| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,613,635 | C→G | 39.3% | L247L (CTG→CTC) | eutH ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,613,635 | 0 | C | G | 39.3% | 56.3 / 83.4 | 89 | L247L (CTG→CTC) | eutH | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (34/20); new base G (20/15); total (54/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGA > minE/1613567‑1613699 | acctcaccGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGc > 1:743106/1‑69 (MQ=255)acctcaccGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGc > 1:2669300/1‑69 (MQ=255) tcaccGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAgg < 1:2887955/69‑1 (MQ=255) tcaccGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAgg < 1:1524875/69‑1 (MQ=255) tcaccGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAgg < 1:1521043/69‑1 (MQ=255) tcaccGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAgg < 1:2787462/69‑1 (MQ=255) gggTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAAttt < 1:2510324/69‑1 (MQ=255) ggTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTc < 1:2914808/69‑1 (MQ=255) ggTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTc < 1:1833311/69‑1 (MQ=255) ggTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTc < 1:497472/69‑1 (MQ=255) ggTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTc < 1:2862731/69‑1 (MQ=255) ggTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTc < 1:1367232/69‑1 (MQ=255) tttATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCAc > 1:1747647/1‑69 (MQ=255) tttATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCAc > 1:1535757/1‑69 (MQ=255) gCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGc > 1:3368201/1‑69 (MQ=255) gCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGc > 1:3338354/1‑69 (MQ=255) gCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGc > 1:644159/1‑69 (MQ=255) ggCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGc < 1:156607/69‑1 (MQ=255) ggCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGc < 1:1570667/69‑1 (MQ=255) ggCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGc < 1:2239291/69‑1 (MQ=255) gCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCg > 1:1815400/1‑66 (MQ=255) aaaGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCaa > 1:2429667/1‑65 (MQ=255) aaaGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCa > 1:2822939/1‑64 (MQ=255) aaaGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAg > 1:1576962/1‑66 (MQ=255) aaaGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAg > 1:2172434/1‑66 (MQ=255) aaaGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAg > 1:1139332/1‑66 (MQ=255) aaaGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAg > 1:683212/1‑66 (MQ=255) aaaGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAg > 1:3235726/1‑66 (MQ=255) aaaGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAg > 1:2776538/1‑66 (MQ=255) aaaGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAg > 1:1371430/1‑66 (MQ=255) aaGATAGGATCCGGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCg > 1:1887195/1‑69 (MQ=255) aaGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCg > 1:3280911/1‑69 (MQ=255) aaGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCg > 1:2656518/1‑69 (MQ=255) aaGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCg > 1:1014896/1‑69 (MQ=255) aaGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCg > 1:2103817/1‑69 (MQ=255) ggATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTg > 1:3093873/1‑69 (MQ=255) ggATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTg > 1:2679847/1‑69 (MQ=255) ggATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACAGCAGCGGCAAGACCGAGGGTg > 1:1302316/1‑69 (MQ=255) tCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGAt > 1:2880336/1‑68 (MQ=255) aCCGGTGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCATTGc < 1:77495/69‑1 (MQ=255) aCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGc < 1:1308541/69‑1 (MQ=255) aCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGc < 1:3204734/69‑1 (MQ=255) aCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGc < 1:1731772/69‑1 (MQ=255) aCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGc < 1:1666463/69‑1 (MQ=255) aGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCGATGCAACGAGGAAt > 1:3145556/1‑69 (MQ=255) aGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAAt > 1:1504234/1‑69 (MQ=255) aGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAAt > 1:1482393/1‑69 (MQ=255) aGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAAt > 1:2765141/1‑69 (MQ=255) aGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAAt > 1:2251644/1‑69 (MQ=255) aGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAAt > 1:1609553/1‑69 (MQ=255) aGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAAt > 1:2850349/1‑69 (MQ=255) aGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAAt > 1:747268/1‑69 (MQ=255) aGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAAt > 1:1760879/1‑69 (MQ=255) aGTTCCAAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAAt > 1:1992875/1‑69 (MQ=255) aGCCAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCg < 1:2201496/68‑1 (MQ=255) aGCCAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCg < 1:1845437/68‑1 (MQ=255) aGCCAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCg < 1:3078543/68‑1 (MQ=255) aGCCAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCg < 1:2948097/68‑1 (MQ=255) aGCCAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCg < 1:315363/68‑1 (MQ=255) aGCCAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCg < 1:2915654/68‑1 (MQ=255) aGCCAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCg < 1:214888/68‑1 (MQ=255) aGCCAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCg < 1:996202/68‑1 (MQ=255) aGCCAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCg < 1:883437/68‑1 (MQ=255) aGCCAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCg < 1:3320123/68‑1 (MQ=255) aGCCAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCg < 1:2671450/68‑1 (MQ=255) aGCCAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCg < 1:1438765/68‑1 (MQ=255) aGCCAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCg < 1:2623792/68‑1 (MQ=255) aGCCAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCg < 1:2592016/68‑1 (MQ=255) gCCAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCg < 1:818112/67‑1 (MQ=255) ccAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAAt > 1:2787324/1‑48 (MQ=255) ccAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTgg > 1:3403810/1‑64 (MQ=255) ccAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTgg > 1:1100676/1‑64 (MQ=255) ccAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTgg > 1:1246628/1‑64 (MQ=255) ccAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTgg > 1:1258234/1‑64 (MQ=255) ccAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTgg > 1:1304176/1‑64 (MQ=255) ccAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTgg > 1:1337091/1‑64 (MQ=255) ccAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTgg > 1:1393518/1‑64 (MQ=255) ccAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTgg > 1:1550612/1‑64 (MQ=255) ccAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTgg > 1:2925615/1‑64 (MQ=255) ccAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTgg > 1:2036539/1‑64 (MQ=255) ccAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTgg > 1:2100541/1‑64 (MQ=255) ccAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTgg > 1:333717/1‑64 (MQ=255) ccAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTgg > 1:2476617/1‑64 (MQ=255) ccAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTgg > 1:3313349/1‑64 (MQ=255) ccAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTgg > 1:2576290/1‑64 (MQ=255) ccAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTgg > 1:2663391/1‑64 (MQ=255) ccAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTg > 1:997490/1‑63 (MQ=255) ccAAGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTg > 1:1733582/1‑63 (MQ=255) caaaGGAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCa > 1:807077/3‑46 (MQ=39) cAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGa < 1:642592/69‑1 (MQ=255) cAACCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGa < 1:1044140/69‑1 (MQ=255) | ACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGA > minE/1613567‑1613699 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |