Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,616,754 G→A 100% C422C (TGC→TGT eutE ← predicted aldehyde dehydrogenase, ethanolamine utilization protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,616,7540GA100.0% 42.3 / NA 19C422C (TGC→TGTeutEpredicted aldehyde dehydrogenase, ethanolamine utilization protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/19);  total (0/19)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTC  >  minE/1616748‑1616816
      |                                                              
ggCAATACACGGTCCGTTCTTAACGAAAATGCTGGTATCAATGGCGTTCGCCATCTGGTTCATGTTTTc  <  1:2747132/69‑1 (MQ=255)
ggCAATACACGGTCCGTTCTTAACGAAAATGCTGGTATCAATGGCGTTCGCCATCTGGTTCATGTTTTc  <  1:878893/69‑1 (MQ=255)
ggCAATACACGGTCCGTTCTTAACGAAAATGCTGGTATCAATGGCGTTCGCCATCTGGTTCATGTTTTc  <  1:874901/69‑1 (MQ=255)
ggCAATACACGGTCCGTTCTTAACGAAAATGCTGGTATCAATGGCGTTCGCCATCTGGTTCATGTTTTc  <  1:741729/69‑1 (MQ=255)
ggCAATACACGGTCCGTTCTTAACGAAAATGCTGGTATCAATGGCGTTCGCCATCTGGTTCATGTTTTc  <  1:478095/69‑1 (MQ=255)
ggCAATACACGGTCCGTTCTTAACGAAAATGCTGGTATCAATGGCGTTCGCCATCTGGTTCATGTTTTc  <  1:3383692/69‑1 (MQ=255)
ggCAATACACGGTCCGTTCTTAACGAAAATGCTGGTATCAATGGCGTTCGCCATCTGGTTCATGTTTTc  <  1:312068/69‑1 (MQ=255)
ggCAATACACGGTCCGTTCTTAACGAAAATGCTGGTATCAATGGCGTTCGCCATCTGGTTCATGTTTTc  <  1:3112939/69‑1 (MQ=255)
ggCAATACACGGTCCGTTCTTAACGAAAATGCTGGTATCAATGGCGTTCGCCATCTGGTTCATGTTTTc  <  1:2938884/69‑1 (MQ=255)
ggCAATACACGGTCCGTTCTTAACGAAAATGCTGGTATCAATGGCGTTCGCCATCTGGTTCATGTTTTc  <  1:2802984/69‑1 (MQ=255)
ggCAATACACGGTCCGTTCTTAACGAAAATGCTGGTATCAATGGCGTTCGCCATCTGGTTCATGTTTTc  <  1:1024550/69‑1 (MQ=255)
ggCAATACACGGTCCGTTCTTAACGAAAATGCTGGTATCAATGGCGTTCGCCATCTGGTTCATGTTTTc  <  1:2723742/69‑1 (MQ=255)
ggCAATACACGGTCCGTTCTTAACGAAAATGCTGGTATCAATGGCGTTCGCCATCTGGTTCATGTTTTc  <  1:2704950/69‑1 (MQ=255)
ggCAATACACGGTCCGTTCTTAACGAAAATGCTGGTATCAATGGCGTTCGCCATCTGGTTCATGTTTTc  <  1:2674300/69‑1 (MQ=255)
ggCAATACACGGTCCGTTCTTAACGAAAATGCTGGTATCAATGGCGTTCGCCATCTGGTTCATGTTTTc  <  1:261553/69‑1 (MQ=255)
ggCAATACACGGTCCGTTCTTAACGAAAATGCTGGTATCAATGGCGTTCGCCATCTGGTTCATGTTTTc  <  1:2452537/69‑1 (MQ=255)
ggCAATACACGGTCCGTTCTTAACGAAAATGCTGGTATCAATGGCGTTCGCCATCTGGTTCATGTTTTc  <  1:2098252/69‑1 (MQ=255)
ggCAATACACGGTCCGTTCTTAACGAAAATGCTGGTATCAATGGCGTTCGCCATCTGGTTCATGTTTTc  <  1:1771798/69‑1 (MQ=255)
ggCAATACACGGTCCGTTCTTAACGAAAATGCTGGTATCAATGGCGTTCGCCATCTGGTTCATGTTTTc  <  1:1362839/69‑1 (MQ=255)
      |                                                              
GGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTC  >  minE/1616748‑1616816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: