| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,605,790 | T→C | 18.2% | D127D (GAT→GAC) | amiA → | N‑acetylmuramoyl‑l‑alanine amidase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,605,790 | 0 | T | C | 18.2% | 185.5 / 27.8 | 120 | D127D (GAT→GAC) | amiA | N‑acetylmuramoyl‑l‑alanine amidase I |
| Reads supporting (aligned to +/- strand): ref base T (45/53); new base C (15/7); total (60/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.72e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
Only 100 of 121 total aligned reads displayed.
TTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCT > minE/1605727‑1605858 | ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:3033629/69‑1 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:3353829/69‑1 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:3334399/69‑1 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:1748517/69‑1 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:1787308/69‑1 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:3224015/1‑69 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:3214388/69‑1 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:3210424/69‑1 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1932207/1‑69 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:958944/69‑1 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:2900045/69‑1 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:2023591/1‑69 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:2654930/69‑1 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:2083035/69‑1 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:2482056/1‑69 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:245217/69‑1 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:2356228/69‑1 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:2274470/69‑1 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:1542277/69‑1 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:3421363/69‑1 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:476515/69‑1 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:534992/69‑1 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1330926/1‑69 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:568769/69‑1 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:628110/69‑1 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1247453/1‑69 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1089582/1‑69 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:750743/69‑1 (MQ=255)ttCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:705450/69‑1 (MQ=255) tCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCATATCTGtt < 1:2659415/68‑1 (MQ=255) tCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:901190/68‑1 (MQ=255) tCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:2754789/68‑1 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:2353333/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:977726/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:701412/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:67483/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:2796265/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:498261/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:462733/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:1488004/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:1587072/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:1932714/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:1408212/1‑68 (MQ=255) tttATCCCACTGTACGATCGCGTGGAAATCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCac > 1:1047646/1‑68 (MQ=255) taacccACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATg > 1:2555619/4‑68 (MQ=255) tATCCTACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCaa < 1:2499350/62‑1 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1614128/1‑54 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1319736/1‑54 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:1543411/1‑54 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt > 1:2658287/1‑54 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGt > 1:2919258/1‑53 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCaa < 1:2447629/62‑1 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCaa < 1:1831966/62‑1 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATg > 1:2743256/1‑68 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATg > 1:482195/1‑68 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATg > 1:2070538/1‑68 (MQ=255) tATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATg > 1:475762/1‑68 (MQ=255) tATCCCACTTTACGATCGCCTTGAAATCGCCCATAAACATGGCGCAGATc > 1:1427936/1‑50 (MQ=255) tCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGcc < 1:1811357/68‑1 (MQ=255) tCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGcc < 1:2054120/68‑1 (MQ=255) tCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGcc < 1:453587/68‑1 (MQ=255) tCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGcc < 1:326306/68‑1 (MQ=255) ttACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:927004/45‑1 (MQ=255) ttACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGtt < 1:1210332/45‑1 (MQ=255) gAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGaaa > 1:1997699/1‑69 (MQ=255) gAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGaaa > 1:3176543/1‑69 (MQ=255) gAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGaaa > 1:318909/1‑69 (MQ=255) aaaTCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCACGCCGACGGTTTTACCAAc < 1:429010/62‑1 (MQ=255) aaaTCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCACGCCGACGGTTTTACCAAc < 1:2760816/62‑1 (MQ=255) aaaTCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCACGCCGACGGTTTTACCAAc < 1:1242439/62‑1 (MQ=255) aaaTCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCACGCCGACGGTTTTACCAAc < 1:3276798/62‑1 (MQ=255) aaaTCGCCCATAAACACGGCGCAGACCTGTTTATGTCAATTCACGCCGACGGTTTTACCAAc < 1:223479/62‑1 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:46101/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:1514579/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:364407/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:2283325/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:78794/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:1911441/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:3077431/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:3008078/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:284447/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:2746564/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:263846/1‑69 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCt > 1:2244456/1‑68 (MQ=255) aaCATGGCGCAGATCTGTTTATGTCAATTCATACCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCtt > 1:3068604/1‑69 (MQ=255) cATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTc > 1:1101430/1‑68 (MQ=255) cATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTc > 1:2019128/1‑68 (MQ=255) cATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTc > 1:2637484/1‑68 (MQ=255) cATGGCGCAGATCTGTTTATGTCAATTCATCCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTc > 1:539958/1‑68 (MQ=255) tGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGt < 1:3178286/69‑1 (MQ=255) tGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGt < 1:2365550/69‑1 (MQ=255) tGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGt < 1:1198488/69‑1 (MQ=255) tGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGt < 1:2007635/69‑1 (MQ=255) cAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTg > 1:128318/1‑69 (MQ=255) gATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGcc < 1:2933912/69‑1 (MQ=255) gATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGcc < 1:2968248/69‑1 (MQ=255) gATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGcc < 1:2252118/69‑1 (MQ=255) gATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGcc < 1:2314080/69‑1 (MQ=255) gATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGcc < 1:1037867/69‑1 (MQ=255) tCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCct > 1:1040309/1‑69 (MQ=255) | TTCTGGCGATACGTTTATCCCACTTTACGATCGCGTTGAAATCGCCCATAAACATGGCGCAGATCTGTTTATGTCAATTCATGCCGATGGCTTTACCAACCCGAAAGCTGCCGGTGCTTCGGTATTTGCCCT > minE/1605727‑1605858 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |