| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,622,426 | C→T | 24.2% | V740V (GTG→GTA) | maeB ← | fused malic enzyme predicted oxidoreductase and predicted phosphotransacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,622,426 | 0 | C | T | 24.2% | 99.9 / 24.6 | 78 | V740V (GTG→GTA) | maeB | fused malic enzyme predicted oxidoreductase and predicted phosphotransacetylase |
| Reads supporting (aligned to +/- strand): ref base C (35/24); new base T (6/13); total (41/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.28e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
TACAGCGGTTGGGTTTGCGCTTCTACCACGGCCAGCGCCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACAGTCAC > minE/1622367‑1622494 | tACAGCGGTTGGGTTTGCGCTTCTACCACGGCCAGCGCCACCATGTTGACGATACGACGCACCGATGCg < 1:2238232/69‑1 (MQ=255)tACAGCGGTTGGGTTTGCGCTTCTACCACGGCCAGCGCCACCATGTTGACGATACGACGCACCGATGCg < 1:634972/69‑1 (MQ=255) aCAGCGGTTGGGTTTGCGCTTCTACCACGGCCAGCGCCACCATGTTGACGATACGACGCACCGATGCGa > 1:1777530/1‑69 (MQ=255) aCAGCGGTTGGGTTTGCGCTTCTACCACGGCCAGCGCCACCATGTTGACGATACGACGCACCGATGCGa > 1:2373330/1‑69 (MQ=255) aCAGCGGTTGGGTTTGCGCTTCTACCACGGCCAGCGCCACCATGTTGACGATACGACGCACCGATGCGa > 1:34851/1‑69 (MQ=255) aCAGCGGTTGGGTTTGCGCTTCTACCACGGCCAGCGCCACCATGTTGACGATACGACGCACCGATGCGa > 1:135820/1‑69 (MQ=255) aCAGCGGTTGGGTTTGCGCTTCTACCACGGCCAGCGCCACCATGTTGACGATACGACGCACCGATGCGa > 1:1529833/1‑69 (MQ=255) aCAGCGGTTGGGTTTGCGCTTCTACCACGGCCAGCGCCACCATGTTGACGATACGACGCACCGATGCGa > 1:1450823/1‑69 (MQ=255) gCGGTTGGGTTTGCGCTTCTACCACGGCCAGCGCCACCATGTTGACGATACGACGCACCGATGCGATCg < 1:2953735/69‑1 (MQ=255) ttGCGCTTCTACCACGGCCAGCGCCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAacac < 1:1107391/69‑1 (MQ=255) gcgcTTCAACCACGGCCAGCGCCACCATGTTGACGATACGACGTAc > 1:296159/1‑46 (MQ=39) gcgcTTCAACCACGGCCAGCGCCACCATGTTGACGATACGACGTAc > 1:1748883/1‑46 (MQ=39) gcgcTTCAACCACGGCCAGCGCCACCATGTTGACGATACGACGTAc > 1:3292668/1‑46 (MQ=39) gcgcTTCAACCACGGCCAGCGCCACCATGTTGACGATACGACGTAc > 1:3417998/1‑46 (MQ=39) gcgcTTCAACCACGGCCAGCGCCACCATGTTGACGATACGACGTAc > 1:2448068/1‑46 (MQ=39) gcgcTTCAACCACGGCCAGCGCCACCATGTTGACGATACGACGTAc > 1:892585/1‑46 (MQ=39) tCTACCACGGCCAGCGCCACCATGTTGACGATACGACGCACCGATGCGATCg < 1:2516679/52‑1 (MQ=255) cAGCGCCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCcac < 1:2742897/69‑1 (MQ=255) gcgcCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCacac < 1:297970/69‑1 (MQ=255) gcgcCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCacac < 1:3286472/69‑1 (MQ=255) gcgcCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCacac < 1:3227803/69‑1 (MQ=255) ccaTGTTGACGATACGACGTACCTATGCGATCGGCGTTAACACGTGAACCGGt < 1:3392074/53‑1 (MQ=39) ccaTGTTGACGATACGACGTACCGATGCGATCGGCGTTAACAGGTGAACCGGt < 1:1054396/53‑1 (MQ=255) ccaTGTTGACGATACGACGTACCGATGCGATCGGCGTTAACACGTGAACCGGt < 1:1676554/53‑1 (MQ=255) ccaTGTTGACGATACGACGTACCGATGCGATCGGCGTTAACACGTGAACCGGt < 1:249947/53‑1 (MQ=255) ccaTGTTGACGATACGACGTACCGATGCGATCGGCGTTAACACGTGAACCGGt < 1:2837491/53‑1 (MQ=255) ccaTGTTGACGATACGACGTACCGATGCGATCGGCGTTAACACGTGAACCGGt < 1:2918916/53‑1 (MQ=255) ccaTGTTGACGATACGACGTACCGATGCGATCGGCGTTAACACGTGAACCGGt < 1:382341/53‑1 (MQ=255) ccaTGTTGACGATACGACGTACCGATGCGATCGGCGTTAACACGTGAACCGGt < 1:379491/53‑1 (MQ=255) ccaTGTTGACGATACGACGTACCGATGCGATCGGCGTTAACACGTGAACCGGt < 1:1379258/53‑1 (MQ=255) ccaTGTTGACGATACGACGTACCGATGCGATCGGCGTTAACACGTGAACCGGt < 1:2031975/53‑1 (MQ=255) ccaTGTTGACGATACGACGTACCGATGCGATCGGCGTTAACACGTGAACCGGt < 1:3189831/53‑1 (MQ=255) ccaTGTTGACGATACGACGTACCGATGCGATCGGCGTTAACACGTGAACCGGt < 1:2201188/53‑1 (MQ=255) ccaTGTTGACGATACGACGTACCGATGCGATCGGCGTTAACACGTGAACCGGt < 1:2144389/53‑1 (MQ=255) ttGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTc > 1:2057828/1‑51 (MQ=255) ttGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACAcc > 1:3026636/1‑59 (MQ=255) ttGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACGCATCAGCAcc > 1:2267017/1‑69 (MQ=255) ttGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCAcc > 1:310570/1‑69 (MQ=255) ttGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCAcc > 1:559221/1‑69 (MQ=255) ttGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCAcc > 1:3123638/1‑69 (MQ=255) ttGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCAcc > 1:796289/1‑69 (MQ=255) ttGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCAcc > 1:2474289/1‑69 (MQ=255) ttGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCAcc > 1:10278/1‑69 (MQ=255) ttGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCAcc > 1:1331279/1‑69 (MQ=255) ttGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCAcc > 1:1309065/1‑69 (MQ=255) ttGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCAcc > 1:1136877/1‑69 (MQ=255) ttGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCAcc > 1:2114805/1‑69 (MQ=255) ttGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCAcc > 1:2234089/1‑69 (MQ=255) tACGACGCACCGATGCGATCGGCGTTAACACGTGATCCGGTTTCGCCACACCCATCAGCACCGGGcc > 1:2106054/1‑67 (MQ=255) tACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGcc > 1:1559076/1‑67 (MQ=255) tACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGcc > 1:3307053/1‑67 (MQ=255) tACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGcc > 1:397670/1‑67 (MQ=255) tACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGcc > 1:1280040/1‑67 (MQ=255) tACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGcc > 1:202385/1‑67 (MQ=255) tACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGcc > 1:2328693/1‑67 (MQ=255) tACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGcc > 1:734902/1‑67 (MQ=255) tACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGcc > 1:2385991/1‑67 (MQ=255) tACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGcc > 1:854538/1‑67 (MQ=255) tACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGcc > 1:2825470/1‑67 (MQ=255) tACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGcc > 1:2464116/1‑67 (MQ=255) tACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGcc > 1:1111328/1‑67 (MQ=255) tACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGcc > 1:2541673/1‑67 (MQ=255) acgacgCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCGGAc > 1:721709/1‑69 (MQ=255) cgacgCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACa < 1:131038/69‑1 (MQ=255) cgacgCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACa < 1:997050/69‑1 (MQ=255) cgacgCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACa < 1:1811306/69‑1 (MQ=255) cgacgCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACa < 1:2958535/69‑1 (MQ=255) cgacgCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACa < 1:2389116/69‑1 (MQ=255) acgCACCTATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACAGt < 1:2624692/69‑1 (MQ=255) acgCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACAGt < 1:1384657/69‑1 (MQ=255) acgCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACAGt < 1:1700521/69‑1 (MQ=255) acgCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACAGt < 1:3278127/69‑1 (MQ=255) acgCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACAGt < 1:199850/69‑1 (MQ=255) acgCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACAGt < 1:121577/69‑1 (MQ=255) acgCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACAGt < 1:660323/69‑1 (MQ=255) acgCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACAGt < 1:2275939/69‑1 (MQ=255) acgCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACAGt < 1:2451/69‑1 (MQ=255) acgCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACAGt < 1:2486792/69‑1 (MQ=255) cACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACAGTcac < 1:3319379/69‑1 (MQ=255) cACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACAGTcac < 1:556850/69‑1 (MQ=255) cACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACAGTcac < 1:938154/69‑1 (MQ=255) | TACAGCGGTTGGGTTTGCGCTTCTACCACGGCCAGCGCCACCATGTTGACGATACGACGCACCGATGCGATCGGCGTTAACACGTGAACCGGTTTCGCCACACCCATCAGCACCGGGCCGACAGTCAC > minE/1622367‑1622494 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |