| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,612,205 | G→A | 84.1% | G316G (GGC→GGT) | eutA ← | reactivating factor for ethanolamine ammonia lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,612,205 | 0 | G | A | 84.1% | 90.4 / 7.4 | 56 | G316G (GGC→GGT) | eutA | reactivating factor for ethanolamine ammonia lyase |
| Reads supporting (aligned to +/- strand): ref base G (7/2); new base A (31/16); total (38/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
CGGCAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCACCGTGGCGCGCACGGTTTGCGCCGGAAACTGTACGTTCATCTCACGCAGGCGCG > minE/1612148‑1612272 | cGGCAGTTGTACGCCCTCCAGCCAGATTGTGCTGCCAGAGAGCGATAGGGTATGAGCACCCGCGCCaa > 1:3105535/1‑68 (MQ=255)cGGCAGTTGTACGCCCTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCaa > 1:327672/1‑68 (MQ=255)cGGCAGTTGTACGCCCTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCaa > 1:218769/1‑68 (MQ=255)cGGCAGTTGTACGCCCTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCaa > 1:1852602/1‑68 (MQ=255)cGGCAGTTGTACGCCCTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAAt > 1:102936/1‑69 (MQ=255)cGGCAGTTGTACGCCCTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAAt > 1:998095/1‑69 (MQ=255)cGGCAGTTGTACGCCCTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAAt > 1:866840/1‑69 (MQ=255)cGGCAGTTGTACGCCCTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAAt > 1:3334848/1‑69 (MQ=255)cGGCAGTTGTACGCCCTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAAt > 1:3152731/1‑69 (MQ=255)cGGCAGTTGTACGCCCTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAAt > 1:313294/1‑69 (MQ=255)cGGCAGTTGTACGCCCTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAAt > 1:1878216/1‑69 (MQ=255)cGGCAGTTGTACGCCCTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAAt > 1:1849652/1‑69 (MQ=255)cGGCAGTTGTACGCCCTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAAt > 1:1774492/1‑69 (MQ=255)cGGCAGTTGTACGCCCTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAAt > 1:1703795/1‑69 (MQ=255)cGGCAGTTGTACGCCCTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAAt > 1:1000425/1‑69 (MQ=255)cGGCAGTTGTACGCCCTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAAt > 1:1186498/1‑69 (MQ=255)cGGCAGTTGTACGCCCTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAAt > 1:1300854/1‑69 (MQ=255)cGGCAGTTGTACGCCCTCCAGCCAGATTGTGCTGCCAGAGAGCGAAA‑GGTATGAGCACCCGCGCCAATc > 1:1583815/1‑69 (MQ=255) cAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCAc < 1:3023099/69‑1 (MQ=255) aGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCAc < 1:2871924/68‑1 (MQ=255) gTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCACCGTgg > 1:3017057/1‑69 (MQ=255) gTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCACCGTgg > 1:764178/1‑69 (MQ=255) gTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCACCGTgg > 1:1602923/1‑69 (MQ=255) gTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCACCGTgg > 1:2911726/1‑69 (MQ=255) gTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCACCGTgg > 1:1398582/1‑69 (MQ=255) cccTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGagcacc > 1:2556952/1‑47 (MQ=255) cccTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATc > 1:1813478/1‑57 (MQ=255) cccTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTgg > 1:610329/1‑64 (MQ=255) cccTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGgc > 1:784915/1‑65 (MQ=255) cccTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGgc > 1:1758738/1‑65 (MQ=255) cccTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGgc > 1:307398/1‑65 (MQ=255) cccTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGgc > 1:403387/1‑65 (MQ=255) cccTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGgc > 1:2656726/1‑65 (MQ=255) cccTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGgc > 1:2733493/1‑65 (MQ=255) cccTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGgc > 1:425035/1‑65 (MQ=255) cccTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGgc > 1:3028572/1‑65 (MQ=255) cccTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGgc > 1:293919/1‑65 (MQ=255) cccTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGgc > 1:404915/1‑65 (MQ=255) cccTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGgc > 1:3176119/1‑65 (MQ=255) ccTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGGCGCGCa < 1:1206676/69‑1 (MQ=255) ccTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGGCGCGCa < 1:2138397/69‑1 (MQ=255) ccTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGGCGCGCa < 1:1368181/69‑1 (MQ=255) ccTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGGCGCGCa < 1:2479775/69‑1 (MQ=255) ccTCCAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGGCGCGCa < 1:863950/69‑1 (MQ=255) ccaGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGGCGCGCACgg < 1:579800/67‑1 (MQ=255) ccaGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGGCGCGCACgg < 1:346073/67‑1 (MQ=255) ccaGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGGCGCGCACgg < 1:1666298/67‑1 (MQ=255) ccaGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGGCGCGCACgg < 1:3182869/67‑1 (MQ=255) ccaGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGGCGCGCACgg < 1:1109757/67‑1 (MQ=255) ccaGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGGCGCGCACgg < 1:1399003/67‑1 (MQ=255) ccaGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGGCGCGCACgg < 1:1281533/67‑1 (MQ=255) ccaGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGGCGCGCACgg < 1:1887369/67‑1 (MQ=255) ccaGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGGCGCGCACgg < 1:3182068/67‑1 (MQ=255) ccaGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGGCGCGCACgg < 1:2232249/67‑1 (MQ=255) ccaGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGAGCACCCGCGCCAATCACCGTGGCGCGCACgg < 1:2405097/67‑1 (MQ=255) gTATGCGCGCCCGCGCCAATCACCGTGGCGCGCACGGTTTGCGCCGGAAACTGTACGTTCATCTCACGc > 1:1975564/1‑69 (MQ=255) gTATGCGCGCCCGCGCCAATCACCGTGGCGCGCACGGTTTGCGCCGGAAACTGTACGTTCATCTCACGc > 1:88357/1‑69 (MQ=255) cgcgcccgcgccAATCACCGTGGCGCGCACGGTTTGCGCCGGAAACTGTACGTTCATCTCACGCAGgcg > 1:2506186/1‑69 (MQ=255) cgcgcccgcgccAATCACCGTGGCGCGCACGGTTTGCGCCGGAAACTGTACGTTCATCTCACGCAGgcg > 1:1045077/1‑69 (MQ=255) cgcgcccgcgccAATCACCGTGGCGCGCACGGTTTGCGCCGGAAACTGTACGTTCATCTCACGCAGgcg > 1:77917/1‑69 (MQ=255) cgcgcccgcgccAATCACCGTGGCGCGCACGGTTTGCGCCGGAAACTGTACGTTCATCTCACGCAGgcg > 1:2498585/1‑69 (MQ=255) cgcgcccgcgccAATCACCGTGGCGCGCACGGTTTGCGCCGGAAACTGTACGTTCATCTCACGCAGgcg > 1:3382777/1‑69 (MQ=255) cgcgcccgcgccAATCACCGTGGCGCGCACGGTTTGCGACGGAAACTGTACGTTCATCTCACGCAGgcg > 1:1103679/1‑69 (MQ=255) cgcccgcgccAATCACCGTGGCGCGCACGGTTTGCGCCGGAAACTGTACGTTCATCTCACGCAGGcgcg < 1:1081036/69‑1 (MQ=255) gcccgcgccAATCACCGTGGCGCGCACGGTTTGCGCCGGAAACTGTACGTTCATCTCACGCAGGcgcg < 1:2980047/68‑1 (MQ=255) | CGGCAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCGCCCGCGCCAATCACCGTGGCGCGCACGGTTTGCGCCGGAAACTGTACGTTCATCTCACGCAGGCGCG > minE/1612148‑1612272 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |