| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,605,763 | T→G | 34.7% | V118V (GTT→GTG) | amiA → | N‑acetylmuramoyl‑l‑alanine amidase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,605,763 | 0 | T | G | 34.7% | 109.2 / 121.6 | 147 | V118V (GTT→GTG) | amiA | N‑acetylmuramoyl‑l‑alanine amidase I |
| Reads supporting (aligned to +/- strand): ref base T (31/65); new base G (15/36); total (46/101) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 147 total aligned reads displayed.
CATGGGATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTA > minE/1605701‑1605821 | cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:1296455/69‑1 (MQ=255)cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:1577198/69‑1 (MQ=255)cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:2353143/69‑1 (MQ=255)cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:254728/69‑1 (MQ=255)cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:2556141/69‑1 (MQ=255)cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:2338581/69‑1 (MQ=255)cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:742394/69‑1 (MQ=255)cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:1407930/69‑1 (MQ=255)cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:3425678/69‑1 (MQ=255)cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:2742745/69‑1 (MQ=255)cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:130293/69‑1 (MQ=255)cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:286459/69‑1 (MQ=255)cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:1290072/69‑1 (MQ=255)cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:3265350/69‑1 (MQ=255)cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:2951836/69‑1 (MQ=255)cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:2292436/69‑1 (MQ=255)cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:1158317/69‑1 (MQ=255)cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:3068621/69‑1 (MQ=255)cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:1796158/69‑1 (MQ=255)cATGGCATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATc < 1:505425/69‑1 (MQ=255) aTTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATCGCCCa < 1:512797/68‑1 (MQ=255) aTTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATCGCCCa < 1:2191593/68‑1 (MQ=255) aTTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATCGCCCa < 1:1167114/68‑1 (MQ=255) aTTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATCGCCCa < 1:3123911/68‑1 (MQ=255) aTTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATCGCCCa < 1:1010726/68‑1 (MQ=255) tGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATCGCCCa < 1:3185810/66‑1 (MQ=255) gATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:2526348/69‑1 (MQ=255) gATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:2629389/69‑1 (MQ=255) gATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:2268792/69‑1 (MQ=255) gATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:2139864/69‑1 (MQ=255) gATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:902707/69‑1 (MQ=255) gATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:614278/69‑1 (MQ=255) gATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTGTACGATCGCGTGGAAATCGCCCa < 1:2644099/65‑1 (MQ=255) tGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:3218251/67‑1 (MQ=255) tGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:870916/67‑1 (MQ=255) tGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:1273074/67‑1 (MQ=255) tGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:1769632/67‑1 (MQ=255) tGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:3073556/67‑1 (MQ=255) tGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:1087127/67‑1 (MQ=255) tGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATaaa < 1:2134177/67‑1 (MQ=255) gTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGgcg < 1:1597699/69‑1 (MQ=255) tAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATgg > 1:1090221/1‑64 (MQ=255) tAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATgg > 1:451891/1‑64 (MQ=255) tAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATgg > 1:2765635/1‑64 (MQ=255) tAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATgg > 1:2436349/1‑64 (MQ=255) tAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATgg > 1:2351415/1‑64 (MQ=255) tAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATgg > 1:2303292/1‑64 (MQ=255) tAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAg > 1:3150670/1‑69 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:3334399/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:3353829/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1247453/1‑69 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:3421363/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:3214388/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:958944/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1330926/1‑69 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:1496762/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:2356228/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:568769/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:2023591/1‑69 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:48867/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:1787308/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:2274470/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:1748517/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:705450/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:245217/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:2482056/1‑69 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:65162/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1932207/1‑69 (MQ=255) tCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCATATCTGtt < 1:2659415/68‑1 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:462733/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:977726/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:498261/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:67483/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:1932714/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:1047646/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:1408212/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:1488004/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:1587072/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:2566684/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:2841310/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:2796265/1‑68 (MQ=255) tATCCTACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCaa < 1:2499350/62‑1 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1111890/1‑54 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1319736/1‑54 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1543411/1‑54 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:2658287/1‑54 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGt > 1:2919258/1‑53 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCaa < 1:1831966/62‑1 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATg > 1:2743256/1‑68 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATg > 1:1290256/1‑68 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATg > 1:2070538/1‑68 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATg > 1:482195/1‑68 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATg > 1:475762/1‑68 (MQ=255) tATCCCACTTTACGATCGCCTTGAAATCGCCCATAAACATGGCGCAGATc > 1:1427936/1‑50 (MQ=255) tCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGcc < 1:1811357/68‑1 (MQ=255) tCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGcc < 1:326306/68‑1 (MQ=255) tCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGcc < 1:2054120/68‑1 (MQ=255) tCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGcc < 1:453587/68‑1 (MQ=255) ttACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:927004/45‑1 (MQ=255) cGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTa < 1:861832/68‑1 (MQ=255) | CATGGGATTGATGCGCGTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTA > minE/1605701‑1605821 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |