| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 125,477 | T→A | 24.9% | V163V (GTA→GTT) | gcd ← | glucose dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 125,477 | 0 | T | A | 24.9% | 44.9 / 11.8 | 32 | V163V (GTA→GTT) | gcd | glucose dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (20/4); new base A (6/2); total (26/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGAT > minE/125410‑125541 | tttGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGCTa > 1:1073343/1‑69 (MQ=255)tttGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGCTa > 1:397131/1‑69 (MQ=255)tttGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGCTa > 1:151714/1‑69 (MQ=255) tGACCTTCCTGATTGCGACCATAGGCAGGCCAGTCCTGATCGGCAACGGGGGAGATGGCTTCTGCTggc > 1:2802582/1‑68 (MQ=255) tGACCTTCCTGATTGCGACCATAGGCAGGCCAGTCCTGATCGGCAACGGGGGAGATGGCTTCTGCTggc > 1:776173/1‑68 (MQ=255) tGACCTTCCTGATTGCGACCATAGGCAGGCCAGTCCTGATCGGCAACGGGGGAGATGGCTTCTGCTggc > 1:2395529/1‑68 (MQ=255) tGACCTTCCTGATTGCGACCATAGGCAGGCCAGTCCTGATCGGCAACGGGGGAGATGGCTTCTGCTggc > 1:1891082/1‑68 (MQ=255) tGACCTTCCTGATTGCGACCATAGGCAGGCCAGTCCTGATCGGCAACGGGGGAGATGGCTTCTGCTggc > 1:1884412/1‑68 (MQ=255) tGACCTTCCTGATTGCGACCATAGGCAGGCCAGTCCTGATCGGCAACGGGGGAGATGGCTTCTGCTgg > 1:1444255/1‑68 (MQ=255) gACCTTCCTGATTGCGACCATAGGCAGGCCAGTCCTGATCGGCAACGGGGGAGATGGCTTCTGCTggcg < 1:3258584/69‑3 (MQ=255) gACCTTCCTGATTGCGACCATAGGCAGGCCAGTCCTGATCGGCAACGGGGGAGATGGCTTCTGCTggcg < 1:949902/69‑3 (MQ=255) tACGACCATAGGCAGGCCAGTCCTGATCGGCTACGGGGGAGATAGCTTCAGCAGGTGTgg > 1:1693892/1‑60 (MQ=255) tACGACCATAGGCAGGCCAGTCCTGATCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGgcg > 1:703967/1‑68 (MQ=255) tACGACCATAGGCAGGCCAGTCCTGATCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGgcg > 1:1532830/1‑68 (MQ=255) tACGACCATAGGCAGGCCAGTCCTGATCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGgcg > 1:749228/1‑68 (MQ=255) tACGACCATAGGCAGGCCAGTCCTGATCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGgcg > 1:631306/1‑68 (MQ=255) tACGACCATAGGCAGGCCAGTCCTGATCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGgcg > 1:1917902/1‑68 (MQ=255) tACGACCATAGGCAGGCCAGTCCTGATCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGgcg > 1:1333462/1‑68 (MQ=255) tACGACCATAGGCAGGCCAGTCCTGATCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGgcg > 1:2446655/1‑68 (MQ=255) tACGACCATAGGCAGGCCAGTCCTGATCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGgcg > 1:1146608/1‑68 (MQ=255) aCGACCATAGGCAGGCCAGTCCTGATCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGgcgc > 1:1745596/1‑68 (MQ=255) gACCATAGGCAGGCCAGTCCTGATCGGCTACGGGGGAGATAGCTTCAGCAgg < 1:1627714/52‑1 (MQ=255) cAGTCCTGATCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGAtc < 1:623050/69‑1 (MQ=255) cAGTCCTGATCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGAtc < 1:1099748/69‑1 (MQ=255) aGTCCTGATCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGAtc < 1:1144812/68‑1 (MQ=255) ccTGATCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCt > 1:1915954/1‑69 (MQ=255) cTGATCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTg > 1:122985/1‑69 (MQ=255) aTCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTaa > 1:2948590/1‑48 (MQ=255) aTCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTaa > 1:401159/1‑48 (MQ=255) aTCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTaa > 1:1870494/1‑48 (MQ=255) aTCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTaa > 1:1500874/1‑48 (MQ=255) cGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGAt > 1:2094718/1‑69 (MQ=255) | TTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGATCGGCTACGGGGGAGATAGCTTCAGCAGGTGTGGCATCGGCGCTTAAGGTGCCGTTGATCTCCTGCGGAT > minE/125410‑125541 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |