| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,606,349 | C→T | 30.6% | L23L (CTG→TTG) | hemF → | coproporphyrinogen III oxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,606,349 | 0 | C | T | 30.6% | 48.7 / 22.1 | 52 | L23L (CTG→TTG) | hemF | coproporphyrinogen III oxidase |
| Reads supporting (aligned to +/- strand): ref base C (10/26); new base T (9/7); total (19/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.54e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.12e-01 | |||||||||||
TATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGG > minE/1606282‑1606407 | tATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCt < 1:2167927/69‑1 (MQ=255)tATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCt < 1:535753/69‑1 (MQ=255)tATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCt < 1:420518/69‑1 (MQ=255)tATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCt < 1:3012266/69‑1 (MQ=255)tATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCt < 1:3001603/69‑1 (MQ=255)tATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCt < 1:2264820/69‑1 (MQ=255)tATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCt < 1:2831933/69‑1 (MQ=255) aTGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCt < 1:3427550/68‑1 (MQ=255) gAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGTTGag > 1:2874288/1‑68 (MQ=255) gAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGTTGag > 1:214746/1‑68 (MQ=255) gAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGTTGag > 1:2673159/1‑68 (MQ=255) gAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGTTGag > 1:2788716/1‑68 (MQ=255) gAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGTTGag > 1:2937224/1‑68 (MQ=255) gAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGTTGag > 1:529815/1‑68 (MQ=255) gAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGTTGag > 1:68930/1‑68 (MQ=255) gAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGTTGag > 1:1101017/1‑68 (MQ=255) gAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGTTGa > 1:218726/1‑68 (MQ=255) aaaCCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGAcc > 1:1463612/1‑69 (MQ=255) aaaCCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGAcc > 1:1479163/1‑69 (MQ=255) aaCCCGACGCACACCAGGTTAAACAGTTTCTGCTAAACCTTCAGGATACGATTTGTCAGCAGCt < 1:1497856/64‑1 (MQ=255) ccGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCt < 1:2540374/61‑1 (MQ=255) gACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTc < 1:1990320/69‑1 (MQ=255) gACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTc < 1:3077878/69‑1 (MQ=255) gACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTc < 1:473145/69‑1 (MQ=255) gACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTc < 1:3339985/69‑1 (MQ=255) gACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTc < 1:2613726/69‑1 (MQ=255) gACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTc < 1:2446807/69‑1 (MQ=255) gACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTc < 1:1858657/69‑1 (MQ=255) gCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCt < 1:149440/56‑1 (MQ=255) gCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTg < 1:1437391/57‑1 (MQ=255) gCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTg < 1:3180417/57‑1 (MQ=255) cAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGAc > 1:1421117/1‑53 (MQ=255) cAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGgcgc > 1:2942860/1‑68 (MQ=255) cAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGgcgc > 1:346912/1‑68 (MQ=255) tAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACAGCCGTCGATGGCGCAGAAtt > 1:2771584/1‑69 (MQ=255) cAACCTTCAGGATACGATTTGTCAGCAGTTGAGCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGCTg < 1:3397267/69‑1 (MQ=255) cAACCTTCAGGATACGATTTGTCAGCAGTTGAGCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGCTg < 1:423265/69‑1 (MQ=255) cAACCTTCAGGATACGATTTGTCAGCAGTTGAGCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGCTg < 1:905357/69‑1 (MQ=255) cAACCTTCAGGATACGATTTGTCAGCAGTTGAGCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGCTg < 1:2241739/69‑1 (MQ=255) cAACCTTCAGGATACGATTTGTCAGCAGTTGAGCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGCTg < 1:1466675/69‑1 (MQ=255) cAACCTTCAGGATACGATTTGTCAGCAGTTGAGCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGCTg < 1:1446572/69‑1 (MQ=255) cAACCTTCAGGATACGATTTGTCAGCAGTTGAGCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGCTg < 1:1179276/69‑1 (MQ=255) cAACCTTCAGGATACGATTTGTCAGCAGCTGACCTCCGTCGATGGCGCAGAATTTGTCGAAGATAGtt < 1:1231973/68‑1 (MQ=255) cAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGtt < 1:2747760/68‑1 (MQ=255) cAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGtt < 1:2497679/68‑1 (MQ=255) cAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGtt < 1:3410515/68‑1 (MQ=255) cAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGtt < 1:1198475/68‑1 (MQ=255) cAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGta < 1:1176521/68‑2 (MQ=255) ttGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTggcgg > 1:3384354/1‑69 (MQ=255) ttGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTggcgg > 1:2143619/1‑69 (MQ=255) ttGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTggcgg > 1:1897824/1‑69 (MQ=255) ttGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTggcg > 1:3388326/1‑68 (MQ=255) | TATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGG > minE/1606282‑1606407 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |