| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,605,781 | T→C | 18.3% | H124H (CAT→CAC) | amiA → | N‑acetylmuramoyl‑l‑alanine amidase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,605,781 | 0 | T | C | 18.3% | 182.7 / 28.0 | 119 | H124H (CAT→CAC) | amiA | N‑acetylmuramoyl‑l‑alanine amidase I |
| Reads supporting (aligned to +/- strand): ref base T (54/43); new base C (15/7); total (69/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
Only 100 of 124 total aligned reads displayed.
GTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGT > minE/1605717‑1605849 | gTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGgcg < 1:1597699/69‑1 (MQ=255) tAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATgg > 1:366049/1‑64 (MQ=255) tAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATgg > 1:1090221/1‑64 (MQ=255) tAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATgg > 1:2765635/1‑64 (MQ=255) tAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATgg > 1:2436349/1‑64 (MQ=255) tAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATgg > 1:2184880/1‑64 (MQ=255) tAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATgg > 1:2351415/1‑64 (MQ=255) tAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATgg > 1:1278411/1‑64 (MQ=255) tAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAg > 1:3150670/1‑69 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:2294409/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:2900045/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:2274470/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1089582/1‑69 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:2356228/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:2083035/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:3210424/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:2023591/1‑69 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:245217/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:3214388/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:3224015/1‑69 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:3334399/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:3421363/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1932207/1‑69 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:2482056/1‑69 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:476515/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:1748517/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:48867/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:958944/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:568769/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:1542277/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:628110/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:1496762/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:65162/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:750743/69‑1 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1247453/1‑69 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1330926/1‑69 (MQ=255) ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:705450/69‑1 (MQ=255) tCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCATATCTGtt < 1:2659415/68‑1 (MQ=255) tCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:901190/68‑1 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:701412/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:2841310/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:3138047/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:2796265/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:498261/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:1047646/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:977726/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:462733/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:2566684/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:1408212/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:2353333/1‑68 (MQ=255) taacccACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATg > 1:2555619/4‑68 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1319736/1‑54 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1543411/1‑54 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1614128/1‑54 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:460806/1‑54 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1111890/1‑54 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:2658287/1‑54 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGt > 1:2919258/1‑53 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCaa < 1:2447629/62‑1 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCaa < 1:1831966/62‑1 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATg > 1:2743256/1‑68 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATg > 1:2070538/1‑68 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATg > 1:482195/1‑68 (MQ=255) tATCCCACTTTACGATCGCCTTGAAATCGCCCATAAACATGGCGCAGATc > 1:1427936/1‑50 (MQ=255) tCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGcc < 1:1811357/68‑1 (MQ=255) tCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGcc < 1:326306/68‑1 (MQ=255) tCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGcc < 1:453587/68‑1 (MQ=255) tCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGcc < 1:338006/68‑1 (MQ=255) ttACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:927004/45‑1 (MQ=255) ttACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:1210332/45‑1 (MQ=255) aTCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTa < 1:200862/66‑1 (MQ=255) gAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGaaa > 1:318909/1‑69 (MQ=255) gAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGaaa > 1:1997699/1‑69 (MQ=255) aaaTCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCACGCCGACGGTTTTACCAAc < 1:2681066/62‑1 (MQ=255) aaaTCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCACGCCGACGGTTTTACCAAc < 1:1242439/62‑1 (MQ=255) aaaTCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCACGCCGACGGTTTTACCAAc < 1:2760816/62‑1 (MQ=255) aaaTCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCACGCCGACGGTTTTACCAAc < 1:429010/62‑1 (MQ=255) aaaTCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCACGCCGACGGTTTTACCAAc < 1:3276798/62‑1 (MQ=255) aaTCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCACGCCGACGGTTTTACCAAc < 1:1737809/61‑1 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:1911441/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:78794/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:263846/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:46101/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:3077431/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:364407/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:3008078/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:2964791/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:2283325/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:1514579/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:2746564/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCt > 1:2244456/1‑68 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATACCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:3068604/1‑69 (MQ=255) cATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTTCCAACCCGAAAGCTGCCGGTGCTTc > 1:2227352/1‑68 (MQ=255) cATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTc > 1:1101430/1‑68 (MQ=255) cATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTc > 1:2019128/1‑68 (MQ=255) cATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTc > 1:2637484/1‑68 (MQ=255) cATGGCGCAGATCTGTTTATGTCAATTCATCCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTc > 1:539958/1‑68 (MQ=255) tGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGt < 1:2007635/69‑1 (MQ=255) tGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGt < 1:3178286/69‑1 (MQ=255) tGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGt < 1:2365550/69‑1 (MQ=255) | GTTTAACGCGTTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGT > minE/1605717‑1605849 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |