| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,619,764 | C→T | 51.4% | E4K (GAA→AAA) | eutI ← | predicted phosphotransacetylase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,619,764 | 0 | C | T | 51.4% | ‑2.5 / 57.2 | 65 | E4K (GAA→AAA) | eutI | predicted phosphotransacetylase subunit |
| Reads supporting (aligned to +/- strand): ref base C (21/10); new base T (23/10); total (45/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.89e-01 | |||||||||||
CGTTGGTCTAACGCATCCGGAAAAACCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTAGCGGCTGCTTC > minE/1619697‑1619824 | cGTTGGTCTAACGCATCCGGAAAAACCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTc > 1:55290/1‑68 (MQ=255) tAACGCATCCGGAAAAACCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCa > 1:170155/1‑68 (MQ=255) tAACGCATCCGGAAAAACCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCAt > 1:83505/1‑69 (MQ=255) tAACGCATCCGGAAAAACCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCAt > 1:2436956/1‑69 (MQ=255) tAACGCATCCGGAAAAACCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCAt > 1:814201/1‑69 (MQ=255) tAACGCATCCGGAAAAACCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCAt > 1:2522258/1‑69 (MQ=255) tAACGCATCCGGAAAAACCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCAt > 1:808646/1‑69 (MQ=255) tAACGCATCCGGAAAAACCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCAt > 1:166041/1‑69 (MQ=255) cGGAAAAACCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTct > 1:2658405/1‑68 (MQ=255) cGGAAAAACCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTcc > 1:2325388/1‑69 (MQ=255) cGGAAAAACCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTcc > 1:2321470/1‑69 (MQ=255) cGGAAAAACCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTcc > 1:230625/1‑69 (MQ=255) aCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGt > 1:2566387/1‑68 (MQ=255) aCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGt > 1:816210/1‑68 (MQ=255) aCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTc > 1:3092969/1‑69 (MQ=255) aCCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTc > 1:508298/1‑69 (MQ=255) cACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCgt > 1:1095444/1‑69 (MQ=255) cACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCgt > 1:2360071/1‑69 (MQ=255) gggcgCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTgg < 1:2130220/66‑1 (MQ=255) gggcgCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTgg < 1:3335605/66‑1 (MQ=255) gggcgCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTgg < 1:3182965/66‑1 (MQ=255) gggcgCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTgg < 1:3156240/66‑1 (MQ=255) gggcgCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTgg < 1:2801519/66‑1 (MQ=255) gggcgCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTgg < 1:1890847/66‑1 (MQ=255) gggcgCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTgg < 1:2634814/66‑1 (MQ=255) gggcgCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTgg < 1:1920895/66‑1 (MQ=255) gggcgCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTgg < 1:828370/66‑1 (MQ=255) aCGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTgg < 1:2869757/55‑1 (MQ=255) gCCAGTTCACGACAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTa < 1:749619/69‑1 (MQ=255) gCCAGTTCACGACAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTa < 1:638972/69‑1 (MQ=255) gCCAGTTCACGACAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTa < 1:3292201/69‑1 (MQ=255) gCCAGTTCACGACAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTa < 1:3187067/69‑1 (MQ=255) gCCAGTTCACGACAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTa < 1:3122055/69‑1 (MQ=255) gCCAGTTCACGACAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTa < 1:1683687/69‑1 (MQ=255) gCCAGTTCACGACAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTa < 1:2497068/69‑1 (MQ=255) gCCAGTTCACGACAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTa < 1:2557092/69‑1 (MQ=255) gCCAGTTCACGACAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTa < 1:2546905/69‑1 (MQ=255) gCCAGTTCACGACAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTa < 1:2521781/69‑1 (MQ=255) tCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCgttgt > 1:1551174/1‑44 (MQ=255) tCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCtt > 1:2049372/1‑55 (MQ=255) tCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTAGCgg > 1:2073878/1‑67 (MQ=255) tCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTAGCGgct > 1:2466591/1‑69 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCgttgt > 1:199845/1‑39 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCgttgt > 1:2805339/1‑39 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCgttgt > 1:258583/1‑39 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCgtt > 1:2472485/1‑37 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCgt > 1:2036748/1‑36 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCGttgttg > 1:1951865/1‑41 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGa > 1:1583762/1‑43 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGAt > 1:2922875/1‑44 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTg > 1:3192478/1‑47 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTg > 1:2303241/1‑47 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTg > 1:1975812/1‑47 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCAcc > 1:2579861/1‑54 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCAcc > 1:2907775/1‑54 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTAg > 1:456883/1‑59 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTAg > 1:2049847/1‑59 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTAGCGgc > 1:777136/1‑63 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTAGCGGCTGCTTc > 1:1458817/1‑69 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTAGCGGCTGCTTc > 1:3415807/1‑69 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTAGCGGCTGCTTc > 1:2704669/1‑69 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTAGCGGCTGCTTc > 1:2507453/1‑69 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTAGCGGCTGCTTc > 1:2406855/1‑69 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTAGCGGCTGCTTc > 1:1948022/1‑69 (MQ=255) aCAACGTTTAATAATCATTGCGTTTCCCCCAGTCGTGGTTggt > 1:2350056/1‑42 (MQ=38) aCAACGTTAAATAATCATTGCGTTTCCCCCAGTCGttgttg > 1:989338/1‑41 (MQ=255) | CGTTGGTCTAACGCATCCGGAAAAACCACTCTGGCGGGCGCTCGCAACGCCAGTTCACGACAACGTTCAATAATCATTGCGTTTCCCCCAGTCGTTGTTGGATCTGCTTCACCGTTAGCGGCTGCTTC > minE/1619697‑1619824 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |