| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,606,942 | G→A | 30.6% | R220R (CGG→CGA) | hemF → | coproporphyrinogen III oxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,606,942 | 0 | G | A | 30.6% | 86.7 / 42.3 | 91 | R220R (CGG→CGA) | hemF | coproporphyrinogen III oxidase |
| Reads supporting (aligned to +/- strand): ref base G (31/32); new base A (17/11); total (48/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
TTGCCTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTTATATCGTCGCGGTCGTTATG > minE/1606878‑1607003 | ttGCCTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAg < 1:2283270/69‑1 (MQ=255)ttGCCTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAg < 1:66009/69‑1 (MQ=255) gCCTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGc < 1:351703/68‑1 (MQ=255) gCCTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGc < 1:543176/68‑1 (MQ=255) gCCTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGc < 1:957913/68‑1 (MQ=255) gCCTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGc < 1:633288/68‑1 (MQ=255) gCCTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGc < 1:81126/68‑1 (MQ=255) gCCTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGc < 1:1994661/68‑1 (MQ=255) gCCTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGc < 1:757944/68‑1 (MQ=255) ccTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTAGAGCGACGAAAAGCg < 1:1030197/68‑1 (MQ=255) ccTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTAGAGCGACGAAAAGCg < 1:290982/68‑1 (MQ=255) ccTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTAGAGCGACGAAAAGCg < 1:3222704/68‑1 (MQ=255) ccTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTAGAGCGACGAAAAGCg < 1:2820939/68‑1 (MQ=255) ccTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTAGAGCGACGAAAAGCg < 1:2816897/68‑1 (MQ=255) ccTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTAGAGCGACGAAAAGCg < 1:608437/68‑1 (MQ=255) ccTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTAGAGCGACGAAAAGCg < 1:707578/68‑1 (MQ=255) ccTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTAGAGCGACGAAAAGCg < 1:1858331/68‑1 (MQ=255) ccTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTAGAGCGACGAAAAGCg < 1:1284505/68‑1 (MQ=255) ccTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTAGAGCGACGAAAAGCg < 1:890757/68‑1 (MQ=255) ccTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTAGAGCGAAGAAAAGCg < 1:3341400/68‑1 (MQ=255) tGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGcc < 1:3104179/68‑1 (MQ=255) tGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGcc < 1:3377101/68‑1 (MQ=255) tGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGcc < 1:2707726/68‑1 (MQ=255) tGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGcc < 1:135271/68‑1 (MQ=255) tGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGcc < 1:3170365/68‑1 (MQ=255) tGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGcc < 1:1691117/68‑1 (MQ=255) ggCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACg > 1:2246983/1‑68 (MQ=255) ggCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACg > 1:2173394/1‑68 (MQ=255) ggCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACg > 1:1512528/1‑68 (MQ=255) ggCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACg > 1:1615759/1‑68 (MQ=255) ggCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACg > 1:2772788/1‑68 (MQ=255) ggCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACg > 1:2439202/1‑68 (MQ=255) ggCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACg > 1:384107/1‑68 (MQ=255) ggCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACg > 1:2766688/1‑68 (MQ=255) ggCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACg > 1:2938385/1‑68 (MQ=255) aGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGcc < 1:2104927/58‑1 (MQ=255) aGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACGgcgagc > 1:3399082/1‑68 (MQ=255) ggCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGAt > 1:731846/1‑47 (MQ=255) ggCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACGGCgagcgcgagcgc > 1:3314931/1‑69 (MQ=255) ggCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACGGCgagcgcgagcgc > 1:2048872/1‑69 (MQ=255) ggCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACGGCgagcgcgagcgc > 1:2898975/1‑69 (MQ=255) ggCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACGGCgagcgcgagcgc > 1:2835993/1‑69 (MQ=255) ggCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACGGCgagcgcgagcgc > 1:3392070/1‑69 (MQ=255) ggCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACGGCgagcgcgagcgc > 1:1304658/1‑69 (MQ=255) ggCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACGGCgagcgcgagcgc > 1:2816007/1‑69 (MQ=255) ggCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACGGCgagcgcgagcgc > 1:2471499/1‑69 (MQ=255) ggCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACGGCgagcgcgagcgc > 1:2550784/1‑69 (MQ=255) ggCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACGGCgagcgcgagcgc > 1:2575324/1‑69 (MQ=255) gCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGc < 1:2763496/43‑1 (MQ=255) ccGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTcc < 1:928440/69‑1 (MQ=255) ccGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTcc < 1:1157445/69‑1 (MQ=255) tATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTTatat > 1:1668813/1‑69 (MQ=255) tACCAATTGTCGAGCGACGGAAAGGGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTtat > 1:138293/1‑63 (MQ=255) tACCAATTGTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTtat > 1:1076341/1‑63 (MQ=255) tACCAATTGTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTtat > 1:1980072/1‑63 (MQ=255) tACCAATTGTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTtat > 1:2452398/1‑63 (MQ=255) tACCAATTGTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTtat > 1:2638562/1‑63 (MQ=255) cAATTGTAGAGCGATGAAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTTCAGCTCTACCGTcgcg > 1:2667783/1‑69 (MQ=255) cAATTGTAGAGCGACGAAAAGCGATGGCCTACGGCGAGCGCTAGCGCAATTTTCAGCTCTACCGTcgct > 1:489594/1‑68 (MQ=255) cAATTGTAGAGCGACGAAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTTCAGCTCTAccgtcg > 1:2841887/1‑67 (MQ=255) cAATTGTAGAGCGACGAAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTTCAGCTCTAccgtcg > 1:883392/1‑67 (MQ=255) cAATTGTAGAGCGACGAAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTTCAGCTCTAccgtcg > 1:235110/1‑67 (MQ=255) cAATTGTAGAGCGACGAAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTTCAGCTCTAccgtcg > 1:2476730/1‑67 (MQ=255) cAATTGTAGAGCGACGAAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTTCAGCTCTACCGTcgcg > 1:321740/1‑69 (MQ=255) cAATTGTAGAGCGACGAAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTTCAGCTCTACCGTcgcg > 1:149185/1‑69 (MQ=255) cAATTGTAGAGCGACGAAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTTCAGCTCTACCGTcgc > 1:1513662/1‑68 (MQ=255) cAATTGTAGAGCGACGAAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTTCAGCTCTACCGTcgc > 1:917050/1‑68 (MQ=255) cAATTGTAGAGCGACGAAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTTCAGCTCTACCGTcgc > 1:654318/1‑68 (MQ=255) cAATTGTAGAGCGACGAAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTTCAGCTCTACCGTcgc > 1:2163848/1‑68 (MQ=255) cAATTGTAGAGCGACGAAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTTCAGCTCTACCGTcgc > 1:3342627/1‑68 (MQ=255) cAATTGTAGAGCGACGAAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTTCAGCTCTACCGTcgc > 1:940783/1‑68 (MQ=255) cAATTGTAGAGCGACGAAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTTCAGCTCTACCGTcgc > 1:1929518/1‑68 (MQ=255) cAATTGTAGAGCGACGAAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTTCAGCTCTACAGTcgc > 1:1403978/1‑68 (MQ=255) cAATTGTAGAGCGACGAAAAGCGATGCCCTACGGCGAGCGCGAGCGCAATTTTCAGCTCTAccgtcg > 1:862146/1‑67 (MQ=255) tGTCGAGCGACGGAAAGCGATGGGCTACGGCGAGCGAGAGCGCAATTTCCAGTta < 1:3344267/55‑1 (MQ=255) tGTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTta > 1:447697/1‑55 (MQ=255) tGTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTta < 1:2616822/55‑1 (MQ=255) tGTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTta < 1:337501/55‑1 (MQ=255) tGTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTta < 1:2818768/55‑1 (MQ=255) gTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTTATATCGTCGCGGTCGt < 1:2375599/69‑1 (MQ=255) gTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTTATATCGTCGCGGTCGt > 1:833656/1‑69 (MQ=255) gTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTTATATCGTCGCGGTCGt > 1:1596271/1‑69 (MQ=255) gTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTTATATCGTCGCGGTCGt < 1:803262/69‑1 (MQ=255) gTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTTATATCGTCGCGGTCGt > 1:1896534/1‑69 (MQ=255) gTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTTATATCGTCGCGGTCGt < 1:2279279/69‑1 (MQ=255) gTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTTATATCGTCGCGGTCGt < 1:558027/69‑1 (MQ=255) gTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTTATATCGTCGCGGTCGt < 1:3132332/69‑1 (MQ=255) gTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTTATATCGTCGCGGTCGt < 1:398538/69‑1 (MQ=255) gTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTTATATCGTCGCGGTCGt < 1:358077/69‑1 (MQ=255) gTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTTATATCGTCGCGGTCGt < 1:2739858/69‑1 (MQ=255) gCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTTATATCGTCGCGGTCGTTATg < 1:3012475/68‑1 (MQ=255) | TTGCCTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATTTACCAATTGTCGAGCGACGGAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTCCAGTTATATCGTCGCGGTCGTTATG > minE/1606878‑1607003 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |