Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,616,820 G→A 42.2% N400N (AAC→AAT eutE ← predicted aldehyde dehydrogenase, ethanolamine utilization protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,616,8200GA42.2% 24.3 / 50.5 71N400N (AAC→AATeutEpredicted aldehyde dehydrogenase, ethanolamine utilization protein
Reads supporting (aligned to +/- strand):  ref base G (25/16);  new base A (14/16);  total (39/32)
Fisher's exact test for biased strand distribution p-value = 3.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

CACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGCTAGCGCAATGG  >  minE/1616755‑1616884
                                                                 |                                                                
cACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGtt                                                                >  1:2342863/1‑68 (MQ=255)
cACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTg                                                               >  1:1252758/1‑69 (MQ=255)
cACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTg                                                               >  1:2836044/1‑69 (MQ=255)
cACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTg                                                               >  1:2596444/1‑69 (MQ=255)
      ccGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAg                                                         >  1:569351/1‑69 (MQ=255)
      ccGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAg                                                         >  1:894611/1‑69 (MQ=255)
      ccGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAg                                                         >  1:1009141/1‑69 (MQ=255)
       cGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAg                                                         >  1:2975501/1‑68 (MQ=255)
       cGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAg                                                         >  1:2030139/1‑68 (MQ=255)
       cGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAg                                                         >  1:409423/1‑68 (MQ=255)
               aCGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTgcc                                                >  1:2114002/1‑69 (MQ=255)
               aCGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTgcc                                                >  1:1740/1‑69 (MQ=255)
               aCGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTgcc                                                >  1:406291/1‑69 (MQ=255)
               aCGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTgcc                                                >  1:42080/1‑69 (MQ=255)
                           gTATCAATGGCGTTCGCCATCTGGTTCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTgtggtg                                     >  1:2063560/1‑68 (MQ=255)
                           gTATCAATGGCGTTCGCCATCTGGTTCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTgtggtg                                     >  1:410201/1‑68 (MQ=255)
                           gTATCAATGGCGTTCGCCATCTGGTTCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTgtggtg                                     >  1:2583376/1‑68 (MQ=255)
                           gTATCAATGGCGTTCGCCATCTGGTTCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTgtggtg                                     >  1:353609/1‑68 (MQ=255)
                           gTATCAATGGCGTTCGCCATCTGGTTCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTgtggtg                                     >  1:59157/1‑68 (MQ=255)
                           gTATCAATGGCGTTCGCCATCTGGTTCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTgtggtg                                     >  1:2660220/1‑68 (MQ=255)
                           gTATCAATGGCGTTCGCCATCTGGTTCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTgtggtg                                     >  1:639393/1‑68 (MQ=255)
                           gTATCAATGGCGTTCGCCATCTGGTTCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTgtggtg                                     >  1:1435990/1‑68 (MQ=255)
                           gTATCAATGGCGTTCGCCATCTGGTTCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTgtggtg                                     >  1:2765657/1‑68 (MQ=255)
                           gTATCAATGGCGTTCGCCATCTGGTTCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTgtggtg                                     >  1:3025239/1‑68 (MQ=255)
                           gTATCAATGGCGTTCGCCATCTGGTTCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTgtggtg                                     >  1:133711/1‑68 (MQ=255)
                           gTATCAATGGCGTTCGCCATCTGGTTCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTgtggtg                                     >  1:869154/1‑68 (MQ=255)
                           gTATCAATGGCGTTCGCCATCTGGTTCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTgtggt                                      >  1:306980/1‑67 (MQ=255)
                           gTATCAATGGCGTTCGCCATCTGGTTCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTgtggt                                      >  1:899438/1‑67 (MQ=255)
                              tCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTg                                                       >  1:2381292/1‑47 (MQ=255)
                                         cGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAg                      >  1:2458952/1‑69 (MQ=255)
                                         cGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAg                      >  1:366100/1‑69 (MQ=255)
                                         cGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAg                      >  1:2058865/1‑69 (MQ=255)
                                         cGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAg                      >  1:1885287/1‑69 (MQ=255)
                                         cGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAg                      >  1:1849369/1‑69 (MQ=255)
                                         cGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAg                      >  1:1352433/1‑69 (MQ=255)
                                              tCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCa                 <  1:1577152/69‑1 (MQ=255)
                                                tGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCAcc               >  1:3325775/1‑69 (MQ=255)
                                                tGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCAc                >  1:1384478/1‑68 (MQ=255)
                                                  gTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCAcc               >  1:645615/1‑67 (MQ=255)
                                                   ttCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGc             <  1:2752539/68‑1 (MQ=255)
                                                    tCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCAcc               <  1:3178746/65‑1 (MQ=255)
                                                    tCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCAcc               <  1:1197080/65‑1 (MQ=255)
                                                    tCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCAcc               <  1:1908690/65‑1 (MQ=255)
                                                    tCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCAcc               <  1:3359745/65‑1 (MQ=255)
                                                    tCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCAcc               <  1:877617/65‑1 (MQ=255)
                                                    tCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCAcc               <  1:588470/65‑1 (MQ=255)
                                                    tCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCAcc               <  1:1219829/65‑1 (MQ=255)
                                                    tCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCAcc               <  1:2007521/65‑1 (MQ=255)
                                                    tCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCAcc               <  1:782572/65‑1 (MQ=255)
                                                    tCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCAcc               <  1:747710/65‑1 (MQ=255)
                                                    tCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCAcc               <  1:2045029/65‑1 (MQ=255)
                                                    tCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCAcc               <  1:532795/65‑1 (MQ=255)
                                                    tCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCAcc               <  1:477354/65‑1 (MQ=255)
                                                    tCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGc             <  1:1383227/67‑1 (MQ=255)
                                                    tCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGc             <  1:1054444/67‑1 (MQ=255)
                                                    tCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGc             <  1:698810/67‑1 (MQ=255)
                                                    tCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGc             <  1:821680/67‑1 (MQ=255)
                                                    tCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGc             <  1:869749/67‑1 (MQ=255)
                                                    tCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGc             <  1:893212/67‑1 (MQ=255)
                                                    tCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGc             <  1:2821562/67‑1 (MQ=255)
                                                    tCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGc             <  1:1769986/67‑1 (MQ=255)
                                                    tCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGc             <  1:2010858/67‑1 (MQ=255)
                                                    tCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGc             <  1:2111044/67‑1 (MQ=255)
                                                    tCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGc             <  1:399423/67‑1 (MQ=255)
                                                    tCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGc             <  1:2513729/67‑1 (MQ=255)
                                                    tCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGc             <  1:3353209/67‑1 (MQ=255)
                                                    tCATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGc             <  1:2591534/67‑1 (MQ=255)
                                                     cATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCAcc               <  1:2366646/64‑1 (MQ=255)
                                                     cATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCAcc               <  1:2711359/64‑1 (MQ=255)
                                                     cATGTTTTCGATATTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGc             <  1:2117294/66‑1 (MQ=255)
                                                             cGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGCTAGCGCAATgg  >  1:3390997/1‑69 (MQ=255)
                                                                 |                                                                
CACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGCTAGCGCAATGG  >  minE/1616755‑1616884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: