| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,614,832 | T→A | 57.7% | E316D (GAA→GAT) | eutG ← | predicted alcohol dehydrogenase in ethanolamine utilization |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,614,832 | 0 | T | A | 57.7% | 25.3 / 54.8 | 59 | E316D (GAA→GAT) | eutG | predicted alcohol dehydrogenase in ethanolamine utilization |
| Reads supporting (aligned to +/- strand): ref base T (12/13); new base A (13/21); total (25/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCGTTAATAGCGTCACGATCGTCGGATTTTTTAGTTCGCAGTGCCCGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGGCAGCAACA > minE/1614766‑1614878 | cgcgTTAATAGCGTCACGATCGTCGGATTTTTTAGTTCGCAGTGCCCGACCAATCTGACTAAAGCGtt < 1:3216040/68‑1 (MQ=255)cgcgTTAATAGCGTCACGATCGTCGGATTTTTTAGTTCGCAGTGCCCGACCAATCTGACTAAAGCGtt < 1:2402344/68‑1 (MQ=255)cgcgTTAATAGCGTCACGATCGTCGGATTTTTTAGTTCGCAGTGCCCGACCAATCTGACTAAAGCGtt < 1:1957400/68‑1 (MQ=255)cgcgTTAATAGCGTCACGATCGTCGGATTTTTTAGTTCGCAGTGCCCGACCAATCTGACTAAAGCGtt < 1:1573386/68‑1 (MQ=255) cACGATCGTCGGATTTTTTAGTTCGCAGCGCCCGACCAATCTGACTAAACCGATCACGACAAACCATcc > 1:2630033/1‑69 (MQ=255) cACGATCGTCGGATTTTTTAGTTCGCAGCGCCCGACCAATCTGACTAAACCGATCACGACAAACCATcc > 1:996341/1‑69 (MQ=255) cACGATCGTCGGATTTTTTAGTTCGCAGCGCCCGACCAATCTGACTAAACCGATCACGACAAACCATcc > 1:832287/1‑69 (MQ=255) cACGATCGTCGGATTTTTTAGTTCGCAGCGCCCGACCAATCTGACTAAACCGATCACGACAAACCATcc > 1:788178/1‑69 (MQ=255) cACGATCGTCGGATTTTTTAGTTCGCAGCGCCCGACCAATCTGACTAAACCGATCACGACAAACCATcc > 1:3284152/1‑69 (MQ=255) cACGATCGTCGGATTTTTTAGTTCGCAGCGCCCGACCAATCTGACTAAACCGATCACGACAAACCATcc > 1:307836/1‑69 (MQ=255) cACGATCGTCGGATTTTTTAGTTCGCAGCGCCCGACCAATCTGACTAAACCGATCACGACAAACCATcc > 1:3062612/1‑69 (MQ=255) cACGATCGTCGGATTTTTTAGTTCGCAGCGCCCGACCAATCTGACTAAACCGATCACGACAAACCATcc > 1:2499816/1‑69 (MQ=255) cACGATCGTCGGATTTTTTAGTTCGCAGCGCCCGACCAATCTGACTAAACCGATCACGACAAACCATcc > 1:2291892/1‑69 (MQ=255) cACGATCGTCGGATTTTTTAGTTCGCAGCGCCCGACCAATCTGACTAAACCGATCACGACAAACCATcc > 1:2130547/1‑69 (MQ=255) cACGATCGTCGGATTTTTTAGTTCGCAGCGCCCGACCAATCTGACTAAACCGATCACGACAAACCATcc > 1:1477823/1‑69 (MQ=255) cACGATCGTCGGATTTTTTAGTTCGCAGCGCCCGACCAATCTGACTAAACCGATCACGACAAACCATcc > 1:1264599/1‑69 (MQ=255) cACGATCGTCGGATTTTTTAGTTCGCAGCGCCCGACCAATCTGACTAAACCGATCACGACAAACCATcc > 1:1549951/1‑69 (MQ=255) tGCCCGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTa > 1:1070808/1‑46 (MQ=255) tGCCCGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATGACCGTTGGCAGCaa < 1:1569114/69‑1 (MQ=255) tGCCCGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGGCAGCaa > 1:928152/1‑69 (MQ=255) tGCCCGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGGCAGCaa > 1:2739105/1‑69 (MQ=255) tGCCCGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGGCAGCaa < 1:2623201/69‑1 (MQ=255) tGCCCGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGGCAGCaa > 1:2828117/1‑69 (MQ=255) tGCCCGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGGCAGCaa < 1:311930/69‑1 (MQ=255) tGCCCGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGGCAGCaa > 1:3278712/1‑69 (MQ=255) tGCCCGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGGCAGCaa < 1:3337290/69‑1 (MQ=255) tGCCCGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGGCAGCaa > 1:1614215/1‑69 (MQ=255) tGCCCGA‑CAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGGCAGCAAc > 1:2997834/1‑69 (MQ=255) cccGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGgcagc < 1:1439596/65‑1 (MQ=255) cccGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGgcagc < 1:305878/65‑1 (MQ=255) cccGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGgcagc < 1:2840055/65‑1 (MQ=255) cccGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGgcagc < 1:480666/65‑1 (MQ=255) cccGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGgcagc < 1:1205614/65‑1 (MQ=255) cccGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGgcagc < 1:2747598/65‑1 (MQ=255) cccGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGgcagc < 1:1607465/65‑1 (MQ=255) cccGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGgcagc < 1:2214978/65‑1 (MQ=255) cccGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGGCAGCAACa > 1:3073772/1‑69 (MQ=255) cccGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGGCAGCAACa > 1:555080/1‑69 (MQ=255) cccGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGGCAGCAACa > 1:397220/1‑69 (MQ=255) cccGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGGCAGCAACa > 1:1556207/1‑69 (MQ=255) cccGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGGCAGCAACa > 1:3306212/1‑69 (MQ=255) cccaCCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGgcagc < 1:1649632/61‑1 (MQ=255) gACCAATCTGACTAAACCGATCACGACAAACCATCCGGTTAAATTcc < 1:3286119/47‑1 (MQ=39) gACCAATCTGACTAAACCGATCACGACAAACCATCCGGTTAAATTcc < 1:1109031/47‑1 (MQ=39) gACCAATCTGACTAAACCGATCACGACAAACCATCCGGTTAAATTcc < 1:1586328/47‑1 (MQ=39) gACCAATCTGACTAAACCGATCACGACAAACCATCCGGTTAAATTcc < 1:917660/47‑1 (MQ=39) gACCAATCTGACTAAACCGATCACGACAAACCATCCGGTTAAATTcc < 1:1647013/47‑1 (MQ=39) gACCAATCTGACTAAACCGATCACGACAAACCATCCGGTTAAATTcc < 1:1210828/47‑1 (MQ=39) gACCAATCTGACTAAACCGATCACGACAAACCATCCGGTTAAATTcc < 1:524678/47‑1 (MQ=39) gACCAATCTGACTAAACCGATCACGACAAACCATCCGGTTAAATTcc < 1:2006790/47‑1 (MQ=39) gACCAATCTGACTAAACCGATCACGACAAACCATCCGGTTAAATTcc < 1:3299799/47‑1 (MQ=39) gACCAATCTGACTAAACCGATCACGACAAACCATCCGGTTAAATTcc < 1:3292330/47‑1 (MQ=39) gACCAATCTGACTAAACCGATCACGACAAACCATCCGGTTAAATTcc < 1:266981/47‑1 (MQ=39) gACCAATCTGACTAAACCGATCACGACAAACCATCCGGTTAAATTcc < 1:2166080/47‑1 (MQ=39) gACCAATCTGACTAAACCGATCACGACAAACCATCCGGTTAAATTcc < 1:128789/47‑1 (MQ=39) gACCAATCTGACTAAACCGATCACGACAAACCATCCGGTTAAATTcc < 1:31785/47‑1 (MQ=39) gACCAATCTGACTAAACCGATCACGACAAACCATCCGGTTAAATTcc < 1:2175269/47‑1 (MQ=39) gACCAATCTGACTAAACCGATCACGACAAACCATCCGGTTAAATTcc < 1:3116640/47‑1 (MQ=39) gACCAATCTGACTAAACCGATCACGACAAACCATCCGGTTAAATTcc < 1:1339114/47‑1 (MQ=39) gACCAATCTGACTAAACCGATCACGACAAACCATCCGGTTAAATTcc < 1:1517011/47‑1 (MQ=39) gACCAATCTGACTAAACCGATCACGACAAACCATCCGGTTAAATTcc < 1:2441626/47‑1 (MQ=39) gACCAATCTGACTAAACCGATCACGACAAACCATCCGGTTAAATTcc < 1:2493490/47‑1 (MQ=39) gACCAATCTGACTAAACCGATCACGACAAACCATCCGGTTAAATTca < 1:2182437/47‑2 (MQ=38) ttCACGACAAACCATCCGGTTAAATTCCATCACCGTTGGCAGCaa < 1:3333050/45‑1 (MQ=255) | CGCGTTAATAGCGTCACGATCGTCGGATTTTTTAGTTCGCAGTGCCCGACCAATCTGACTAAAGCGTTCACGACAAACCATCCGGTTAAATTCCATCACCGTTGGCAGCAACA > minE/1614766‑1614878 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |