| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,621,531 | T→C | 60.9% | V65V (GTA→GTG) | eutP ← | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,621,531 | 0 | T | C | 60.9% | 53.7 / 67.2 | 103 | V65V (GTA→GTG) | eutP | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base T (25/15); new base C (23/39); total (49/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
TCCAGCAACCCGGCAGGCAACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATC > minE/1621466‑1621599 | tCCAGCAACCCGGCAGGCAACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTAc > 1:1843862/1‑68 (MQ=255)tCCAGCAACCCGGCAGGCAACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACa > 1:2013254/1‑69 (MQ=255)tCCAGCAACCCGGCAGGCAACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACa > 1:1648880/1‑69 (MQ=255)tCCAGCAACCCGGCAGGCAACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACa > 1:2613206/1‑69 (MQ=255)tCCAGCAACCCGGCAGGCAACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACa > 1:2017102/1‑69 (MQ=255)tCCAGCAACCCGGCAGGCAACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACa > 1:743738/1‑69 (MQ=255)tCCAGCAACCCGGCAGGCAACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACa > 1:2214968/1‑69 (MQ=255)tCCAGCAACCCGGCAGGCAACCGACCTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGGCTACa < 1:3153433/69‑1 (MQ=255) cAGCAACCCGGCAGGCAACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATc > 1:78791/1‑69 (MQ=255) cAGCAACCCGGCAGGCAACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATc > 1:1950123/1‑69 (MQ=255) aaCCCGGCAGGCAACCGACTTTCCGGGTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGt < 1:2191481/69‑1 (MQ=255) aaCCCGGCAGGCAACCGACTTTCCGGGTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGt < 1:2804032/69‑1 (MQ=255) aaCCCGGCAGGCAACCGACTTTCCGGGTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGt < 1:2950222/69‑1 (MQ=255) aaCCCGGCAGGCAACCGACTTTCCGGGTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGt < 1:2953380/69‑1 (MQ=255) aaCCCGGCAGGCAACCGACTTTCCGGGTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGt < 1:2312397/69‑1 (MQ=255) aaCCCGGCAGGCAACCGACTTTCCGGGTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGt < 1:1038250/69‑1 (MQ=255) aggcaACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGt < 1:3201534/69‑1 (MQ=255) aggcaACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGt < 1:1949214/69‑1 (MQ=255) aggcaACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGt < 1:2131052/69‑1 (MQ=255) aggcaACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGt < 1:2885341/69‑1 (MQ=255) caACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGc > 1:788297/1‑61 (MQ=255) caACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTaa > 1:1261011/1‑68 (MQ=255) caACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTaa > 1:2143365/1‑68 (MQ=255) caACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTaa > 1:1578273/1‑68 (MQ=255) caACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTaa > 1:809540/1‑68 (MQ=255) caACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTaa > 1:311719/1‑68 (MQ=255) caACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTaa > 1:1362057/1‑68 (MQ=255) caACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTa > 1:1787695/1‑67 (MQ=255) caACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTa > 1:679419/1‑67 (MQ=255) aCCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTa < 1:2175282/69‑1 (MQ=255) aCCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTa < 1:2079677/69‑1 (MQ=255) aCCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTa < 1:1599147/69‑1 (MQ=255) aCCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTa < 1:438972/69‑1 (MQ=255) aCCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTa < 1:1331760/69‑1 (MQ=255) aCCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTa < 1:799202/69‑1 (MQ=255) tgccGGGTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGt < 1:3378674/66‑1 (MQ=255) tgccGGGTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGt < 1:1985386/66‑1 (MQ=255) tgccGGGTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGt < 1:383097/66‑1 (MQ=255) tgccGGGTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGt < 1:392808/66‑1 (MQ=255) tgccGGGTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGt < 1:646249/66‑1 (MQ=255) gccGGGTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGt < 1:2965682/66‑1 (MQ=255) ggTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATAcc < 1:2811337/69‑1 (MQ=255) ggTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATAcc < 1:361945/69‑1 (MQ=255) ggTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATAcc < 1:427400/69‑1 (MQ=255) ggTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATAcc < 1:922272/69‑1 (MQ=255) ggTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATAcc < 1:2272000/69‑1 (MQ=255) ggTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATAcc < 1:218866/69‑1 (MQ=255) ggTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATAcc < 1:1492837/69‑1 (MQ=255) ggTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATAcc < 1:1986/69‑1 (MQ=255) ggTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATAcc < 1:1344336/69‑1 (MQ=255) ggTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATAcc < 1:1787442/69‑1 (MQ=255) ggTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATAcc < 1:1351422/69‑1 (MQ=255) ggTCATTCGCGCCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATAcc < 1:1936064/69‑1 (MQ=255) gcgcCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTa < 1:462121/49‑1 (MQ=255) gcCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAAtt < 1:1539452/46‑1 (MQ=255) gcCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAAtt < 1:1549990/46‑1 (MQ=255) gcCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAAtt < 1:1534598/46‑1 (MQ=255) gcCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAAtt < 1:491088/46‑1 (MQ=255) gcCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAAtt < 1:2985101/46‑1 (MQ=255) gcCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAAtt < 1:527687/46‑1 (MQ=255) gcCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAAtt < 1:562852/46‑1 (MQ=255) gcCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAAtt < 1:2720596/46‑1 (MQ=255) gcCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAAtt < 1:1329663/46‑1 (MQ=255) gcCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAAtt < 1:252682/46‑1 (MQ=255) gcCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAAtt < 1:2505341/46‑1 (MQ=255) gcCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAAtt < 1:2132364/46‑1 (MQ=255) gcCGTGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAAtt < 1:838163/46‑1 (MQ=255) gcCATGGACATAGATCAGCATGTCCACATCCTGTAGCGTGGTAAtt < 1:1535743/46‑1 (MQ=255) gACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTaa > 1:1985159/1‑68 (MQ=255) aTAGATCAGCATGTCCACATCCTGTAGCGTGGTAAtt < 1:1347077/37‑1 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:703338/1‑54 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:671696/1‑54 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:531517/1‑54 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:1161577/1‑54 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:1088177/1‑54 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:1078472/1‑54 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:1645772/1‑54 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:3396471/1‑54 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:2200007/1‑54 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:2056273/1‑54 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:253071/1‑54 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:2630872/1‑54 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:2645988/1‑54 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:2696179/1‑54 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:2744468/1‑54 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:1767775/1‑54 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:3018625/1‑54 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:3057597/1‑54 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:3101487/1‑54 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:1679623/1‑54 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:1612288/1‑54 (MQ=255) aGATCAGCATGTCCACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:1544301/1‑54 (MQ=255) aGATCAGCATGTCAACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCggg > 1:1377045/1‑54 (MQ=255) gATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTc > 1:1687913/1‑69 (MQ=255) gATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTc > 1:2875835/1‑69 (MQ=255) gATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTc > 1:1826064/1‑69 (MQ=255) gATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTc > 1:502524/1‑69 (MQ=255) cAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGAt < 1:562835/53‑1 (MQ=255) cAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGAt < 1:2170178/53‑1 (MQ=255) tACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATc < 1:1739630/69‑1 (MQ=255) | TCCAGCAACCCGGCAGGCAACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATC > minE/1621466‑1621599 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |