| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,612,073 | C→T | 44.1% | Q360Q (CAG→CAA) | eutA ← | reactivating factor for ethanolamine ammonia lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,612,073 | 0 | C | T | 44.1% | 16.6 / 53.0 | 71 | Q360Q (CAG→CAA) | eutA | reactivating factor for ethanolamine ammonia lyase |
| Reads supporting (aligned to +/- strand): ref base C (9/30); new base T (10/21); total (19/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCA > minE/1612007‑1612137 | gTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTg < 1:666643/69‑1 (MQ=255) tAACGCATAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:2404313/69‑1 (MQ=255) tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:9830/69‑1 (MQ=255) tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:2579801/69‑1 (MQ=255) tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:1307234/69‑1 (MQ=255) tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:1856232/69‑1 (MQ=255) tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:2143613/69‑1 (MQ=255) tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:163095/69‑1 (MQ=255) tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:413125/69‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:3049725/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:257595/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2179688/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2968649/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2809380/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2766479/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2598080/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2425130/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2587846/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:3083994/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:3243509/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:1621681/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:1520207/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:1462152/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:446669/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:472156/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:474538/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:515934/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:1265533/67‑1 (MQ=255) aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:840527/67‑1 (MQ=255) aCGCACAGGCAGCGAGCCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2279345/67‑1 (MQ=255) gCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:3387491/69‑1 (MQ=255) gCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:2975654/69‑1 (MQ=255) gCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:2817081/69‑1 (MQ=255) gCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:985722/69‑1 (MQ=255) gCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:2489156/69‑1 (MQ=255) gCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:2204798/69‑1 (MQ=255) cGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:2284128/68‑1 (MQ=255) aGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCTTGTTGc < 1:4499/68‑1 (MQ=255) ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGcc > 1:2777597/1‑68 (MQ=255) ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:1166626/1‑69 (MQ=255) ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:610392/1‑69 (MQ=255) ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:1370616/1‑69 (MQ=255) ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:202669/1‑69 (MQ=255) ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:695394/1‑69 (MQ=255) ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:219918/1‑69 (MQ=255) ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:2949640/1‑69 (MQ=255) ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:886534/1‑69 (MQ=255) ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:1389468/1‑69 (MQ=255) aGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGccccc > 1:3151785/1‑69 (MQ=255) aGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGcccc > 1:1386367/1‑68 (MQ=255) gCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:2155431/51‑1 (MQ=255) aGTTTTGGGATCAAGATCCAGCTGAATCCGCGCCTGTTGCCAGGCCCCCACCAGAt > 1:2575353/1‑56 (MQ=255) aGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAAt > 1:491111/1‑69 (MQ=255) aGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAAt > 1:40910/1‑69 (MQ=255) aGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAAt > 1:3398330/1‑69 (MQ=255) aGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAAt > 1:2669320/1‑69 (MQ=255) aGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAAt > 1:1098557/1‑69 (MQ=255) aGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAAt > 1:2494811/1‑69 (MQ=255) ttGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCggg < 1:2420082/69‑1 (MQ=255) ttGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCggg < 1:1389875/69‑1 (MQ=255) ccAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:607864/69‑1 (MQ=255) ccAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:1043488/69‑1 (MQ=255) ccAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:1602166/69‑1 (MQ=255) ccAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:1821658/69‑1 (MQ=255) ccAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:1909804/69‑1 (MQ=255) ccAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:2207739/69‑1 (MQ=255) ccAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:2635437/69‑1 (MQ=255) ccAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:244984/69‑1 (MQ=255) ccAGATGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:2714844/69‑1 (MQ=255) cAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:1449531/68‑1 (MQ=255) cAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:2128642/68‑1 (MQ=255) | GTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCA > minE/1612007‑1612137 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |