| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,611,501 | A→G | 50.8% | D79D (GAT→GAC) | eutB ← | ethanolamine ammonia‑lyase, large subunit, heavy chain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,611,501 | 0 | A | G | 50.8% | 2.6 / 125.3 | 136 | D79D (GAT→GAC) | eutB | ethanolamine ammonia‑lyase, large subunit, heavy chain |
| Reads supporting (aligned to +/- strand): ref base A (44/23); new base G (30/39); total (74/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.04e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
Only 100 of 136 total aligned reads displayed.
CTCACGCAGTTCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGATTATTGCGGATGT > minE/1611444‑1611565 | cTCACGCAGTTCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGa > 1:2858829/1‑67 (MQ=255)cTCACGCAGTTCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGa > 1:1516486/1‑67 (MQ=255) tCACGCAGTTCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATc > 1:2856604/1‑69 (MQ=255) tCACGCAGTTCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATc > 1:792129/1‑69 (MQ=255) tCACGCAGTTCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATc > 1:626305/1‑69 (MQ=255) tCACGCAGTTCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATc > 1:637778/1‑69 (MQ=255) cAGTTCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGcc > 1:713560/1‑68 (MQ=255) cAGTTCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCcg > 1:1001210/1‑69 (MQ=255) cAGTTCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCcg > 1:2281395/1‑69 (MQ=255) cAGTTCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCcg > 1:2031906/1‑69 (MQ=255) cAGTTCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCcg > 1:1888368/1‑69 (MQ=255) cAGTTCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCcg > 1:1862650/1‑69 (MQ=255) cAGTTCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCcg > 1:532930/1‑69 (MQ=255) tCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGt > 1:3009566/1‑68 (MQ=255) tCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGt > 1:2073365/1‑68 (MQ=255) tCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGt > 1:2722794/1‑68 (MQ=255) tCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGt > 1:409482/1‑68 (MQ=255) tCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGt > 1:165508/1‑68 (MQ=255) tCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGTc > 1:3164064/1‑69 (MQ=255) tCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGTc > 1:16269/1‑69 (MQ=255) tCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGTc > 1:1135682/1‑69 (MQ=255) cGCTGCTGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGc > 1:201942/1‑62 (MQ=255) cGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTg > 1:1425119/1‑55 (MQ=255) cGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGTCa > 1:2428507/1‑69 (MQ=255) cGCTGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGt > 1:1803758/1‑67 (MQ=255) cGCTGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCa > 1:2299421/1‑69 (MQ=255) cGCTGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCa > 1:461474/1‑69 (MQ=255) cGCTGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCa > 1:3120558/1‑69 (MQ=255) cGCTGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCa > 1:532370/1‑69 (MQ=255) cGCTGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCa > 1:2021616/1‑69 (MQ=255) cGCTGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCa > 1:2369253/1‑69 (MQ=255) cGCTGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCa > 1:1324786/1‑69 (MQ=255) cGCTGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCa > 1:1461228/1‑69 (MQ=255) cGCTGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTAGTCCTGa > 1:7407/1‑56 (MQ=255) tGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACg < 1:2342528/68‑1 (MQ=255) tGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACg < 1:2287074/68‑1 (MQ=255) tGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACg < 1:3252365/68‑1 (MQ=255) tGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACg < 1:2624018/68‑1 (MQ=255) tGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACg < 1:1916946/68‑1 (MQ=255) tGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACg < 1:2705206/68‑1 (MQ=255) tGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACg < 1:165254/68‑1 (MQ=255) tGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACGc < 1:2402589/69‑1 (MQ=255) tGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACGc < 1:2451067/69‑1 (MQ=255) tGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACGc < 1:1330568/69‑1 (MQ=255) tGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACGc < 1:2900188/69‑1 (MQ=255) tGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACGc < 1:2896091/69‑1 (MQ=255) tGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACGc < 1:905863/69‑1 (MQ=255) tGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACGc < 1:3430491/69‑1 (MQ=255) tGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACGc < 1:381286/69‑1 (MQ=255) tGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACGc < 1:2073072/69‑1 (MQ=255) tGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACGc < 1:895294/69‑1 (MQ=255) tGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACGc < 1:178549/69‑1 (MQ=255) tGATGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACGc < 1:1302893/69‑1 (MQ=255) aTGCTCCAGTTTTTAATCTGGTTATAGGCCGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACGc < 1:1502039/67‑1 (MQ=255) gCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGTCACGCAGTc < 1:3122159/69‑1 (MQ=255) gCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGTCACGCAGTc < 1:511708/69‑1 (MQ=255) gCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGTCACGCAGTc < 1:908752/69‑1 (MQ=255) gCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGTCACGCAGTc < 1:948435/69‑1 (MQ=255) gCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGTCACGCAGTc < 1:2725426/69‑1 (MQ=255) gCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGTCACGCAGTc < 1:1455330/69‑1 (MQ=255) gCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGTCACGCAGTc < 1:1500511/69‑1 (MQ=255) gCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGTCACGCAGTc < 1:1723146/69‑1 (MQ=255) cTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGTCACGCAGTc < 1:3288447/68‑1 (MQ=255) cTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAAt < 1:774959/69‑1 (MQ=255) cTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAAt < 1:2907700/69‑1 (MQ=255) cTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAAt < 1:1905975/69‑1 (MQ=255) ccGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGattat < 1:284897/69‑1 (MQ=255) ccGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGattat < 1:857308/69‑1 (MQ=255) ccGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGattat < 1:1065049/69‑1 (MQ=255) ccGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGattat < 1:3231304/69‑1 (MQ=255) ccGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGattat < 1:2533256/69‑1 (MQ=255) ccGTTTCGTTAACGTCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGattat < 1:302705/69‑1 (MQ=255) tttCGTTAACGTCGTCCTGAATCAGCCGCGTCACGc < 1:1164744/36‑1 (MQ=255) ttCGTTAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGATTATTGCg < 1:798548/69‑1 (MQ=255) ttCGTTAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGATTATTGCg < 1:855436/69‑1 (MQ=255) ttCGTTAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGATTATTGCg < 1:2447055/69‑1 (MQ=255) tCGTTAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGATTATTGCg < 1:300744/68‑1 (MQ=255) cGTTAACGTCGTCCTGAATCAGCCGCGTGACGCAGTc > 1:101337/1‑37 (MQ=37) cGTTAACGTCGTCCTGAATCAGCCGCGTCACGCAGTc > 1:103638/1‑37 (MQ=255) cGTTAACGTCGTCCTGAATCAGCCGCGTCACGCAGTc > 1:1047765/1‑37 (MQ=255) cGTTAACGTCGTCCTGAATCAGCCGCGTCACGCAGTc > 1:2153041/1‑37 (MQ=255) cGTTAACGTCGTCCTGAATCAGCCGCGTCACGCAGTc > 1:2199401/1‑37 (MQ=255) cGTTAACGTCGTCCTGAATCAGCCGCGTCACGCAGTc > 1:2405581/1‑37 (MQ=255) cGTTAACGTCGTCCTGAATCAGCCGCGTCACGCAGTc > 1:706305/1‑37 (MQ=255) cGTTAACGTCGTCCTGAATCAGCCGCGTCACGCAGTc > 1:3097115/1‑37 (MQ=255) cGTTAACGTCGTCCTGAATCAGCCGCGTCACGCAGTc > 1:2347875/1‑37 (MQ=255) gTTAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGATTATTGCg < 1:1877139/66‑1 (MQ=255) gTTAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGATTATTGCg < 1:2725831/66‑1 (MQ=255) gTTAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGATTATTGCg < 1:847526/66‑1 (MQ=255) tAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATa > 1:1142730/1‑43 (MQ=255) tAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGATTATTGCGGATGt > 1:1058542/1‑69 (MQ=255) tAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGATTATTGCGGATGt > 1:933934/1‑69 (MQ=255) tAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGATTATTGCGGATGt > 1:1438941/1‑69 (MQ=255) tAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGATTATTGCGGATGt > 1:2697499/1‑69 (MQ=255) tAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGATTATTGCGGATGt > 1:2491949/1‑69 (MQ=255) tAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGATTATTGCGGATGt > 1:1483138/1‑69 (MQ=255) tAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGATTATTGCGGATGt > 1:3020472/1‑69 (MQ=255) tAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGATTATTGCGGATGt > 1:2124699/1‑69 (MQ=255) tAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGATTATTGCGGATGt > 1:3128572/1‑69 (MQ=255) tAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGATTATTGCGGATGt > 1:2387459/1‑69 (MQ=255) | CTCACGCAGTTCGCTGATGCTCCAGTTTTTAATCTGGTTGTAGGCCGTTTCGTTAACATCGTCCTGAATCAGCCGCGTCACGCAGTCATCTTCATAGGCAATCACCGGATTATTGCGGATGT > minE/1611444‑1611565 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |