| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,620,888 | A→G | 52.1% | S128S (AGT→AGC) | eutQ ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,620,888 | 0 | A | G | 52.1% | 0.9 / 44.9 | 55 | S128S (AGT→AGC) | eutQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (23/3); new base G (10/18); total (34/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.04e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01 | |||||||||||
CAGTCAAGCCCACGCAATGCGGCTCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGC > minE/1620833‑1620937 | cAGTCAAGCCCACGCAATGCGGCTCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCatcaatca > 1:444957/1‑69 (MQ=255) ccACGCAATGCGGCTCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATgc < 1:1287546/69‑1 (MQ=255) ccACGCAATGCGGCTCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATgc < 1:302631/69‑1 (MQ=255) gCAATGCGGCTCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATgccgcc < 1:1715299/69‑1 (MQ=255) gCGGTTGTGCGCCGTCGAAACGACCAAACTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGc < 1:2991575/69‑1 (MQ=255) gCGGTTGTGCGCCGTCGAAACGACCAAACTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGc < 1:2393324/69‑1 (MQ=255) gCGGTTGTGCGCCGTCGAAACGACCAAACTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGc < 1:3293319/69‑1 (MQ=255) gCGGTTGTGCGCCGTCGAAACGACCAAACTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGc < 1:3397466/69‑1 (MQ=255) gCGGTTGTGCGCCGTCGAAACGACCAAACTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGc < 1:344602/69‑1 (MQ=255) gCGGTTGTGCGCCGTCGAAACGACCAAACTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGc < 1:2154487/69‑1 (MQ=255) gCGGTTGTGCGCCGTCGAAACGACCAAACTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGc < 1:2088529/69‑1 (MQ=255) gCGGTTGTGCGCCGTCGAAACGACCAAACTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGc < 1:2612399/69‑1 (MQ=255) gCGGTTGTGCGCCGTCGAAACGACCAAACTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGc < 1:705601/69‑1 (MQ=255) gCGGTTGTGCGCCGTCGAAACGACCAAACTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGc < 1:789157/69‑1 (MQ=255) gCGGTTGTGCGCCGTCGAAACGACCAAACTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGc < 1:160381/69‑1 (MQ=255) gCGGTTGTGCGCCGTCGAAACGACCAAACTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGc < 1:1543700/69‑1 (MQ=255) gCGGTTGTGCGCCGTCGAAACGACCAAACTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGc < 1:1535369/69‑1 (MQ=255) gCGGTTGTGCGCCGTCGAAACGACCAAACTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGc < 1:845436/69‑1 (MQ=255) gCGGTTGTGCGCCGTCGAAACGACCAAACTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGc < 1:144704/69‑1 (MQ=255) gCGGCTCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGc > 1:1844988/1‑69 (MQ=255) gCGGCTCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGc > 1:1874625/1‑69 (MQ=255) gCGGCTCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGc > 1:492798/1‑69 (MQ=255) gCGGCTCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGc > 1:2261177/1‑69 (MQ=255) gCGGCTCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGc > 1:1466429/1‑69 (MQ=255) gCGGCTCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGc > 1:861215/1‑69 (MQ=255) gCGGCTCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGc > 1:1294986/1‑69 (MQ=255) gCGGCTCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGc > 1:2402135/1‑69 (MQ=255) gCGGCTCTGCGCAGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGc > 1:689561/1‑69 (MQ=255) gAAACGACCAAACTTGACGCTTCTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGaa > 1:3022885/1‑69 (MQ=255) gAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCt > 1:2456102/1‑49 (MQ=255) gAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGaa > 1:509079/1‑69 (MQ=255) gAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGaa > 1:907879/1‑69 (MQ=255) gAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGaa > 1:1013994/1‑69 (MQ=255) gAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGaa > 1:290462/1‑69 (MQ=255) gAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGaa > 1:2514313/1‑69 (MQ=255) gAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGaa > 1:29260/1‑69 (MQ=255) gAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGaa > 1:134466/1‑69 (MQ=255) gAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGaa > 1:1519100/1‑69 (MQ=255) gAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGaa > 1:198648/1‑69 (MQ=255) gAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGa > 1:1077956/1‑68 (MQ=255) gAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGa > 1:2489715/1‑68 (MQ=255) gAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATCCCGCCTTTGCCGGTCACCGATTTGa > 1:1856517/1‑68 (MQ=255) aaaCTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGc < 1:3247464/44‑1 (MQ=38) aaCTTGACGCTGCTGCCATCAATAATTTTGATGCCGCCTTTGCCGGTCACCGATTTAAAGc > 1:32838/1‑61 (MQ=255) aaCTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGCCGGTCACCGATTTAAAg > 1:2285500/1‑60 (MQ=255) aaCTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGCCGGTCACCGATTTAAAGc > 1:2369835/1‑61 (MQ=255) aaCTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGCCGGTCACCGATTTAAAGc > 1:2281609/1‑61 (MQ=255) aaCTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGCCGGTCACCGATTTAAAGc > 1:2103939/1‑61 (MQ=255) aaCTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGCCGGTCACCGATTTAAAGc > 1:2028218/1‑61 (MQ=255) aaCTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGCCGGTCACCGATTTAAAGc > 1:3215100/1‑61 (MQ=255) aaCTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGCCGGTCACCGATTTAAAGc > 1:839475/1‑61 (MQ=255) aaCTTGACGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGCCGGTCACCGATTTAAAGc > 1:3193420/1‑61 (MQ=255) aaCTTGACGCTGATGCCATCAATAACTTTGATGCCGCCTTTGCCGGTCACCGATTTAAAGc > 1:2288352/1‑61 (MQ=255) ttGACGCTGCTGCCATCAATAACTTTGTTGCCGCCTTTGc < 1:3029402/40‑1 (MQ=37) aCGCTGCTGCCATCAATAACTTTGATGCCGCCTTTGc < 1:183039/37‑1 (MQ=38) | CAGTCAAGCCCACGCAATGCGGCTCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGC > minE/1620833‑1620937 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |