| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,610,862 | A→C | 63.0% | S292S (TCT→TCG) | eutB ← | ethanolamine ammonia‑lyase, large subunit, heavy chain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,610,862 | 0 | A | C | 63.0% | 19.8 / 16.6 | 27 | S292S (TCT→TCG) | eutB | ethanolamine ammonia‑lyase, large subunit, heavy chain |
| Reads supporting (aligned to +/- strand): ref base A (8/2); new base C (7/10); total (15/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.98e-01 | |||||||||||
CCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCGCCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCAC > minE/1610800‑1610927 | ccatAGTTACGCGCTTCCATCGTCACCTGGTCTGCGCCGAAGTTAGCGCCAGCGGATAGCGCCGAGCCt < 1:850400/66‑1 (MQ=255)ccatAGTTACGCGCTTCCATCGTCACCTGGTCTGCGCCGAAGTTAGCGCCAGCGGATAGCGCCGAGCCt < 1:476155/66‑1 (MQ=255)ccatAGTTACGCGCTTCCATCGTCACCTGGTCTGCGCCGAAGTTAGCGCCAGCGGATAGCGCCGAGCCt < 1:45491/66‑1 (MQ=255)ccatAGTTACGCGCTTCCATCGTCACCTGGTCTGCGCCGAAGTTAGCGCCAGCGGATAGCGCCGAGCCt < 1:161320/66‑1 (MQ=255)ccatAGTTACGCGCTTCCATCGTCACCTGGTCTGCGCCGAAGTTAGCGCCAGCGGATAGCGCCGAGCCt < 1:1862668/66‑1 (MQ=255)ccatAGTTACGCGCTTCCATCGTCACCTGGTCTGCGCCGAAGTTAGCGCCAGCGGATAGCGCCGAGCCt < 1:377378/66‑1 (MQ=255)ccatAGTTACGCGCTTCCATCGTCACCTGGTCTGCGCCGAAGTTAGCGCCAGCGGATAGCGCCGAGCCt < 1:3332596/66‑1 (MQ=255)ccatAGTTACGCGCTTCCATCGTCACCTGGTCTGCGCCGAAGTTAGCGCCAGCGGATAGCGCCGAGCCt < 1:273543/66‑1 (MQ=255) catAGTTACGCGCTTCCATCGTCACCTGGTCTGCCCCGAAGTTAGCCCCAGCGGATAGCGCCGAGCCt < 1:2992850/66‑1 (MQ=255) catAGTTACGCGCTTCCATCGTCAACTGGTCTGCGCCGAAGTTAGCGCCAGCGGATAGCGCCGAGCCt < 1:1449643/66‑1 (MQ=255) cTTCCATCGTTACCTGGTCTGCGCCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGa > 1:1704129/1‑69 (MQ=255) cTTCCATCGTTACCTGGTCTGCGCCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGa > 1:179161/1‑69 (MQ=255) aGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGt > 1:1353132/1‑68 (MQ=255) aGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGt > 1:3273839/1‑68 (MQ=255) aGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGt > 1:2779911/1‑68 (MQ=255) aGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGt > 1:2245818/1‑68 (MQ=255) aGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGt > 1:2203472/1‑68 (MQ=255) aGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGt > 1:1355978/1‑68 (MQ=255) tAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCGCGcc < 1:2002504/69‑1 (MQ=255) tAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCGCGcc < 1:1998471/69‑1 (MQ=255) cgcCGAGCCTTGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCAc > 1:2768371/1‑69 (MQ=255) cgcCGAGCCTTGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCAc > 1:2537355/1‑69 (MQ=255) cgcCGAGCCTTGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCAc > 1:3192928/1‑69 (MQ=255) cgcCGAGCCTTGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCAc > 1:1569268/1‑69 (MQ=255) cgcCGAGCCTTGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCAc > 1:82689/1‑69 (MQ=255) cgcCGAGCCTTGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCAc > 1:1269724/1‑69 (MQ=255) cgcCGAGCCTTGTCCGGTTTCGAAGTAGAGACAGGTTTCCCCGGCGATACGGTTGAACTCCGCGCCCAc > 1:3220171/1‑69 (MQ=255) | CCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCGCCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCAC > minE/1610800‑1610927 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |