| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,614,031 | G→A | 40.6% | G115G (GGC→GGT) | eutH ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,614,031 | 0 | G | A | 40.6% | 24.8 / 38.1 | 59 | G115G (GGC→GGT) | eutH | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (15/20); new base A (14/10); total (29/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCG > minE/1613963‑1614094 | gAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTcg > 1:285834/1‑69 (MQ=255)gAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTcg > 1:1294065/1‑69 (MQ=255)gAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTcg > 1:459975/1‑69 (MQ=255) gTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCACGCTGCTACGTCACCGCCCGCCAGc < 1:1040811/69‑1 (MQ=255) gTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCACGCTGCTACGTCACCGCCCGCCAGc < 1:2877644/69‑1 (MQ=255) gTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCACGCTGCTACGTCACCGCCCGCCAGc < 1:3073621/69‑1 (MQ=255) gTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCACGCTGCTACGTCACCGCCCGCCAGc < 1:2558550/69‑1 (MQ=255) gTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCACGCTGCTACGTCACCGCCCGCCAGc < 1:2518671/69‑1 (MQ=255) gTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCACGCTGCTACGTCACCGCCCGCCAGc < 1:3247162/69‑1 (MQ=255) gTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCACGCTGCTACGTCACCGCCCGCCAGc < 1:2174726/69‑1 (MQ=255) gTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCACGCTGCTACGTCACCGCCCGCCAGc < 1:558411/69‑1 (MQ=255) gTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCACGCTGCTACGTCACCGCCCGCCAGc < 1:571910/69‑1 (MQ=255) gTTGGCCCCATCATCGACCCGAGGATTAACCCAGAGTATAGCCACGCTGCTACGTCACCGCCCGCCAGc < 1:1209605/69‑1 (MQ=255) tcatcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCg > 1:2077449/1‑68 (MQ=255) atcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGctc > 1:2307923/1‑59 (MQ=255) atcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGa > 1:2379163/1‑67 (MQ=255) atcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGa > 1:2425823/1‑67 (MQ=255) atcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCAGCCAGCTCTTTGGCGa > 1:1021468/1‑67 (MQ=255) aaTTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAgccgcc < 1:1958711/69‑1 (MQ=255) aaTTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAgccgcc < 1:309726/69‑1 (MQ=255) aaTTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAgccgcc < 1:2315247/69‑1 (MQ=255) aaTTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAgccgcc < 1:1846565/69‑1 (MQ=255) aaTTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAgccgcc < 1:1285021/69‑1 (MQ=255) aaTTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAgccgcc < 1:675968/69‑1 (MQ=255) tAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCat > 1:1334846/1‑69 (MQ=255) tAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCat > 1:2519782/1‑69 (MQ=255) tAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCat > 1:200344/1‑69 (MQ=255) aaCCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCat < 1:1916059/68‑1 (MQ=255) aaCCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCat < 1:2590717/68‑1 (MQ=255) aaCCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCat < 1:2001639/68‑1 (MQ=255) ccAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCg < 1:1741832/69‑1 (MQ=255) ccAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCg < 1:1063505/69‑1 (MQ=255) taGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGcc > 1:3287873/1‑67 (MQ=255) taGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGcc > 1:1929299/1‑67 (MQ=255) gCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCcagc < 1:2179449/68‑1 (MQ=255) gCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCcagc < 1:2673279/68‑1 (MQ=255) gctgCTACGTCACCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAgtgt > 1:2852751/4‑68 (MQ=255) gctgCTACGTCACCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGtt > 1:3186800/4‑69 (MQ=255) gctgCTACGTCACCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGtt > 1:78595/4‑69 (MQ=255) gctgCTACGTCACCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGtt > 1:576039/4‑69 (MQ=255) gctgCTACGTCACCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGtt > 1:465927/4‑69 (MQ=255) gctgCTACGTCACCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGtt > 1:2921390/4‑69 (MQ=255) gctgCTACGTCACCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGtt > 1:2398201/4‑69 (MQ=255) gctgCTACGTCACCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGtt > 1:2644597/4‑69 (MQ=255) gctgCTACGTCACCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGtt > 1:2569926/4‑69 (MQ=255) gctgCTACGTCACCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGtt > 1:1262839/4‑69 (MQ=255) gctgCTACGTCACCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGtt > 1:127197/4‑69 (MQ=255) gctgCTACGTCACCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGtt > 1:1373226/4‑69 (MQ=255) gctgCTACGTCACCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGtt > 1:2200367/4‑69 (MQ=255) gatgCTACGTCACCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGtt > 1:2948296/4‑69 (MQ=255) cgGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTc > 1:927272/1‑69 (MQ=255) cgGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTc > 1:2064354/1‑69 (MQ=255) cgGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTc > 1:2111492/1‑69 (MQ=255) cgGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTc > 1:233932/1‑69 (MQ=255) cgGCTAAGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTc > 1:436146/1‑69 (MQ=255) aCGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCg < 1:20274/69‑1 (MQ=255) aCGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCg < 1:1780831/69‑1 (MQ=255) aCGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCg < 1:1774027/69‑1 (MQ=255) aCGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCg < 1:2222364/69‑1 (MQ=255) aCGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCg < 1:896359/69‑1 (MQ=255) aCGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCg < 1:3001382/69‑1 (MQ=255) cGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCg < 1:3097353/68‑1 (MQ=255) | GAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCG > minE/1613963‑1614094 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |