| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,613,998 | A→G | 48.7% | I126I (ATT→ATC) | eutH ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,613,998 | 0 | A | G | 48.7% | ‑5.8 / 31.3 | 37 | I126I (ATT→ATC) | eutH | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (17/2); new base G (8/10); total (25/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.14e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
TCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCC > minE/1613936‑1614066 | tCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTaa > 1:73989/1‑68 (MQ=255)tCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTaa > 1:1185699/1‑68 (MQ=255)tCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTaa > 1:3251834/1‑68 (MQ=255)tCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTaa > 1:402642/1‑68 (MQ=255) cGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTaa > 1:650463/1‑67 (MQ=255) cGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTaa > 1:2253997/1‑67 (MQ=255) ccGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGtat > 1:3203276/1‑69 (MQ=255) ctggAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCAcgct > 1:2163460/3‑67 (MQ=255) ctggAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCAcgct > 1:2671353/3‑67 (MQ=255) ctggAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCAcgct > 1:2947008/3‑67 (MQ=255) ctggAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCAcgct > 1:3249737/3‑67 (MQ=255) ctggAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCAcgct > 1:2249370/3‑67 (MQ=255) ctggAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCAcgct > 1:2229887/3‑67 (MQ=255) ctggAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCAcgct > 1:3383850/3‑67 (MQ=255) ctggAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCAcgct > 1:3387428/3‑67 (MQ=255) gAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTcg > 1:459975/1‑69 (MQ=255) gAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTcg > 1:1294065/1‑69 (MQ=255) gAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTcg > 1:285834/1‑69 (MQ=255) gAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTc > 1:2157967/1‑68 (MQ=255) gAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTc > 1:1180877/1‑68 (MQ=255) gTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCACGCTGCTACGTCACCGCCCGCCAGc < 1:2518671/69‑1 (MQ=255) gTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCACGCTGCTACGTCACCGCCCGCCAGc < 1:3247162/69‑1 (MQ=255) gTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCACGCTGCTACGTCACCGCCCGCCAGc < 1:558411/69‑1 (MQ=255) gTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCACGCTGCTACGTCACCGCCCGCCAGc < 1:3073621/69‑1 (MQ=255) gTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCACGCTGCTACGTCACCGCCCGCCAGc < 1:571910/69‑1 (MQ=255) gTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCACGCTGCTACGTCACCGCCCGCCAGc < 1:2174726/69‑1 (MQ=255) gTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCACGCTGCTACGTCACCGCCCGCCAGc < 1:1040811/69‑1 (MQ=255) gTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCACGCTGCTACGTCACCGCCCGCCAGc < 1:1209605/69‑1 (MQ=255) gTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCACGCTGCTACGTCACCGCCCGCCAGc < 1:2877644/69‑1 (MQ=255) gTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCACGCTGCTACGTCACCGCCCGCCAGc < 1:2558550/69‑1 (MQ=255) tcatcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCg > 1:2077449/1‑68 (MQ=255) atcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGc < 1:1077037/38‑1 (MQ=255) atcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGctc > 1:2307923/1‑59 (MQ=255) atcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGa > 1:2425823/1‑67 (MQ=255) atcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGa > 1:2379163/1‑67 (MQ=255) atcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCAGCCAGCTCTTTGGCGa > 1:1021468/1‑67 (MQ=255) atcCACCCGAGAATTAACCCAGAGTATAGCCACGCGGc < 1:3367693/38‑1 (MQ=255) aaTTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAgccgcc < 1:2315247/69‑1 (MQ=255) aaTTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAgccgcc < 1:309726/69‑1 (MQ=255) aaTTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAgccgcc < 1:1958711/69‑1 (MQ=255) aaTTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAgccgcc < 1:1846565/69‑1 (MQ=255) aaTTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAgccgcc < 1:1285021/69‑1 (MQ=255) aaTTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAgccgcc < 1:675968/69‑1 (MQ=255) | TCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCC > minE/1613936‑1614066 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |