 (MQ=255) tcgtcgCCGTCAATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCATCATCTCTTGATCGTGAACGCTc > 1:1035600/1‑69 (MQ=255) tcgtcgCCGTCAATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCATCATCTCTTGATCGTGAACGCTc > 1:1953264/1‑69 (MQ=255) tcgtcgCCGTCAATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCATCATCTCTTGATCGTGAACGCTc > 1:2573454/1‑69 (MQ=255) tcgtcgCCGTCAATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCATCATCTCTTGATCGTGAACGCTc > 1:2724526/1‑69 (MQ=255) cgtcgCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCa < 1:1290486/69‑1 (MQ=255) gTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGtt < 1:2634540/69‑1 (MQ=255) gATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGc < 1:1636325/56‑1 (MQ=255) gATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGc < 1:302286/56‑1 (MQ=255) gATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGc < 1:1232342/56‑1 (MQ=255) gATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGc < 1:1235709/56‑1 (MQ=255) gATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGc < 1:289282/56‑1 (MQ=255) gATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGc < 1:778515/56‑1 (MQ=255) gATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGc < 1:736851/56‑1 (MQ=255) gATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGc < 1:1506423/56‑1 (MQ=255) gATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGc < 1:2185033/56‑1 (MQ=255) gATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGc < 1:1758137/56‑1 (MQ=255) gATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGc < 1:2676554/56‑1 (MQ=255) gATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGc < 1:3321820/56‑1 (MQ=255) gATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGc < 1:2388763/56‑1 (MQ=255) gATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGc < 1:2181120/56‑1 (MQ=255) gATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAg > 1:634221/1‑69 (MQ=255) gATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAg > 1:1931508/1‑69 (MQ=255) gATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAg > 1:203368/1‑69 (MQ=255) gATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAg > 1:415583/1‑69 (MQ=255) gATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAg > 1:3205301/1‑69 (MQ=255) | GCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAG > minE/1602922‑1603057 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |