| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,605,751 | T→G | 31.1% | L114L (CTT→CTG) | amiA → | N‑acetylmuramoyl‑l‑alanine amidase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,605,751 | 0 | T | G | 31.1% | 162.0 / 118.5 | 164 | L114L (CTT→CTG) | amiA | N‑acetylmuramoyl‑l‑alanine amidase I |
| Reads supporting (aligned to +/- strand): ref base T (52/61); new base G (15/36); total (67/97) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.89e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 | |||||||||||
Only 100 of 166 total aligned reads displayed.
CCATTTTGCGTAATCATGGGATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCC > minE/1605687‑1605811 | ccATTTTGCGTAATCATGGGATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACg > 1:1757199/1‑69 (MQ=255)ccATTTTGCGTAATCATGGGATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACg > 1:1631938/1‑69 (MQ=255)ccATTTTGCGTAATCATGGGATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACg > 1:445221/1‑69 (MQ=255)ccATTTTGCGTAATCATGGGATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACg > 1:946872/1‑69 (MQ=255) ttGCGTAATCATGGGATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATcgc > 1:558897/1‑69 (MQ=255) ttGCGTAATCATGGGATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATcgc > 1:252079/1‑69 (MQ=255) ttGCGTAATCATGGGATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATcgc > 1:108838/1‑69 (MQ=255) ttGCGTAATCATGGGATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATcgc > 1:308292/1‑69 (MQ=255) ttGCGTAATCATGGGATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATcgc > 1:1807487/1‑69 (MQ=255) ttGCGTAATCATGGGATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATcgc > 1:1755057/1‑69 (MQ=255) ttGCGTAATCATGGGATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATcgc > 1:3305668/1‑69 (MQ=255) ttGCGTAATCATGGGATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACAATcgc > 1:1019781/1‑69 (MQ=255) cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:1577198/69‑1 (MQ=255) cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:1849381/69‑1 (MQ=255) cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:742394/69‑1 (MQ=255) cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:1796158/69‑1 (MQ=255) cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:3068621/69‑1 (MQ=255) cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:2951836/69‑1 (MQ=255) cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:3425678/69‑1 (MQ=255) cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:286459/69‑1 (MQ=255) cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:283151/69‑1 (MQ=255) cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:2742745/69‑1 (MQ=255) cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:3265350/69‑1 (MQ=255) cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:2292436/69‑1 (MQ=255) cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:234094/69‑1 (MQ=255) cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:1290072/69‑1 (MQ=255) cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:254728/69‑1 (MQ=255) cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:1292358/69‑1 (MQ=255) cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:1296455/69‑1 (MQ=255) cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:130293/69‑1 (MQ=255) aTTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATCGCCCa < 1:512797/68‑1 (MQ=255) aTTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATCGCCCa < 1:2191593/68‑1 (MQ=255) aTTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATCGCCCa < 1:3123911/68‑1 (MQ=255) aTTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATCGCCCa < 1:1010726/68‑1 (MQ=255) aTTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATCGCCCa < 1:1785754/68‑1 (MQ=255) aTTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATCGCCCa < 1:1167114/68‑1 (MQ=255) gATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:1146063/69‑1 (MQ=255) gATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:2139864/69‑1 (MQ=255) gATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:2629389/69‑1 (MQ=255) gATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATCGCCCa < 1:2644099/65‑1 (MQ=255) aTGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:1397093/68‑1 (MQ=255) aTGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:274200/68‑1 (MQ=255) tGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:870916/67‑1 (MQ=255) tGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:148693/67‑1 (MQ=255) tGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:1087127/67‑1 (MQ=255) tGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:1273074/67‑1 (MQ=255) tGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:501148/67‑1 (MQ=255) gTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGgcg < 1:1597699/69‑1 (MQ=255) tAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATgg > 1:1090221/1‑64 (MQ=255) tAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATgg > 1:2436349/1‑64 (MQ=255) tAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATgg > 1:2351415/1‑64 (MQ=255) tAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATgg > 1:2303292/1‑64 (MQ=255) tAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAg > 1:3150670/1‑69 (MQ=255) aaCGCGTTCTGGCGATACGTTTATCCCACTTTACg > 1:409044/1‑35 (MQ=255) aaCGCGTTCTGGCGATACGTTTATCCCACTTTACg > 1:3391264/1‑35 (MQ=255) aaCGCGTTCTGGCGATACGTTTATCCCACTTTACg > 1:1316367/1‑35 (MQ=255) aaCGCGTTCTGGCGATACGTTTATCCCACTTTACg > 1:938864/1‑35 (MQ=255) aaCGCGTTCTGGCGATACGTTTATCCCACTTTACg > 1:2268794/1‑35 (MQ=255) aaCGCGTTCTGGCGATACGTTTATCCCACTTTACg > 1:2226477/1‑35 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:2482056/1‑69 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1247453/1‑69 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:3334399/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:1496762/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:3224015/1‑69 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:3214388/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:3210424/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:1542277/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1932207/1‑69 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:2023591/1‑69 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:2083035/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:2274470/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:628110/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:568769/69‑1 (MQ=255) tCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:2754789/68‑1 (MQ=255) tCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:901190/68‑1 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:701412/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:462733/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:498261/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:67483/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:977726/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:1408212/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:2796265/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:2566684/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:1587072/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:2353333/1‑68 (MQ=255) taacccACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATg > 1:2555619/4‑68 (MQ=255) tATCCTACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCaa < 1:2499350/62‑1 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1111890/1‑54 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1319736/1‑54 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:2658287/1‑54 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGt > 1:2919258/1‑53 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCaa < 1:2447629/62‑1 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATg > 1:2070538/1‑68 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATg > 1:482195/1‑68 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATg > 1:475762/1‑68 (MQ=255) tATCCCACTTTACGATCGCCTTGAAATCGCCCATAAACATGGCGCAGATc > 1:1427936/1‑50 (MQ=255) tCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGcc < 1:1811357/68‑1 (MQ=255) tCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGcc < 1:338006/68‑1 (MQ=255) tCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGcc < 1:453587/68‑1 (MQ=255) ttACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:927004/45‑1 (MQ=255) | CCATTTTGCGTAATCATGGGATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCC > minE/1605687‑1605811 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |