| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,614,459 | T→C | 60.6% | intergenic (‑84/+133) | eutH ← / ← eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,614,459 | 0 | T | C | 60.6% | 4.4 / 22.4 | 38 | intergenic (‑84/+133) | eutH/eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Reads supporting (aligned to +/- strand): ref base T (7/8); new base C (11/12); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGC > minE/1614393‑1614526 | tgttgttATTTATTGGAGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTgg < 1:1333270/68‑1 (MQ=255)aGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTgg < 1:90893/69‑1 (MQ=255)aGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTg > 1:3272130/1‑68 (MQ=255)aGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTg > 1:2320274/1‑68 (MQ=255) ctGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCCCAGGCCGGATAAGAcgcgtc < 1:708654/68‑3 (MQ=11) ctGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCCCAGGCCGGATAAGAcgcgtc < 1:1128360/68‑3 (MQ=11) ctGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCCCAGGCCGGATAAGAcgcgtc < 1:540891/68‑3 (MQ=11) ctGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCCCAGGCCGGATAAGAcgcgtc < 1:531162/68‑3 (MQ=11) ctGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCCCAGGCCGGATAAGAcgcgtc < 1:1565751/68‑3 (MQ=11) ctGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCCCAGGCCGGATAAGAcgcgtc < 1:1650750/68‑3 (MQ=11) ctGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCCCAGGCCGGATAAGAcgcgtc < 1:3386435/68‑3 (MQ=11) ctGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCCCAGGCCGGATAAGAcgcgtc < 1:17926/68‑3 (MQ=11) ctGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCCCAGGCCGGATAAGAcgcgtc < 1:285646/68‑3 (MQ=11) ctGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCCCAGGCCGGATAAGAcgcgtc < 1:2595707/68‑3 (MQ=11) ctGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCCCAGGCCGGATAAGAcgcgtc < 1:2097785/68‑3 (MQ=11) tGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCCCAGGCCGGATTAGAcgcgtc < 1:1081311/68‑3 (MQ=14) ggCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:622205/68‑1 (MQ=40) ggCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:2872473/68‑1 (MQ=40) ggCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:1472472/68‑1 (MQ=40) cGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAg < 1:1049348/68‑1 (MQ=40) cGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAg < 1:1120539/68‑1 (MQ=40) cGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAg < 1:1295125/68‑1 (MQ=40) aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCa > 1:2472302/1‑69 (MQ=35) aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCa > 1:1952061/1‑69 (MQ=35) cGCCCTACATGTGCAATCCCGTAGGCCAGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcgc > 1:3384355/1‑68 (MQ=255) cGCCCTACATGTGCAATCCCGTAGGCCAGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcgc > 1:865918/1‑68 (MQ=255) cGCCCTACATGTGCAATCCCGTAGGCCAGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcgc > 1:509369/1‑68 (MQ=255) cGCCCTACATGTGCAATCCCGTAGGCCAGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcgc > 1:350298/1‑68 (MQ=255) cGCCCTACATGTGCAATCCCGTAGGCCAGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcgc > 1:2126322/1‑68 (MQ=255) cGCCCTACATGTGCAATCCCGTAGGCCAGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcgc > 1:3267569/1‑68 (MQ=255) cGCCCTACATGTGCAATCCCGTAGGCCAGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcgc > 1:2949161/1‑68 (MQ=255) cGCCCTACATGTGCAATCCCGTAGGCCAGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcgc > 1:2717019/1‑68 (MQ=255) cGCCCTACATGTGCAATCCCGTAGGCCAGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcgc > 1:2075500/1‑68 (MQ=255) cGCCCTACATGTGCAATCCCGTAGGCCAGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcgc > 1:2466883/1‑68 (MQ=255) cGCCCTACATGTGCAATCCAGTAGGCCAGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcgc > 1:1772299/1‑68 (MQ=17) gCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGcc > 1:456507/1‑59 (MQ=255) gCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGcc > 1:1873803/1‑59 (MQ=255) gCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGcc > 1:869389/1‑59 (MQ=255) tGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGc < 1:2321913/68‑1 (MQ=255) | AGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGC > minE/1614393‑1614526 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |