| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,616,492 | G→A | 65.3% | T38T (ACC→ACT) | eutJ ← | predicted chaperonin, ethanolamine utilization protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,616,492 | 0 | G | A | 65.3% | 10.8 / 9.2 | 23 | T38T (ACC→ACT) | eutJ | predicted chaperonin, ethanolamine utilization protein |
| Reads supporting (aligned to +/- strand): ref base G (6/2); new base A (2/13); total (8/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.24e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGTCTGGTTACACAG > minE/1616431‑1616554 | aGTCGAGGCATACCGCCACCGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAAGTg > 1:3184739/1‑65 (MQ=255)aGTCGAGGCATACCGCCACCGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAAGTg > 1:2480433/1‑65 (MQ=255) ccGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCa > 1:692588/1‑69 (MQ=255) ccGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCa > 1:1612329/1‑69 (MQ=255) tGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGga < 1:1901026/69‑1 (MQ=255) gcgGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGt > 1:1128233/1‑69 (MQ=255) gcgGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGt > 1:372783/1‑69 (MQ=255) gcgGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGt > 1:1724607/1‑69 (MQ=255) gcgGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGt > 1:1119644/1‑69 (MQ=255) caTCGCAAGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTAcaca < 1:2071188/69‑1 (MQ=255) caTCGCAAGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTAcaca < 1:2111584/69‑1 (MQ=255) caTCGCAAGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTAcaca < 1:2480323/69‑1 (MQ=255) caTCGCAAGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTAcaca < 1:156069/69‑1 (MQ=255) caTCGCAAGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTAcaca < 1:3010285/69‑1 (MQ=255) caTCGCAAGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTAcaca < 1:3037729/69‑1 (MQ=255) caTCGCAAGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTAcaca < 1:3157800/69‑1 (MQ=255) caTCGCAAGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTAcaca < 1:1275880/69‑1 (MQ=255) caTCGCAAGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTAcaca < 1:3203010/69‑1 (MQ=255) caTCGCAAGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTAcaca < 1:3205924/69‑1 (MQ=255) caTCGCAAGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTAcaca < 1:1179188/69‑1 (MQ=255) caTCGCAAGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTAcaca < 1:846060/69‑1 (MQ=255) aTCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGTCTGGTTACACAg < 1:741766/69‑1 (MQ=255) aTCGCAAGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTAcaca < 1:3382719/68‑1 (MQ=255) | AGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGTCTGGTTACACAG > minE/1616431‑1616554 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |