| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,612,062 | T→C | 36.0% | D364G (GAT→GGT) ‡ | eutA ← | reactivating factor for ethanolamine ammonia lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,612,062 | 0 | T | C | 36.0% | 51.3 / 51.8 | 86 | D364G (GAT→GGT) ‡ | eutA | reactivating factor for ethanolamine ammonia lyase |
| Reads supporting (aligned to +/- strand): ref base T (24/31); new base C (10/21); total (34/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.12e-01 | |||||||||||
GCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGG > minE/1612002‑1612124 | gcggcgTAACGCACAGGCGGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc > 1:2822798/1‑69 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGGGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc < 1:2131727/69‑1 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc < 1:2666691/69‑1 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc > 1:605132/1‑69 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc > 1:2273625/1‑69 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc < 1:2379495/69‑1 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc > 1:2448026/1‑69 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc < 1:457178/69‑1 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc > 1:454207/1‑69 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc > 1:255518/1‑69 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc > 1:1098722/1‑69 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc > 1:404641/1‑69 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc > 1:39514/1‑69 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc < 1:2858563/69‑1 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc < 1:390895/69‑1 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc > 1:3286778/1‑69 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc > 1:3199334/1‑69 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc > 1:3275275/1‑69 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc < 1:720355/69‑1 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc < 1:767336/69‑1 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc < 1:2057277/69‑1 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc < 1:980380/69‑1 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc > 1:700784/1‑69 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc < 1:1511481/69‑1 (MQ=255)gcggcgTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATc > 1:2353891/1‑68 (MQ=255)gcggcgTAACGCACAGGCAGAGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc > 1:3087461/1‑69 (MQ=255) gTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTg < 1:666643/69‑1 (MQ=255) tAACGCATAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:2404313/69‑1 (MQ=255) tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:1856232/69‑1 (MQ=255) tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:163095/69‑1 (MQ=255) tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:1307234/69‑1 (MQ=255) tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:9830/69‑1 (MQ=255) tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:2579801/69‑1 (MQ=255) tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:2143613/69‑1 (MQ=255) tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:413125/69‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:3083994/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:3049725/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2968649/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:1265533/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:840527/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:3243509/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2809380/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2587846/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2766479/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:1462152/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2598080/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:257595/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:446669/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:1520207/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:1621681/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2425130/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:472156/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:474538/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:515934/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2179688/67‑1 (MQ=255) aCGCACAGGCAGCGAGCCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2279345/67‑1 (MQ=255) gCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:3387491/69‑1 (MQ=255) gCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:985722/69‑1 (MQ=255) gCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:2975654/69‑1 (MQ=255) gCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:2817081/69‑1 (MQ=255) gCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:2204798/69‑1 (MQ=255) gCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:2489156/69‑1 (MQ=255) cGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:2284128/68‑1 (MQ=255) aGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCTTGTTGc < 1:4499/68‑1 (MQ=255) ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGcc > 1:2777597/1‑68 (MQ=255) ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:202669/1‑69 (MQ=255) ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:695394/1‑69 (MQ=255) ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:610392/1‑69 (MQ=255) ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:219918/1‑69 (MQ=255) ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:1389468/1‑69 (MQ=255) ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:886534/1‑69 (MQ=255) ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:1370616/1‑69 (MQ=255) ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:1166626/1‑69 (MQ=255) ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:2949640/1‑69 (MQ=255) aGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGccccc > 1:3151785/1‑69 (MQ=255) aGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGcccc > 1:1386367/1‑68 (MQ=255) gCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:2155431/51‑1 (MQ=255) aGTTTTGGGATCAAGATCCAGCTGAATCCGCGCCTGTTGCCAGGCCCCCACCAGAt > 1:2575353/1‑56 (MQ=255) aGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAAt > 1:1098557/1‑69 (MQ=255) aGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAAt > 1:491111/1‑69 (MQ=255) aGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAAt > 1:2494811/1‑69 (MQ=255) aGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAAt > 1:2669320/1‑69 (MQ=255) aGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAAt > 1:40910/1‑69 (MQ=255) aGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAAt > 1:3398330/1‑69 (MQ=255) ttGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCggg < 1:1389875/69‑1 (MQ=255) ttGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCggg < 1:2420082/69‑1 (MQ=255) | GCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGG > minE/1612002‑1612124 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |