| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,614,100 | C→T | 32.6% | S92S (TCG→TCA) | eutH ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,614,100 | 0 | C | T | 32.6% | 89.8 / 53.8 | 104 | S92S (TCG→TCA) | eutH | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (41/29); new base T (11/23); total (52/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.08e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
AGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAG > minE/1614041‑1614162 | aGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGttt > 1:906532/1‑68 (MQ=255)aGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGttt > 1:214796/1‑68 (MQ=255)aGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGttt > 1:831097/1‑68 (MQ=255)aGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGttt > 1:737851/1‑68 (MQ=255)aGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGttt > 1:2153892/1‑68 (MQ=255)aGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGttt > 1:2398029/1‑68 (MQ=255)aGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGttt > 1:2779438/1‑68 (MQ=255)aGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGttt > 1:3376136/1‑68 (MQ=255)aGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGtt > 1:1810113/1‑68 (MQ=255)aGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGtt > 1:47813/1‑68 (MQ=255) tGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCg > 1:3356604/1‑68 (MQ=255) tGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCg > 1:2337784/1‑68 (MQ=255) tGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCg > 1:2820735/1‑68 (MQ=255) tGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCg > 1:1475295/1‑68 (MQ=255) tGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCg > 1:3031460/1‑68 (MQ=255) gCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGTTTGCGCCg < 1:2846322/66‑1 (MQ=255) gCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGTTTGCGCCg < 1:2829075/66‑1 (MQ=255) gCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGTTTGCGCCg < 1:2982459/66‑1 (MQ=255) gCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGTTTGCGCCg < 1:3093649/66‑1 (MQ=255) gCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGTTTGCGCCg < 1:2656710/66‑1 (MQ=255) gCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGTTTGCGCCg < 1:1289176/66‑1 (MQ=255) gCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGTTTGCGCCg < 1:1168240/66‑1 (MQ=255) gCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGTTTGCGCCg < 1:2127355/66‑1 (MQ=255) cGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGTTTGCGCCg < 1:707125/65‑1 (MQ=255) gAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCa < 1:2849535/68‑1 (MQ=255) gAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCa < 1:480332/68‑1 (MQ=255) gaagaaGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCAttt > 1:257686/1‑68 (MQ=255) gaagaaGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCAttt > 1:1278327/1‑68 (MQ=255) gaagaaGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCAttt > 1:1786662/1‑68 (MQ=255) gccgccCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAAc > 1:2061179/1‑69 (MQ=255) gccgccCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAAc > 1:2632417/1‑69 (MQ=255) tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:2778495/1‑69 (MQ=255) tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:2274850/1‑69 (MQ=255) tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:163946/1‑69 (MQ=255) tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:765415/1‑69 (MQ=255) tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:2443083/1‑69 (MQ=255) tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:1584872/1‑69 (MQ=255) tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:273804/1‑69 (MQ=255) tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:69551/1‑69 (MQ=255) tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:2666445/1‑69 (MQ=255) tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:2830508/1‑69 (MQ=255) tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:1404567/1‑69 (MQ=255) tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:1302149/1‑69 (MQ=255) tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:3378654/1‑69 (MQ=255) tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:1253012/1‑69 (MQ=255) tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:846286/1‑69 (MQ=255) tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:1889372/1‑69 (MQ=255) tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:1189587/1‑69 (MQ=255) tCGCACGCCAGCAGTGTTCAGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:471121/1‑69 (MQ=255) gCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTAc > 1:2250776/1‑69 (MQ=255) gCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTAc > 1:609301/1‑69 (MQ=255) gCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTAc > 1:3033940/1‑69 (MQ=255) gCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTAc > 1:1715359/1‑69 (MQ=255) gCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTAc > 1:214243/1‑69 (MQ=255) gCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTAc > 1:277978/1‑69 (MQ=255) aCGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaat < 1:2253721/61‑1 (MQ=255) aCGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaat < 1:3242176/61‑1 (MQ=255) aCGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaat < 1:1328915/61‑1 (MQ=255) aCGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaat < 1:2956388/61‑1 (MQ=255) aCGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaat < 1:1523140/61‑1 (MQ=255) aCGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaat < 1:810761/61‑1 (MQ=255) aCGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaat < 1:2189482/61‑1 (MQ=255) aCGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaat < 1:2198448/61‑1 (MQ=255) aCGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaat < 1:652160/61‑1 (MQ=255) aCGCCAGCAGTGTTCCGGCGAACATCGATGGGGTAGCGCCGAGCATTTCGTAAACCGGaat < 1:544331/61‑1 (MQ=255) cGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaat < 1:1933720/60‑1 (MQ=255) cagcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:3151585/69‑1 (MQ=255) cagcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:2257211/69‑1 (MQ=255) cagcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:449970/69‑1 (MQ=255) cagcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:734549/69‑1 (MQ=255) cagcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:2599129/69‑1 (MQ=255) cagcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:2050845/69‑1 (MQ=255) cagcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:642992/69‑1 (MQ=255) cagcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:2077571/69‑1 (MQ=255) cagcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:146817/69‑1 (MQ=255) cagcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:1528004/69‑1 (MQ=255) agcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:2496352/68‑1 (MQ=255) agcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:510590/68‑1 (MQ=255) ttCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCgag > 1:1733033/1‑64 (MQ=255) ccGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACTGGAATAATTACCGGCCCGAGAACGTGa > 1:1296209/1‑69 (MQ=255) ccGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGa > 1:608274/1‑69 (MQ=255) ccGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGa > 1:1805040/1‑69 (MQ=255) ccGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGa > 1:2811604/1‑69 (MQ=255) ccGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGa > 1:1398762/1‑69 (MQ=255) ccGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGa > 1:3167011/1‑69 (MQ=255) gCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:3411360/69‑1 (MQ=255) gCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:1163801/69‑1 (MQ=255) gCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:1623227/69‑1 (MQ=255) gCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:1194167/69‑1 (MQ=255) gCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:3204710/69‑1 (MQ=255) gCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:564640/69‑1 (MQ=255) gCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:1289432/69‑1 (MQ=255) gCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:527453/69‑1 (MQ=255) gCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:1258493/69‑1 (MQ=255) gCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACCTGAGcc < 1:2053506/69‑1 (MQ=255) gAAGATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:1808836/67‑1 (MQ=255) gAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAg < 1:2760370/69‑1 (MQ=255) gAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAg < 1:114486/69‑1 (MQ=255) gAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAg < 1:2248000/69‑1 (MQ=255) tCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:1218089/62‑1 (MQ=255) | AGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAG > minE/1614041‑1614162 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |